<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>It is commonly known that two carbon dioxide treatments accelerate the beginning of egg laying in honey bee queens. However, the minimum time between anaesthesias needed for ovary activation has not yet been examined. The aim of the study was to investigate the effect of time between carbon dioxide treatments on the onset of oviposition in instrumentally inseminated and virgin queen honey bees. About half of the queens were anaesthetised before insemination and a second time during insemination, and the rest of the queens were anaesthetised two times without insemination. The time between carbon dioxide treatments was as follows: 96, 48, 24, 12, 6, 3 hrs, 10 min, 5–6 sec. Queens in a control group were anaesthetised only once. The mean number of days between the second treatment and the onset of oviposition in particular groups and in the control group was 10.3, 7.6, 9.9, 10.5, 7.8, 10.9, 14.2, 15 and 21.9, respectively, and the median was 9, 7, 7, 8, 7, 8.5, 11.5, 13 and 21 days, respectively. Queens in groups 5–6 sec and 10 min began oviposition significantly earlier than those in control group, but significantly later than in groups from 3 to 96 hrs. Instrumentally inseminated queens started to lay eggs at the same time (mean 12 and median 9 days after second treatment) as queens treated with carbon dioxide without insemination (mean 11.7 and median 9 days).</p>
</abstract>

It is commonly known that two carbon dioxide treatments accelerate the beginning of egg laying in honey bee queens. However, the minimum time between anaesthesias needed for ovary activation has not yet been examined. The aim of the study was to investigate the effect of time between carbon dioxide treatments on the onset of oviposition in instrumentally inseminated and virgin queen honey bees. About half of the queens were anaesthetised before insemination and a second time during insemination, and the rest of the queens were anaesthetised two times without insemination. The time between carbon dioxide treatments was as follows: 96, 48, 24, 12, 6, 3 hrs, 10 min, 5–6 sec. Queens in a control group were anaesthetised only once. The mean number of days between the second treatment and the onset of oviposition in particular groups and in the control group was 10.3, 7.6, 9.9, 10.5, 7.8, 10.9, 14.2, 15 and 21.9, respectively, and the median was 9, 7, 7, 8, 7, 8.5, 11.5, 13 and 21 days, respectively. Queens in groups 5–6 sec and 10 min began oviposition significantly earlier than those in control group, but significantly later than in groups from 3 to 96 hrs. Instrumentally inseminated queens started to lay eggs at the same time (mean 12 and median 9 days after second treatment) as queens treated with carbon dioxide without insemination (mean 11.7 and median 9 days).

Effect of Time Between Carbon Dioxide Treatments on the Onset of Oviposition in Queen Honey Bees

Apiculture Division, Institute of Animal Science

Department of Animal Genetics and Conservation, Institute of Animal Sciences

Journal of Apicultural Science

This work is licensed under the Creative Commons Attribution 4.0 International License.

It is commonly known that two carbon dioxide treatments accelerate the beginning of egg laying in honey bee queens. However, the minimum time between...

Storage conditions*	Number of queens	Min - Max	Mean ± sd	Median
Nuclei	12	6 - 29	12.5 ± 7.34	10 a
Incubator	11	6 - 35	21.7 ± 9.35	17 b
Overall	23	6 - 35	16.9 ± 9.43	14

Queens	Number of queens	Min - Max	Mean ± sd	Median
Inseminated	95	2 - 35	12.0 ± 8.87	9^a
Virgins	100	2 - 41	11.7 ± 8.48	9^a
Overall	195	2 - 41	11.9 ± 8.65	9

Time between treatments	Number of queens	Min - Max	Mean ± sd	Median
96 hrs	18	3 - 33	10.3 ± 7.84	9^a
48 hrs	22	2 - 26	7.6 ± 5.05	7^a
24 hrs	29	2 - 34	9.9 ± 7.65	7^a
12 hrs	19	2 - 30	10.5 ± 8.60	8^a
6 hrs	19	2 - 17	7.8 ± 4.47	7^a
3 hrs	22	2 - 41	10.9 ± 9.48	8.5^a
10 min*	30	4 - 35	14.2 ± 7.98	11.5^b
5–6 sec*	19	3 - 37	15.0 ± 7.87	13^b
Control**	17	6 - 41	21.9 ± 10.52	21^c
Overall	195	2 - 41	11.9 ± 8.65	9

Effect of Time Between Carbon Dioxide Treatments on the Onset of Oviposition in Queen Honey Bees

Article Category: Original Article

Publié en ligne: 27 déc. 2022

Pages: 171 - 178

Reçu: 11 avr. 2022

Accepté: 14 nov. 2022

DOI: https://doi.org/10.2478/jas-2022-0011

Mots clés
anaesthesia, carbon dioxide treatment, instrumental insemination, onset of oviposition, queen honey bee, virgin queen

© 2022 Jakub Gąbka et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Onset of oviposition by virgin queens depending on storage conditions in time between two CO2 treatments (days after treatments)

Onset of oviposition by instrumentally inseminated queens and virgins (days after second CO2 treatment)

Onset of oviposition of queens depending on time between first and second CO2 treatment (days after second treatment)

Effect of Time Between Carbon Dioxide Treatments on the Onset of Oviposition in Queen Honey Bees

Article Category: Original Article

Publié en ligne: 27 déc. 2022

Pages: 171 - 178

Reçu: 11 avr. 2022

Accepté: 14 nov. 2022

DOI: https://doi.org/10.2478/jas-2022-0011

Mots clésanaesthesia, carbon dioxide treatment, instrumental insemination, onset of oviposition, queen honey bee, virgin queen

© 2022 Jakub Gąbka et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Onset of oviposition by virgin queens depending on storage conditions in time between two CO2 treatments (days after treatments)

Onset of oviposition by instrumentally inseminated queens and virgins (days after second CO2 treatment)

Onset of oviposition of queens depending on time between first and second CO2 treatment (days after second treatment)

Mots clés
anaesthesia, carbon dioxide treatment, instrumental insemination, onset of oviposition, queen honey bee, virgin queen