1. bookVolume 63 (2019): Issue 3 (September 2019)
Journal Details
License
Format
Journal
eISSN
2453-7837
First Published
30 Mar 2016
Publication timeframe
4 times per year
Languages
English
access type Open Access

Appraisal of the Antioxidative Potential of Aloe Barbadensis M. on Alcohol-Induced Oxidative Stress

Published Online: 28 Sep 2019
Volume & Issue: Volume 63 (2019) - Issue 3 (September 2019)
Page range: 34 - 46
Received: 27 Apr 2019
Accepted: 15 Jul 2019
Journal Details
License
Format
Journal
eISSN
2453-7837
First Published
30 Mar 2016
Publication timeframe
4 times per year
Languages
English
Abstract

This investigation estimated the anti-oxidative potential of Aloe barbadensis gel extracts in rats against alcohol-induced oxidative stress. Thirty male albino rats (5 each per group) were included in the experiments. Group A (positive control) and B (negative control) were administered 4 mg.kg–1 body weight distilled water and 50 % alcohol respectively for 21 days. Groups C and D were administered 50 % alcohol for the first 14 days followed by co-administration of 125 mg and 250 mg.kg−1 body weight extract with alcohol respectively for the last 7 days. Groups E and F were administered distilled water for the first 14 days followed by co-administration of 125 and 250 mg.kg−1 body weight Aloe barbadensis gel extracts with distilled water respectively for the last 7 days. The administration of alcohol resulted in a significant (P < 0.05) decrease in the specific activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and reduced glutathione (GSH) levels, while cholesterol (CHO), triglycerides (TAG), nitric oxide (NO) and malondialdehyde (MDA) concentrations were significantly increased when compared to the controls. Co-mobilization with Aloe barbadensis gel extracts for 7 days significantly reversed the deleterious effects of alcohol in the treated groups when compared to the alcohol group. This study indicated that Aloe barbadensis probably possesses anti-oxidative effects against alcohol–induced oxidative stress in rats.

Keywords

1. Al-Shinnawy, M. S., Hassan, A. R., Ismail, D. A., Shahin, M. A., 2014: The potential protective and therapeutic effects of aloe vera juice on malathion induced hepatotoxicity in rabbits. The Egyptian Journal of Hospital Medicine, 55, 146—158. DOI: 10.12816/000450010.12816/0004500Search in Google Scholar

2. Awazawa, M., Ueki, K., Inabe, K., Yamauchi, T., Kaneko, K., Okazaki, Y., et al., 2009: Adiponectin suppresses hepatic SREBP1c expression in an AdipoR1/LKB1/AMPK dependent pathway. Biochemical and Biophysical Research Communications, 382, 1, 51—56. DOI: 10.1016/j.bbrc.2009.02.131.10.1016/j.bbrc.2009.02.131Open DOISearch in Google Scholar

3. Bashir, A., Saeed, B., Mujahid, T. Y., Jehan, N., 2011: Comparative study of antimicrobial activities of Aloe vera extracts and antibiotics against isolates from skin infections. African Journal of Biotechnology, 10, 19, 3835—3840. DOI: 10.5897/AJB07.572.10.5897/AJB07.572Open DOISearch in Google Scholar

4. Brown, L. A. S., Harris, F. L., Ping, X D., Gauthier, T. W., 2004: Chronic ethanol ingestion and the risk of acute lung injury: a role for glutathione availability ? Alcohol, 33, 3, 191—197. DOI: 10.1016/j.alcohol.2004.08.002.10.1016/j.alcohol.2004.08.002Open DOISearch in Google Scholar

5. Calberg, I., Mannervick, B., 1985: Glutathione reductase. Method Enzymol., 113, 485—490.10.1016/S0076-6879(85)13062-4Search in Google Scholar

6. Carney, J. M., Starke-Reed, P. E., Oliver, C. N., Landum, R. W., Cheng, M. S., Wu, J. F., Floyd, R. A., 1991: Reversal of age-related increase in brain protein oxidation, decrease in enzyme activity, and loss in temporal and spatial memory by chronic administration of the spin-trapping compound N-tert-butyl-alpha-phenylnitrone. Proceedings of the National Academy of Sciences, 88, 9, 3633—3636. DOI: 10.1073/pnas.88.9.3633.10.1073/pnas.88.9.3633515061673789Open DOISearch in Google Scholar

