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Elaboration of triplex PCR for detection of selected viral infections in waterfowl

Wojciech Kozdruń

Wojciech Kozdruń

Department of Poultry Diseases, National Veterinary Research InstitutePuławy, Poland
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Kozdruń, Wojciech
, 
Hanna Czekaj

Hanna Czekaj

Department of Poultry Diseases, National Veterinary Research InstitutePuławy, Poland
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Czekaj, Hanna
, 
Natalia Styś-Fijoł

Natalia Styś-Fijoł

Department of Poultry Diseases, National Veterinary Research InstitutePuławy, Poland
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Styś-Fijoł, Natalia
, 
Karolina Piekarska

Karolina Piekarska

Department of Poultry Diseases, National Veterinary Research InstitutePuławy, Poland
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Piekarska, Karolina
 e 
Jowita Samanta Niczyporuk

Jowita Samanta Niczyporuk

Department of Poultry Diseases, National Veterinary Research InstitutePuławy, Poland
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Samanta Niczyporuk, Jowita
  
19 dic 2019
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Categoria dell'articolo: Short communication
Pubblicato online: 19 dic 2019
Pagine: 503 - 505
Ricevuto: 17 lug 2019
Accettato: 22 nov 2019
DOI: https://doi.org/10.2478/jvetres-2019-0074
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© 2019 W. Kozdruń et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Results of electrophoresis of optimal triplex PCR for detection of DNA of GPV, GHPV, and GoCV Lanes: 1 – DNA marker; 2 – GPV; 3 – GoCV; 4 – GHPV; 5 – GPV, GoCV, GHPV; 6 – GPV, GoCV; 7 – GPV, GHPV; 8 – GoCV, GHPV; 9 – GPV, GoCV, GHPV; 10 – uninfected GEF (negative control)
Results of electrophoresis of optimal triplex PCR for detection of DNA of GPV, GHPV, and GoCV Lanes: 1 – DNA marker; 2 – GPV; 3 – GoCV; 4 – GHPV; 5 – GPV, GoCV, GHPV; 6 – GPV, GoCV; 7 – GPV, GHPV; 8 – GoCV, GHPV; 9 – GPV, GoCV, GHPV; 10 – uninfected GEF (negative control)

Optimal parameters of triplex PCR

Parameter Volume of parameter
Concentration of Mg2+ 3 mM
Temperature of primers 60°C
Number of cycles 37
Concentration of Taq polymerase DNA 1 U
Lingua:
Inglese
Frequenza di pubblicazione:
4 volte all'anno
Argomenti della rivista:
Scienze biologiche, Biologia molecolare, Microbiologia e virologia, Scienze della vita, altro, Medicina, Medicina veterinaria
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