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Elaboration of triplex PCR for detection of selected viral infections in waterfowl

Wojciech Kozdruń

Wojciech Kozdruń

Department of Poultry Diseases, National Veterinary Research InstitutePuławy, Poland
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Kozdruń, Wojciech
, 
Hanna Czekaj

Hanna Czekaj

Department of Poultry Diseases, National Veterinary Research InstitutePuławy, Poland
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Czekaj, Hanna
, 
Natalia Styś-Fijoł

Natalia Styś-Fijoł

Department of Poultry Diseases, National Veterinary Research InstitutePuławy, Poland
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Styś-Fijoł, Natalia
, 
Karolina Piekarska

Karolina Piekarska

Department of Poultry Diseases, National Veterinary Research InstitutePuławy, Poland
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Piekarska, Karolina
 and 
Jowita Samanta Niczyporuk

Jowita Samanta Niczyporuk

Department of Poultry Diseases, National Veterinary Research InstitutePuławy, Poland
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Samanta Niczyporuk, Jowita
  
Dec 19, 2019
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Article Category: Short communication
Published Online: Dec 19, 2019
Page range: 503 - 505
Received: Jul 17, 2019
Accepted: Nov 22, 2019
DOI: https://doi.org/10.2478/jvetres-2019-0074
Keywords
© 2019 W. Kozdruń et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Results of electrophoresis of optimal triplex PCR for detection of DNA of GPV, GHPV, and GoCV Lanes: 1 – DNA marker; 2 – GPV; 3 – GoCV; 4 – GHPV; 5 – GPV, GoCV, GHPV; 6 – GPV, GoCV; 7 – GPV, GHPV; 8 – GoCV, GHPV; 9 – GPV, GoCV, GHPV; 10 – uninfected GEF (negative control)
Results of electrophoresis of optimal triplex PCR for detection of DNA of GPV, GHPV, and GoCV Lanes: 1 – DNA marker; 2 – GPV; 3 – GoCV; 4 – GHPV; 5 – GPV, GoCV, GHPV; 6 – GPV, GoCV; 7 – GPV, GHPV; 8 – GoCV, GHPV; 9 – GPV, GoCV, GHPV; 10 – uninfected GEF (negative control)

Optimal parameters of triplex PCR

Parameter Volume of parameter
Concentration of Mg2+ 3 mM
Temperature of primers 60°C
Number of cycles 37
Concentration of Taq polymerase DNA 1 U
Language:
English
Publication timeframe:
4 times per year
Journal Subjects:
Life Sciences, Molecular Biology, Microbiology and Virology, Life Sciences, other, Medicine, Veterinary Medicine
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