7. Chandan, B. K., Saxena, A. K., Shukla, S., Sharma, N., Gupta, D. K., Suri, K. A., et al., 2007: Hepatoprotective potential of Aloe barbadensis Mill. against carbon tetrachloride induced hepatotoxicity. Journal of Ethnopharmacology, 111, 3, 560—566. DOI: 10.1016/j.jep.2007.01.008.10.1016/j.jep.2007.01.00817291700Open DOISearch in Google Scholar

8. Chen, L., Wang, F., Sun, X., Zhou, J., Gao, L., Jiao, Y., et al., 2010: Chronic ethanol feeding impairs AMPK and MEF2 expression and is associated with GLUT4 decrease in rat myocardium. Experimental and Molecular Medicine, 42, 3, 205—215. DOI: 10.3858/emm.2010.42.3.021.10.3858/emm.2010.42.3.021284500520164678Search in Google Scholar

9. Chinedu, E., Arome, D., Ameh, F. S., 2013: A new method for determining acute toxicity in animal models. Toxicology International, 20, 3, 224—226. DOI: 10.4103/0971-6580.121674.10.4103/0971-6580.121674387749024403732Open DOISearch in Google Scholar

10. Chun-hui, L., Chang-hai, W., Zhi-liang, X., Yi, W., 2007: Isolation, chemical characterization and antioxidant activities of two polysaccharides from the gel and the skin of Aloe barbadensis Miller irrigated with sea water. Process Biochemistry, 42, 6, 961—970. DOI: 10.1016/j.procbio.2007.03.004.10.1016/j.procbio.2007.03.004Search in Google Scholar

11. Cui, Y., Ye, Q., Wang, H., Li, Y., Yao, W. Qian, H., 2014: Hepatoprotective potential of Aloe vera polysaccharides against chronic alcohol-induced hepatotoxicity in mice. Journal of the Science of Food and Agriculture, 94, 9, 1764—1771. DOI: 10.1002/jsfa.6489.10.1002/jsfa.6489Open DOISearch in Google Scholar

12. Debnath, T., Ghosh, M., Lee, Y. M., Nath, N. C. D., Lee, K. G., Lim, B. O., 2018: Identification of phenolic constituents and antioxidant activity of Aloe barbadensis flower extracts. Food and Agricultural Immunology, 29, 1, 27—38. DOI:10.1080/09540105.2017.1358254.10.1080/09540105.2017.1358254Open DOISearch in Google Scholar

13. Deleve, L. D., Kaplowitz, N., 1990: November. Importance and regulation of hepatic glutathione. In Seminars in Liver Disease, Thieme Medical Publishers, Inc., 10, 4, 251—266.10.1055/s-2008-1040481Search in Google Scholar

14. Donohue Jr., T. M., 2007: Alcohol-induced steatosis in liver cells. World Journal of Gastroenterology, 13, 37, 4974—4978. DOI: 10.3748/wjg.v13.i37.4974.10.3748/wjg.v13.i37.4974Open DOISearch in Google Scholar

15. Dosumu, O. O., Osinubi, A. A. A., Duru, F. I. O., 2014: Alcohol induced testicular damage: Can abstinence equal recovery ? Middle East Fertility Society Journal, 19, 3, 221—228. DOI: 10.1016/j.mefs.2014.01.003.10.1016/j.mefs.2014.01.003Open DOISearch in Google Scholar

16. Dupont, I., Bodenez, P., Berthou, F., Simon, B., Bardou, L. G., Lucas, D., 2000: Cytochrome P-450 2E1 activity and oxidative stress in alcoholic patients. Alcohol and Alcoholism, 35, 1, 98—103. DOI: 10.1093/alcalc/35.1.98.10.1093/alcalc/35.1.98Open DOISearch in Google Scholar

17. Fraga, C. G., Leibovitz, B. E., Tappel, A. L., 1988: Lipid peroxidation measured as thiobarbituric acid-reactive substances in tissue slices: characterization and comparison with homogenates and microsomes. Free Radical Biology and Medicine, 4, 3, 155—161. DOI: 10.1016/0891-5849(88)90023-8.10.1016/0891-5849(88)90023-8Open DOISearch in Google Scholar

