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Molecular study of Cysticercus tenuicollis from slaughtered sheep in Sulaymaniyah province, Iraq

Aram Ahmad Mohammed

Aram Ahmad Mohammed

Department of Microbiology, College of Veterinary Medicine, Sulaimani UniversitySulaymaniyah City, Iraq
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Mohammed, Aram Ahmad
  
May 12, 2020
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Published Online: May 12, 2020
Page range: 275 - 280
Received: Sep 07, 2019
Accepted: May 04, 2020
DOI: https://doi.org/10.2478/jvetres-2020-0034
Keywords
© 2020 Aram Ahmad Mohammed. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

Cysticercosis caused by the larval stage of Taenia hydatigena is economically the most important endemic parasitic disease in Iraq. Few data are available relating to the genetic divergence of this helminth. This study aimed to molecularly characterise Cysticercus tenuicollis isolates from sheep in Sulaymaniyah province, Iraq.

Material and Methods

DNA extraction and amplification of specimens of C. tenuicollis from 46 sheep were conducted by PCR for the mitochondrial 12S rRNA gene. The 19 amplicons were subjected to purification and partial sequencing.

Results

Five 12S rRNA nucleotide sequence haplotypes were found. The pairwise nucleotide difference between haplotypes of 12S rRNA gene ranged from 0.2% to 0.7%. Four out of the five haplotypes of C. tenuicollis contained one to two base mutations and were discovered in Iraq for the first time, and this may be a unique mutation globally which has not been recorded previously. Three newly recorded haplotypes contained only one single mutation, and the other one contained two mutations. Phylogenetic analysis showed that all isolated strains were closely related to Iranian sheep isolates.

Conclusions

Four new strains of T. hydatigena were discovered for the first time in the study area.

Language:
English
Publication timeframe:
4 times per year
Journal Subjects:
Life Sciences, Molecular Biology, Microbiology and Virology, Life Sciences, other, Medicine, Veterinary Medicine
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