Attempts at the development of a recombinant African swine fever virus strain with abrogated EP402R, 9GL , and A238L gene structure using the CRISPR/Cas9 system
Published Online: Jun 03, 2020
Page range: 197 - 205
Received: Oct 04, 2019
Accepted: May 25, 2020
DOI: https://doi.org/10.2478/jvetres-2020-0039
Keywords
© 2020 G. Woźniakowski et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Introduction
African swine fever (ASF) is a pressing economic problem in a number of Eastern European countries. It has also depleted the Chinese sow population by 50%. Managing the disease relies on culling infected pigs or hunting wild boars as sanitary zone creation. The constraints on the development of an efficient vaccine are mainly the virus’ mechanisms of host immune response evasion. The study aimed to adapt a field ASFV strain to established cell lines and to construct recombinant African swine fever virus (ASFV) strain.
Material and Methods
The host immune response modulation genes
Results
The reconstituted virus showed similar kinetics of replication in comparison to the parent virus isolate.
Conclusion
Taking into account the usefulness of the developed CRISPR/Cas9 system it has been shown that modification of the