18. Gardner, C. D., Coulston, A., Chatterjee, L., Rigby, A., Spiller, G., Farquhar, J. W., 2005: The effect of a plant-based diet on plasma lipids in hypercholesterolemic adults: a randomized trial. Annals of Internal Medicine, 142, 9, 725—733. DOI: 10.7326/0003-4819-142-9-200505030-00007.10.7326/0003-4819-142-9-200505030-00007Open DOISearch in Google Scholar

19. Gidez, L. I., Miller, G. J., Burstein, M., Slagle, S., Eder, H. A., 1982: Separation and quantitation of subclasses of human plasma high density lipoproteins by a simple precipitation procedure. Journal of Lipid Research, 23, 8, 1206—1223.10.1016/S0022-2275(20)38059-7Search in Google Scholar

20. Ginsberg, H. N., 1994: Lipoprotein metabolism and its relationship to atherosclerosis. The Medical Clinics of North America, 78, 1, 1—20. DOI: 10.1016/S0025-7125(16)30174-2.10.1016/S0025-7125(16)30174-2Open DOISearch in Google Scholar

21. Glew, R. H., Kassam, H. A., Bhanji, R. A., Okorodudu, A., VanderJagt, D. J., 2002: Serum lipid profiles and risk of cardiovascular disease in three different male populations in northern Nigeria. Journal of Health, Population and Nutrition, 20, 166—174.Search in Google Scholar

22. Hadwan, M. H., Abed, H. N., 2016: Data supporting the spec-trophotometric method for the estimation of catalase activity. Data in Brief, 6, 194—199. DOI: 10.1016/j.dib.2015.12.012.10.1016/j.dib.2015.12.012Open DOISearch in Google Scholar

23. Halliwell, B., 1987: Oxidants and human disease: some new concepts. The FASEB Journal, 1, 5, 358—364. DOI: 10.1096/fasebj.1.5.2824268.10.1096/fasebj.1.5.2824268Open DOISearch in Google Scholar

24. Hu, S., Yin, S., Jiang, X., Huang, D., Shen, G., 2009: Melatonin protects against alcoholic liver injury by attenuating oxidative stress, inflammatory response, and apoptosis. European Journal of Pharmacology, 616, 1—3, 287—292. DOI: 10.1016/j.ejphar.2009.06.044.10.1016/j.ejphar.2009.06.044Open DOISearch in Google Scholar

25. Johnsen-Soriano, S., Genovés, J. M., Romero, B., García-Delpech, S., Muriach, M., Sancho-Tello, M., et al., 2007: Chronic ethanol feeding induces oxidative stress in the rat retina: treatment with the antioxidant ebselen. Archivos de la Sociedad Espanola de Oftalmologia, 82, 12, 757—762.10.4321/S0365-66912007001200008Search in Google Scholar

26. Josias, H. H, 2008: Composition and applications of Aloe vera leaf gel. Molecules, 13, 8, 1599—1616. DOI: 10.3390/molecules13081599.10.3390/molecules13081599Search in Google Scholar

27. Kukielka, E., Dicker, E., Cederbaum, A. I., 1994: Increased production of reactive oxygen species by rat liver mitochondria after chronic ethanol treatment. Archives of Biochemistry and Biophysics, 309, 2, 377—386. DOI: 10.1006/abbi.1994.1127.10.1006/abbi.1994.1127Open DOISearch in Google Scholar

28. Lakshmi, P. T. V., Rajalakshmi, P., 2011: Identification of phyto components and its biological activities of Aloe vera through the gas chromatography-mass spectrometry. Int. Res. J. Pharm., 2, 5, 247—249.Search in Google Scholar

29. Lampe, M. A., Burlingame, A. L., Whitney, J., Williams, M. L., Brown, B. E., Roitman, E., Elias, P. M., 1983: Human stratum corneum lipids: characterization and regional variations. J. Lipid Res., 24, 2, 120—130.10.1016/S0022-2275(20)38005-6Search in Google Scholar

