The Significance of Monitoring Serum and Faecal Calprotectin in Healthy Dogs

1 Allenspach, K. A., Wieland, B., Gröne, A., Gaschen, F., 2007: Chronic enteropathies in dogs: evaluation of risk factors for negative outcome. J. Vet. Intern. Med., 21, 4, 700‒708. DOI: 10.1892/0891- 6640(2007)21[700:ceideo]2.0.co;2. Search in Google Scholar

2 Allenspach, K. A., 2015: Diagnosis of small intestinal disorders in dogs and cats. Clin. Lab. Med., 35, 521‒534. DOI: 10.1016/j.cell.2015.05.003. Search in Google Scholar

3 Allenspach, K., Culverwell, C., Chan, D., 2016: Long-term outcome in dogs with chronic enteropathies: 203 cases, Vet. Rec., 178, 15, 362‒368. DOI: 10.1136/vr.103557. Search in Google Scholar

4 Allenspach, K. A., Mochel, J. P., Du, Y., Preistnall, S. L., Moore, F., Slayter, M., et al., 2019: Correlation gastrointestinal histopathologic changes to clinical disease activity in dogs with idiopathic inflammatory bowel disease. Vet. Pathol., 56, 3, 435‒443. DOI: 10.1177/0300985818813090 Search in Google Scholar

5 Allenspach, K. A., Mochel, J. P., 2022: Current diagnostics for chronic enteropathies in dogs. Vet. Clin. Pathol., 50, Suppl. 1, 18‒28. DOI: 10.1111/vcp. 13068. Search in Google Scholar

6 Cerqutella, M., Spaterna, A., Laus, F., Tesei, B., Rossi, G., Antonelli, E., et al., 2010: Inflammatory bowel disease in the dog: Diferences and similarities with humans. World J. Gastroenterol., 16, 9, 1050‒1056. DOI: 10.3748/wjg.v16.i9.1050. Search in Google Scholar

7 Dandrieux, J. R. S., 2016: Inflammatory bowel disease versus chronic enteropathy in dogs: Are they one and the same? J. Small Anim. Pract., 57, 589‒599. DOI: 10.1111/ jsap.12588. Search in Google Scholar

8 Dandrieux, J. R. S., Mansfield, C. S., 2019: Chronic enteropathy. In Canines: Prevalence, impact and management strategies. Vet. Med. Res. Rep., 10, 203‒214. DOI: 10.2147/ VMRR.S162774. Search in Google Scholar

9 Day, M. J., Bilzer, T., Mansell, J., Wilcock, B., Hall, E. J., Jergens, A., et al., 2008: Histopathological standards for the diagnosis of gastrointestinal inflammation in endoscopic biopsy samples from the dog and cat: A report from the World Small Animal Veterinary Association Gastrointestinal Standardization Group. J. Comp. Pathol., 138, S1, 43. DOI: 10.1016/j.jcpa.2008.01.001. Search in Google Scholar

10 Dupouy-Manescau, N., Méric, T., Sénécat, O., Drut, A., Valentin, S., Oliveira, R. L., Hernandez, J., 2024: Updating the classification of chronic inflammatory enteropathies in dogs. Animals, 14, 681. DOI: 10.3390/ani14050681. Search in Google Scholar

11 Enderle, L. L., Köller, G., Heilmann, R. M., 2022: Verification of fCAL turboimmunoturbidimetric assay for measurement of faecal calprotectin concentrations in dogs and cats. J. Vet. Diagn. Invest., 34, 5, 813‒824. DOI: 10.1177/10406387221114031. Search in Google Scholar

12 Estruch, J., Johnson, J., Ford, S., Yoshimoto, S., Mills, T., Bergman, P., 2021: Utility of the combined use of 3 serologic markers in the diagnosis and monitoring of chronic enteropathies in dogs. J. Vet. Intern. Med., 35, 3, 1306‒1315. DOI: 10.1111/jvim.16132. Search in Google Scholar

13 Garcia-Sancho, M., Rodríguez-Franco, F., Sainz, A., Mancho, C., Rodríguez, A., 2007: Evaluation of clinical, macroscopic, and histopathologic response to treatment in nonhypoproteinemic dogs with lymphocytic-plasmacytic enteritis. J. Vet. Intern. Med., 21, 11‒17. DOI: 10.1892/0891-6640(2007)21[11:eocmah]2.0.co;2. Search in Google Scholar

14 Geffré, A., Friedrichs, K., Harr, K., Concordet D., Trumel, C., Braun, J. P., 2009: Reference values: A review. Vet. Clin. Pathol., 38, 288‒298. DOI: 10.1111/j.1939-165X.2009.00179.x. Search in Google Scholar

