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Changes in the expression of TLR2 during the intestinal phase of trichinellosis

Agnieszka Wojtkowiak-Giera

Agnieszka Wojtkowiak-Giera

Department of Biology and Medical Parasitology, Poznan University of Medical SciencesPoznań, Poland
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Wojtkowiak-Giera, Agnieszka
, 
Monika Derda

Monika Derda

Department of Biology and Medical Parasitology, Poznan University of Medical SciencesPoznań, Poland
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Derda, Monika
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Elżbieta Wandurska-Nowak

Elżbieta Wandurska-Nowak

Department of Biology and Medical Parasitology, Poznan University of Medical SciencesPoznań, Poland
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Wandurska-Nowak, Elżbieta
, 
Paweł P. Jagodziński

Paweł P. Jagodziński

Department of Biochemistry and Molecular Biology, Poznan University of Medical SciencesPoznań, Poland
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Jagodziński, Paweł P.
, 
Agnieszka Kolasa-Wołosiuk

Agnieszka Kolasa-Wołosiuk

Department of Histology and Embryology, Pomeranian Medical UniversitySzczecin, Poland
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Kolasa-Wołosiuk, Agnieszka
, 
Danuta Kosik-Bogacka

Danuta Kosik-Bogacka

Independent of Pharmaceutical Botany, Department of Biology and Medical Parasitology, Pomeranian Medical UniversitySzczecin, Poland
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Kosik-Bogacka, Danuta
 and 
Edward Hadaś

Edward Hadaś

Department of Biology and Medical Parasitology, Poznan University of Medical SciencesPoznań, Poland
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Hadaś, Edward
  
Apr 21, 2020
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Published Online: Apr 21, 2020
Page range: 269 - 274
Received: May 22, 2019
Accepted: Apr 03, 2020
DOI: https://doi.org/10.2478/jvetres-2020-0027
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© 2020 A. Wojtkowiak-Giera et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Representative microphotography showing expression of TLR2 at the protein level in intestines isolated from control (uninfected) and T. spiralis-infected miceA – jejunum of T. spiralis-infected mouse at 4 dpi with many brown-stained, TLR2-positive epithelial cells (20×);B – jejunum of T. spiralis-infected mouse at 8 dpi with similar, strong immunohistochemical positive reaction indicating presence of TLR2 protein in epithelial cells (40×);C – jejunum from control (uninfected) mouse with almost no positive reaction for TLR2 protein (10×);D – colon from uninfected mouse also with almost no positive reaction for TLR2 (20x)
Representative microphotography showing expression of TLR2 at the protein level in intestines isolated from control (uninfected) and T. spiralis-infected miceA – jejunum of T. spiralis-infected mouse at 4 dpi with many brown-stained, TLR2-positive epithelial cells (20×);B – jejunum of T. spiralis-infected mouse at 8 dpi with similar, strong immunohistochemical positive reaction indicating presence of TLR2 protein in epithelial cells (40×);C – jejunum from control (uninfected) mouse with almost no positive reaction for TLR2 protein (10×);D – colon from uninfected mouse also with almost no positive reaction for TLR2 (20x)

Fig 2

Trends of changes in mRNA expression of the tlr2 gene in the intestinal phase of T. spiralis infection
Trends of changes in mRNA expression of the tlr2 gene in the intestinal phase of T. spiralis infection

Expression of tlr 2 at the mRNA level in the intestines of uninfected and T_ spiralis-infected mice determined using qPCR

Days post infection (dpi)Expression of mRNA tlr2 in the jejunumExpression of mRNA tlr2 in the colon
0 (uninfected)0.7 ± 0.190.6 ± 0.16
41.5 ± 0.24*0.7 ± 0.04
81.4 ± 0.26*0.6 ± 0.07
161.4 ± 0.23*0.6 ± 0.10
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English
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Journal Subjects:
Life Sciences, Molecular Biology, Microbiology and Virology, Life Sciences, other, Medicine, Veterinary Medicine
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