30. Lee, H. I., McGregor, R. A., Choi, M. S., Seo, K. I., Jung, U. J., Yeo, J., et al., 2013: Low doses of curcumin protect alcohol-induced liver damage by modulation of the alcohol metabolic pathway, CYP2E1 and AMPK. Life Sciences, 93, 18—19, 693—699. DOI: 10.1016/j.lfs.2013.09.014.10.1016/j.lfs.2013.09.01424063989Open DOISearch in Google Scholar

31. Li, S. Y., Gomelsky, M., Duan, J., Zhang, Z., Gomelsky, L., Zhang, X., Epstein, P. N., Ren, J., 2004: Overexpression of aldehyde dehydrogenase-2 (ALDH2) transgene prevents acetaldehyde-induced cell injury in human umbilical vein endothelial cells role of ERK and p38 mitogen-activated protein kinase. Journal of Biological Chemistry, 279, 12, 11244—11252. DOI: 10.1074/jbc.M308011200.10.1074/jbc.308011200Open DOISearch in Google Scholar

32. Lichtenstein, A. H., Appel, L. J., Brands, M., Carnethon, M., Daniels, S., Franch, H. A., et al., N., 2006: Diet and lifestyle recommendations revision 2006: a scientific statement from the American Heart Association Nutrition Committee. Circulation, 114, 1, 82—96. DOI: 10.1161/CIRCULATIONAHA.106.176158.10.1161/CIRCULATIONAHA.106.17615816785338Open DOISearch in Google Scholar

33. Lieber, C. S., 2004: Alcoholic fatty liver: its pathogenesis and mechanism of progression to inflammation and fibrosis. Alcohol, 34, 1, 9—19. DOI: 10.1016/j.alcohol.2004.07.008.10.1016/j.alcohol.2004.07.008Open DOISearch in Google Scholar

34. Marklund, S., Marklund, G., 1974: Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem., 47, 3, 469—474. DOI: 10.1111/j.1432-1033.1974.tb03714.x.10.1111/j.1432-1033.1974.tb03714.xOpen DOISearch in Google Scholar

35. McKim, S. E., Gäbele, E., Isayama, F., Lambert, J. C., Tucker, L. M., Wheeler, M. D., et al., 2003: Inducible nitric oxide synthase is required in alcohol-induced liver injury: studies with knockout mice. Gastroenterology, 125, 6, 1834—1844. DOI: 10.1053/j.gastro.2003.08.030.10.1053/j.gastro.2003.08.030Open DOISearch in Google Scholar

36. Moron, M. S., Depierre, J. W., Mannervik, B., 1979: Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochimica et Biophysica Acta (BBA)-General Subjects, 582, 1, 67—78. DOI: 10.1016/0304-4165(79)90289-7.10.1016/0304-4165(79)90289-7Open DOISearch in Google Scholar

37. National Research Council 2006: Guide for the Care and Use of Laboratory Animals, 8th edn., The National Academic Press, Washington DC, 41—103.Search in Google Scholar

38. Naveena, Bharath, B. K., Selvasubramanyam, 2011: Antitumor activity of Aloe vera against Ehrlich ascitis carcinoma in Swiss albino mice. International Journal of Pharma and Bioscience, 2, 2, 400—409.Search in Google Scholar

39. Nguyen, K. H., Lee, J. H., Nyomba, B. G., 2012: Ethanol causes endoplasmic reticulum stress and impairment of insulin secretion in pancreatic β-cells. Alcohol, 46, 1, 89—99. DOI: 10.1016/j.alcohol.2011.04.001.10.1016/j.alcohol.2011.04.00121840159Open DOISearch in Google Scholar

40. Padmanabhan, P., Jangle, S. N., 2014: Hepatoprotective activity of herbal preparation (HP-4) against alcohol induced hepatotoxicity in mice. Int. J. Appl. Sci. Biotech., 2, 1, 50—58. DOI: 10.3126/ijasbt.v2i1.9346.10.3126/ijasbt.v2i1.9346Open DOISearch in Google Scholar

