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Morphological and Molecular Characterization of Punctodera stonei Brzeski, 1998 (Nematoda: Heteroderidae) from Virginia, USA

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Figure 1

Photomicrographs of cysts, vulval cones, and J2 of P. stonei from Virginia. A, B: Anterior ends of J2s; C: Excretory pore and hemizonid with arrow pointing toward hemizonid; D, E: Entire cysts with D showing both fenestra in the middle; F: Cyst posterior part showing vulval and anal fenestrae; G: Lateral field with four incisures for J2; H, I: Tails of J2s with arrow pointing toward the anal area in I.J2, second-stage juveniles.
Photomicrographs of cysts, vulval cones, and J2 of P. stonei from Virginia. A, B: Anterior ends of J2s; C: Excretory pore and hemizonid with arrow pointing toward hemizonid; D, E: Entire cysts with D showing both fenestra in the middle; F: Cyst posterior part showing vulval and anal fenestrae; G: Lateral field with four incisures for J2; H, I: Tails of J2s with arrow pointing toward the anal area in I.J2, second-stage juveniles.

Figure 2

Phylogenetic relationships within the genus Punctodera: Bayesian 50% majority rule consensus tree from two runs, as inferred from analysis of the D2–D3 of 28S rRNA gene sequence alignment under the GTR + I + G model. Posterior probabilities and bootstrap values ≥70% are given for appropriate clades. New sequences are indicated by bold font.
Phylogenetic relationships within the genus Punctodera: Bayesian 50% majority rule consensus tree from two runs, as inferred from analysis of the D2–D3 of 28S rRNA gene sequence alignment under the GTR + I + G model. Posterior probabilities and bootstrap values ≥70% are given for appropriate clades. New sequences are indicated by bold font.

Figure 3

Phylogenetic relationships within the genus Punctodera: Bayesian 50% majority rule consensus tree from two runs, as inferred from analysis of the ITS rRNA gene sequence alignment under the GTR + I + G model. Posterior probabilities and bootstrap values ≥70% are given for appropriate clades. New sequences are indicated by bold font. *Identified as P. punctata in the GenBank and by Sabo et al. (2002).
Phylogenetic relationships within the genus Punctodera: Bayesian 50% majority rule consensus tree from two runs, as inferred from analysis of the ITS rRNA gene sequence alignment under the GTR + I + G model. Posterior probabilities and bootstrap values ≥70% are given for appropriate clades. New sequences are indicated by bold font. *Identified as P. punctata in the GenBank and by Sabo et al. (2002).

Figure 4

Phylogenetic relationships within the genus Punctodera: Bayesian 50% majority rule consensus tree from two runs, as inferred from analysis of the COI gene sequence alignment under the GTR + I + G model. Posterior probabilities and bootstrap values ≥70% are given for appropriate clades. New sequences are indicated by bold font.
Phylogenetic relationships within the genus Punctodera: Bayesian 50% majority rule consensus tree from two runs, as inferred from analysis of the COI gene sequence alignment under the GTR + I + G model. Posterior probabilities and bootstrap values ≥70% are given for appropriate clades. New sequences are indicated by bold font.
eISSN:
2640-396X
Langue:
Anglais
Périodicité:
Volume Open
Sujets de la revue:
Life Sciences, other