15 Grellet, A., Heilmann, R. M., Lecoindre, P., Feugier, A., Day, M. J., Peeters, D., et al., 2013: Faecal calprotectin concentrations in adult dogs with chronic diarrhoea. Am. J. Vet. Res., 74, 5, 706‒711. DOI: 10.2460/ajvr.74.5.706. Search in Google Scholar

16 Grellet, A., Mila, H., Heilmann, R. M., Feugier, A., Gruetzner, N., Suchodolski, J. S., et al., 2014: Effect of age, gestation and lactation on fecal IgA and calprotectin concentrations in dogs. Waltham Supplement, JSN, 3, 1‒5. DOI: 10.1017/jns.2014.44. Search in Google Scholar

17 Heilmann, R. M., Suchodolski, J. S., Steiner, J. M., 2008: Development and analytic validation of a radioimmunoassay for the quantification of canine calprotectin in serum and feces from dogs. Am. J. Vet. Res., 69, 845‒853. DOI: 10.2460/ ajvr.69.7.845. Search in Google Scholar

18 Heilmann, R. M., Jergens, A. E., Ackermann, M. R., Barr, J. W., Suchodolski, J. S., Steiner, J. M., 2012: Serum calprotectin concentrations in dogs with idiopathic inflammatory bowel disease. Am. J. Vet. Res., 73, 1900‒1907. DOI: 10.2460/ajvr.73.12.1900. Search in Google Scholar

19 Heilmann, R. M., Grellet, A., Allenspach, K., Lecoindre, P., Day, M. J., Priestnal, S. L., et al., 2014: Association between faecal S100A12 concentration and histologic, endoscopic, and clinical disease severity in dogs with idiopathic inflammatory bowel disease. Vet. Immunol. Immunopathol., 158, 156‒166. DOI: 10.1016/j.vetimm.2014.01.006. Search in Google Scholar

20 Heilmann, R. M., Berghoff, N., Mansell, J., Grützner, N., Parnell, N. K., Gurtner, C., et al., 2018: Association of faecal calprotectin concentrations with disease severity, response to treatment, and other biomarkers in dogs with chronic inflammatory enteropathies. J. Vet. Intern. Med., 32, 679‒692. DOI: 10.1111/jvim.15065. Search in Google Scholar

21 Heilmann, R. M., Steiner, J. M., 2018: Clinical utility of currently available biomarkers in inflammatory enteropathies of dogs. J. Vet. Intern. Med., 32,1495‒1508. DOI: 10.1111/ jvim.15247. Search in Google Scholar

22 Heilmann, R. H., Ruaux, C. G., Grützner, N., Crand-ford, S. M., Bridges, C. S., Steiner, J. M., 2019: Biological variation of serum canine calprotectin concentrations as measured by ELISA in healthy dogs. Vet. J., 247, 61‒64. DOI: 10.1016/j.tvjl.2019.03.002. Search in Google Scholar

23 Heilmann, R. M., Nestler, J., Schwarz, J., Grützner, N., Ambrus, A., Seeger, J., et al., 2019: Mucosal expression of S100A12 (calgranulin C) and S100A8/A9 (calprotectin) and corellation with serum and fecal concentrations in dogs with chronic inflammatory enteropathy. Vet. Immunol. Immunopathol., 211, 64‒74. DOI: 10.1016/j.vetimm.2019.04.003. Search in Google Scholar

24 Heilmann, R. M., Xenoulis, P., Müller, K., Stavroulaki, E. M., Suchodolski, J. S., Steiner, J. M., 2019: Association of serum calprotectin (A100A8/A9) concentrations and idiopathic hyperlipidemia in Miniature Schnauzers. J. Vet. Inter. Med., 1‒10. DOI: 10.1111/jvim.15460. Search in Google Scholar

25 Hernandez, J., Rouillé, E., Chocteau, F., Allard, M., Haurogné, K., Hervé, J. M., et al., 2021: Nonhypoalbuminemic inflammatory bowel disease in dogs as disease model. Inflam. Bowel Dis., 27, 1975‒1985. DOI: 10.1093/ibd/izab064. Search in Google Scholar

26 Jergens, A. E., Schneider, C. A., Frank, D. E., 2003: A scoring index for disease activity in canine inflammatory bowel disease. J. Vet. Med., 17, 291‒297. DOI: 10.1111/j.1939-1676.2003.tb02450.x Search in Google Scholar

27 Jergens, A. E., Heilmann, R. M., 2022: Canine chronic enteropathy-current astate-of-the-art and emerging concepts. Front. Vet. Sci., 9, 923013. DOI: 10.3389/fvets.2022.923013. Search in Google Scholar