41. Pal, S., Bhattacharjee, A., Mukherjee, S., Bhattacharya, K., Mukherjee, S., Khowala, S., 2014: Effect of Alocasia indica tuber extract on reducing hepatotoxicity and liver apoptosis in alcohol intoxicated rats. BioMed Res. Int., 2014, 10 pp. DOI: 10.1155/2014/349074.10.1155/2014/349074405814024977149Search in Google Scholar

42. Pandanaboina, S. C., Kondeti, S. R., Rajbanshi, S. L., Kunala, P. N., Pandanaboina, S., Pandanaboina, M. M., Wudayagiri, R., 2012: Alterations in antioxidant enzyme activities and oxidative damage in alcoholic rat tissues: Protective role of Thespesia populnea. Food Chemistry, 132, 1, 150—159. DOI: 10.1016/j.foodchem.2011.10.046.10.1016/j.foodchem.2011.10.04626434274Open DOISearch in Google Scholar

43. Rotruck, J. T., Pope, A. L., Ganther, H. E., Swanson, A. B., Hafeman, D. G. Hoekstra, W., 1973: Selenium: biochemical role as a component of glutathione peroxidase. Science, 179, 4073, 588—590. DOI: 10.1126/science.179.4073.588.10.1126/.179.4073.588Open DOISearch in Google Scholar

44. Saljooghianpour, M., Javaran, T. A., 2013: Identification of phytochemical components of aloe plantlets by gas chromatography-mass spectrometry. African Journal of Biotechnology, 12, 49, 6876—6880. DOI: 10.5897/AJB11.4307.10.5897/AJB11.4307Open DOISearch in Google Scholar

45. Sathyaprabha, G., Kumaravel, S., Ruffina, D., Praveenkumar, P., 2010: A comparative study on antioxidant, proximate analysis, antimicrobial activity and phytochemical analysis of Aloe vera and Cissus quadrangularis by GC-MS. Journal of Pharmacy Research, 3, 12, 2970—2973.Search in Google Scholar

46. Scott, R. B., Reddy, K. S., Husain, K., Schlorff, E. C., Rybak, L. P., Somani, S. M., 2000: Dose response of ethanol on anti-oxidant defense system of liver, lung, and kidney in rat. Patho-physiology, 7, 1, 25—32. DOI: 10.1016/S0928-4680(99)00034-6.10.1016/S0928-4680(99)00034-6Open DOISearch in Google Scholar

47. Sild, E., Hõrak, P., 2009: Nitric oxide production: an easily measurable condition index for vertebrates. Behavioral Ecology and Sociobiology, 63, 6, 959—966. DOI: 10.1007/s00265-009-0710-0.10.1007/s00265-009-0710-0Open DOISearch in Google Scholar

48. Szabo, G., Bala, S., 2010: Alcoholic liver disease and the gut-liver axis. World Journal of Gastroenterology: WJG, 16, 11, 1321—1329. DOI: 10.3748/wjg.v16.i11.1321.10.3748/wjg.v16.i11.1321Open DOISearch in Google Scholar

49. Tajoda, H. N., Kurian, J. C., Bredenkamp, M. B., 2013: Reduction of cholesterol and triglycerides in volunteers using lemon and apple. Int. J. Humanit. Soc. Sci., 3, 18, 60—64.Search in Google Scholar

50. Van der Gaag, M. S., Van Tol, A., Vermunt, S. H. F., Scheek, L. M., Schaafsma, G., Hendriks, H. F. J., 2001: Alcohol consumption stimulates early steps in reverse cholesterol transport. Journal of Lipid Research, 42, 12, 2077—2083.10.1016/S0022-2275(20)31537-6Search in Google Scholar

51. Vogt, B. L., Richie Jr., J. P., 2007: Glutathione depletion and recovery after acute ethanol administration in the aging mouse. Biochemical Pharmacology, 73, 10, 1613—1621. DOI: 10.1016/j.bcp.2007.01.03310.1016/j.bcp.2007.01.033193016217343832Search in Google Scholar

52. Zima, T., Fialová, L., Mestek, O., Janebová, M., Crkovská, J., Malbohan, I., et al., 2001: Oxidative stress, metabolism of ethanol and alcohol-related diseases. Journal of Biomedical Science, 8, 1, 59—70. DOI: 10.1007/BF02255972.10.1007/BF0225597211173977Open DOISearch in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo