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Morphological and Molecular Characterization of Punctodera stonei Brzeski, 1998 (Nematoda: Heteroderidae) from Virginia, USA

Mihail R. Kantor

Mihail R. Kantor

Mycology and Nematology Genetic Diversity and Biology Laboratory, USDA, ARS, Northeast AreaBeltsville, USA
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Kantor, Mihail R.
, 
Sergei A. Subbotin

Sergei A. Subbotin

  • California Department of Food and Agriculture, Plant Pest Diagnostic CenterSacramento, USA
  • Center of Parasitology of A.N. Severtsov Institute of Ecology and Evolution of the Russian Academy of SciencesMoscow, Russia
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Subbotin, Sergei A.
, 
Gregory Huse

Gregory Huse

Arlington National CemeteryArlington, USA
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Huse, Gregory
 and 
Zafar A. Handoo

Zafar A. Handoo

Mycology and Nematology Genetic Diversity and Biology Laboratory, USDA, ARS, Northeast AreaBeltsville, USA
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Handoo, Zafar A.
  
Apr 16, 2022
Morphological and Molecular Characterization of Punctodera stonei Brzeski, 1998 (Nematoda: Heteroderidae) from Virginia, USA's Cover Image
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Article Category: Research Paper
Published Online: Apr 16, 2022
Received: Dec 20, 2021
DOI: https://doi.org/10.2478/jofnem-2022-0008
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© 2022 Mihail R. Kantor et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1

Photomicrographs of cysts, vulval cones, and J2 of P. stonei from Virginia. A, B: Anterior ends of J2s; C: Excretory pore and hemizonid with arrow pointing toward hemizonid; D, E: Entire cysts with D showing both fenestra in the middle; F: Cyst posterior part showing vulval and anal fenestrae; G: Lateral field with four incisures for J2; H, I: Tails of J2s with arrow pointing toward the anal area in I.J2, second-stage juveniles.
Photomicrographs of cysts, vulval cones, and J2 of P. stonei from Virginia. A, B: Anterior ends of J2s; C: Excretory pore and hemizonid with arrow pointing toward hemizonid; D, E: Entire cysts with D showing both fenestra in the middle; F: Cyst posterior part showing vulval and anal fenestrae; G: Lateral field with four incisures for J2; H, I: Tails of J2s with arrow pointing toward the anal area in I.J2, second-stage juveniles.

Figure 2

Phylogenetic relationships within the genus Punctodera: Bayesian 50% majority rule consensus tree from two runs, as inferred from analysis of the D2–D3 of 28S rRNA gene sequence alignment under the GTR + I + G model. Posterior probabilities and bootstrap values ≥70% are given for appropriate clades. New sequences are indicated by bold font.
Phylogenetic relationships within the genus Punctodera: Bayesian 50% majority rule consensus tree from two runs, as inferred from analysis of the D2–D3 of 28S rRNA gene sequence alignment under the GTR + I + G model. Posterior probabilities and bootstrap values ≥70% are given for appropriate clades. New sequences are indicated by bold font.

Figure 3

Phylogenetic relationships within the genus Punctodera: Bayesian 50% majority rule consensus tree from two runs, as inferred from analysis of the ITS rRNA gene sequence alignment under the GTR + I + G model. Posterior probabilities and bootstrap values ≥70% are given for appropriate clades. New sequences are indicated by bold font. *Identified as P. punctata in the GenBank and by Sabo et al. (2002).
Phylogenetic relationships within the genus Punctodera: Bayesian 50% majority rule consensus tree from two runs, as inferred from analysis of the ITS rRNA gene sequence alignment under the GTR + I + G model. Posterior probabilities and bootstrap values ≥70% are given for appropriate clades. New sequences are indicated by bold font. *Identified as P. punctata in the GenBank and by Sabo et al. (2002).

Figure 4

Phylogenetic relationships within the genus Punctodera: Bayesian 50% majority rule consensus tree from two runs, as inferred from analysis of the COI gene sequence alignment under the GTR + I + G model. Posterior probabilities and bootstrap values ≥70% are given for appropriate clades. New sequences are indicated by bold font.
Phylogenetic relationships within the genus Punctodera: Bayesian 50% majority rule consensus tree from two runs, as inferred from analysis of the COI gene sequence alignment under the GTR + I + G model. Posterior probabilities and bootstrap values ≥70% are given for appropriate clades. New sequences are indicated by bold font.
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