<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The aim of this study was to evaluate the toxigenic potential of <italic>Bacillus cereus</italic> strains isolated from frozen goat colostrum. Of the 50 phenotypically suspected <italic>B. cereus</italic> isolates, 39 (78.0 %) were confirmed as <italic>B. cereus</italic> by the polymerase chain reaction (PCR) method based on the gyrB gene detection. In these isolates, genes encoding the production of haemolysin BL (Hbl), a complex of non-haemolytic enterotoxins (Nhe) and emetic toxin were detected by the PCR method. In 36 (92.3 %) confirmed <italic>B. cereus</italic> isolates, genes encoding at least one type of toxins of interest were detected. In all toxigenic isolates, we found the presence of genes for Nhe production, and in 16 (41.0 %) of the isolates, genes encoding both Nhe and haemolysin BL were shown. Eight (20.5 %) of the emetic strains of <italic>B. cereus</italic> were identified. The emetic toxin production gene was always detected simultaneously with genes encoding non-haemolytic enterotoxin production. The ability to produce BL haemolysin and non-haemolytic enterotoxins were confirmed by the immunochromatographic method. In summary, goat colostrum can be a significant source of toxigenic strains of <italic>B. cereus</italic>.</p></abstract>

The aim of this study was to evaluate the toxigenic potential of Bacillus cereus strains isolated from frozen goat colostrum. Of the 50 phenotypically suspected B. cereus isolates, 39 (78.0 %) were confirmed as B. cereus by the polymerase chain reaction (PCR) method based on the gyrB gene detection. In these isolates, genes encoding the production of haemolysin BL (Hbl), a complex of non-haemolytic enterotoxins (Nhe) and emetic toxin were detected by the PCR method. In 36 (92.3 %) confirmed B. cereus isolates, genes encoding at least one type of toxins of interest were detected. In all toxigenic isolates, we found the presence of genes for Nhe production, and in 16 (41.0 %) of the isolates, genes encoding both Nhe and haemolysin BL were shown. Eight (20.5 %) of the emetic strains of B. cereus were identified. The emetic toxin production gene was always detected simultaneously with genes encoding non-haemolytic enterotoxin production. The ability to produce BL haemolysin and non-haemolytic enterotoxins were confirmed by the immunochromatographic method. In summary, goat colostrum can be a significant source of toxigenic strains of B. cereus.

Goat Colostrum—Source of Toxigenic <italic>Bacillus Cereus</italic>

Department of Milk Hygiene and Technology

University of Veterinary and Pharmaceutical Sciences Brno

Department of Gastronomy, Faculty of Veterinary Hygiene and Ecology

Folia Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

{"article-title":{"italic":"Bacillus Cereus","content":"Goat Colostrum\u2014Source of Toxigenic"}}

The aim of this study was to evaluate the toxigenic potential of Bacillus cereus strains isolated from frozen goat colostrum. Of the 50 phenotypically...

Goat Colostrum—Source of Toxigenic Bacillus Cereus

Published Online: Sep 28, 2019

Page range: 27 - 33

Received: May 24, 2019

Accepted: Jul 15, 2019

DOI: https://doi.org/10.2478/fv-2019-0024

Keywords
cereulide, haemolysin BL, non-haemolytic enterotoxin, PCR

© 2019 Š. Bursová et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Goat Colostrum—Source of Toxigenic Bacillus Cereus

Published Online: Sep 28, 2019

Page range: 27 - 33

Received: May 24, 2019

Accepted: Jul 15, 2019

DOI: https://doi.org/10.2478/fv-2019-0024

Keywordscereulide, haemolysin BL, non-haemolytic enterotoxin, PCR

© 2019 Š. Bursová et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
cereulide, haemolysin BL, non-haemolytic enterotoxin, PCR