28 Kalenyak, K., Isaiah, A., Heilmann, R. M., Suchodolski, J. S., Burgener, I. A., 2018: Comparison of the intestinal mucosal microbiota in dogs diagnosed with idiopathic inflammatory bowel disease and dogs with food-responsive diarrhoea before and after treatment. FEMS Microbiol. Ecol., 94, 2, 1‒11. DOI: 10.1093/femsec/fix173. Search in Google Scholar

29 Kathrani, A., Werling, D., Allenspach, K., 2011: Canine breeds at high risk of developing inflammatory bowel disease in the south-eastern area UK. Vet. Rec., 10, 169, 635. DOI: 10.1136/vr.d5380. Search in Google Scholar

30 Kobayashi, S., Ohno, K., Uetsuka, K., Nakashima, K., Setoguchi, A., Fujino, Y., Tsujimoto, H., 2007: Measurement of intestinal mucosal permeability in dogs with lymphocytic-plasmacytic enteritis. J. Vet. Med. Sci., 69, 745‒749. DOI: 10.1292/jvms.69.745. Search in Google Scholar

31 Langlois, D. K., Pritchard, J. C., Tolbert, M. K., Jergens, A. E., Block, G., Hanzlicek, A., et al., 2023: Clinical utility of immunoglobulin A-based serological panel for the diagnosis of chronic enteropathy in dogs. J. Vet. Intern. Med., 37, 446‒454. DOI: 10.1111/jvim.16636. Search in Google Scholar

32 Nestler, J., Syrjä, R., Kilpinen, S., Moniz, C. A., Spill-mann, T., Hanifeh, M., et al., 2024: Duodenal and colonic mucosal S100A8/A9 (calprotectin) expression is increased and correlates with the severity of select histologic lesions in dogs with chronic inflammatory enteropathy. BMC Vet. Res., 20, 393. DOI: 10.1186/s12917-024-04256-9. Search in Google Scholar

33 O’Neill, D. G., James, H., Brodbelt, D. C., Church, D. B., Pegram, C., 2021: Prevalence of commonly diagnosed disorders in UK dogs under primary veterinary care: Results and applications. BMC Vet. Res., 17, 69. DOI: 10.1186/s12917-021-02775-3. Search in Google Scholar

34 Otoni, C. C., Heilmann, R. M., Garcia-Sancho, M., Sainz, A., Ackermann, M. R., Suchodolski, J. S., et al., 2018: Serologic and faecal markers to predict response to induction therapy in dogs with idiopathic inflammatory bowel disease. J. Vet. Intern. Med., 32, 999‒1008. DOI: 10.1111/jvim.15123. Search in Google Scholar

35 Pilla, R., Suchodolski, J. S., 2020: The role of the canine gut microbiome and metabolome in health and gastrointestinal disease. Front. Vet. Sci., 6, 498. DOI: 10.3389/ fvets.2019.00498. Search in Google Scholar

36 Sacoor, C., Barros, L. M., Montezinho, L., 2020: What are the potential biomarkers that should be considered in diagnosing and managing canine chronic inflammatory enter-opathies? Open Vet. J., 10, 4, 412‒430. DOI: 10.4314/ovj. v10i4.9. Search in Google Scholar

37 Simpson, K. W., Jergens, A. E., 2011: Pitfalls and progress in the diagnosis and management of canine inflammatory bowel disease. Vet. Clin. Small Anim., 41, 381‒398. DOI: 10.1016/j.cvsm.2011.02.003. Search in Google Scholar

38 Suchodolski, J. S. Markel, M. E., Garcia-Mazcorro, J. F., Unterer, S., Heilmann, R. M., Dowd, S. E., et al., 2012: The faecal microbiome in dogs with acute diarrhoea and idiopathic inflammatory bowel disease. PLOS ONE, 7, 12, e51907 DOI: 10.1371/journal.pone.0051907. Search in Google Scholar

39 Suchodolski, J. S., 2016: Diagnosis and interpretation of intestinal dysbiosis in dogs and cats. Vet. J., 215, 30‒37. DOI: 10.1016/j.tvjl.2016.04.011. Search in Google Scholar

40 Volkmann, M., Steiner, J. M., Fosgate, G. T., Zentek, J., Hartmann, S., Kohn, B., 2017: Chronic diarrhoea in dogs ‒ retrospective study in 136 cases. J. Vet. Intern. Med., 31, 1043‒1055. DOI: 10.1111/jvim.14739. Search in Google Scholar

41 Weber, M. P., Martin, L. J., Dumon, H. J., Giorge, V. C., Nguyen, P. G., 2002: Influence of age and body size on intestinal permeability and absorption in healthy dogs. Am. J. Vet. Res., 63, 1323‒1328. DOI: 10.2460/ajvr.2002.63.1323. Search in Google Scholar