Effect of Time Between Carbon Dioxide Treatments on the Onset of Oviposition in Queen Honey Bees

Onset of oviposition by virgin queens depending on storage conditions in time between two CO2 treatments (days after treatments)

Storage conditions*	Number of queens	Min - Max	Mean ± sd	Median
Nuclei	12	6 - 29	12.5 ± 7.34	10 a
Incubator	11	6 - 35	21.7 ± 9.35	17 b
Overall	23	6 - 35	16.9 ± 9.43	14

Onset of oviposition by instrumentally inseminated queens and virgins (days after second CO2 treatment)

Queens	Number of queens	Min - Max	Mean ± sd	Median
Inseminated	95	2 - 35	12.0 ± 8.87	9a
Virgins	100	2 - 41	11.7 ± 8.48	9a
Overall	195	2 - 41	11.9 ± 8.65	9

Onset of oviposition of queens depending on time between first and second CO2 treatment (days after second treatment)

Time between treatments	Number of queens	Min - Max	Mean ± sd	Median
96 hrs	18	3 - 33	10.3 ± 7.84	9a
48 hrs	22	2 - 26	7.6 ± 5.05	7a
24 hrs	29	2 - 34	9.9 ± 7.65	7a
12 hrs	19	2 - 30	10.5 ± 8.60	8a
6 hrs	19	2 - 17	7.8 ± 4.47	7a
3 hrs	22	2 - 41	10.9 ± 9.48	8.5a
10 min*	30	4 - 35	14.2 ± 7.98	11.5b
5–6 sec*	19	3 - 37	15.0 ± 7.87	13b
Control**	17	6 - 41	21.9 ± 10.52	21c
Overall	195	2 - 41	11.9 ± 8.65	9