<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_jvetres-2018-0041_s_005_w2aab3b7b8b1b6b1aab1c16b1Aa"><h3>Introduction</h3><p>Genes related to iron metabolism play an important role in inflammatory response. The objective of this study was to investigate the role of ferritin, transferrin receptors 1a and 1b, and transferrin genes in the response to blood parasite infection in common carp (<italic>Cyprinuscarpio</italic> L.).</p></sec><sec id="j_jvetres-2018-0041_s_006_w2aab3b7b8b1b6b1aab1c16b2Aa"><h3>Material and Methods</h3><p>Two genetically distinct carp groups were used: R3 carp, which are established as being sensitive to parasitic infection, and SA carp (<italic>Cyprinus carpio haematopterus</italic>) of wild origin. An established challenge model with <italic>Trypanoplasma borreli</italic> was applied. Challenged carp were sampled to determine their expression levels of transferrin receptors 1a and 1b, ferritin, and transferrin mRNA. Mortality and serum iron concentration were also measured.</p></sec><sec id="j_jvetres-2018-0041_s_007_w2aab3b7b8b1b6b1aab1c16b3Aa"><h3>Results</h3><p>The study revealed contrasting differences in the expression profiles of all key iron regulatory genes except the transferrin gene. In the case of other parameters, significant differences were also observed.</p></sec><sec id="j_jvetres-2018-0041_s_008_w2aab3b7b8b1b6b1aab1c16b4Aa"><h3>Conclusion</h3><p>Our results demonstrate that the level of parasitic infection depends on the blood iron status. This parameter was related to the origin of the fish.</p></sec></abstract>

IntroductionGenes related to iron metabolism play an important role in inflammatory response. The objective of this study was to investigate the role of ferritin, transferrin receptors 1a and 1b, and transferrin genes in the response to blood parasite infection in common carp (Cyprinuscarpio L.).Material and MethodsTwo genetically distinct carp groups were used: R3 carp, which are established as being sensitive to parasitic infection, and SA carp (Cyprinus carpio haematopterus) of wild origin. An established challenge model with Trypanoplasma borreli was applied. Challenged carp were sampled to determine their expression levels of transferrin receptors 1a and 1b, ferritin, and transferrin mRNA. Mortality and serum iron concentration were also measured.ResultsThe study revealed contrasting differences in the expression profiles of all key iron regulatory genes except the transferrin gene. In the case of other parameters, significant differences were also observed.ConclusionOur results demonstrate that the level of parasitic infection depends on the blood iron status. This parameter was related to the origin of the fish.

Influence of the genetic makeup of common carp on the expression of iron-related genes during <italic>Trypanoplasma borreli</italic> infection

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Influence of the genetic makeup of common carp on the expression of iron-related genes during  infection

IntroductionGenes related to iron metabolism play an important role in inflammatory response. The objective of this study was to investigate the role of...

Influence of the genetic makeup of common carp on the expression of iron-related genes during Trypanoplasma borreli infection

Published Online: Oct 23, 2018

Page range: 285 - 290

Received: May 16, 2018

Accepted: Sep 18, 2018

DOI: https://doi.org/10.2478/jvetres-2018-0041

Keywords
carp, gene expression, transferrin,

© 2018 T. Kamińska-Gibas et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Influence of the genetic makeup of common carp on the expression of iron-related genes during Trypanoplasma borreli infection

Published Online: Oct 23, 2018

Page range: 285 - 290

Received: May 16, 2018

Accepted: Sep 18, 2018

DOI: https://doi.org/10.2478/jvetres-2018-0041

Keywordscarp, gene expression, transferrin,

© 2018 T. Kamińska-Gibas et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
carp, gene expression, transferrin,