<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Different chemicals can have genotoxic effects on the body, as confirmed by chromosome damage detection. Using conventional cytogenetic analysis and fluorescence in situ hybridization, we tested the extent of chromosome damage caused by the acetamiprid-based insecticide Mospilan 20SP on bovine peripheral blood lymphocytes at concentrations of, 2.5, 5, 25 and 50 µg.ml<sup>−1</sup> after a 24 h incubation period. During the experiment, the presence of unstable aberrations—chromosomal and chromatid breaks and gaps—were detected by conventional cyto-genetic analysis. With increasing insecticide concentrations, we observed a statistically significant increase in chromosome damage frequency after 24 hours of exposure. Fluorescence in situ hybridization was used to detect stable structural aberrations; whole-chromosome painting probes for bovine chromosomes 1 and 7 (BTA 1 and BTA 7) were used for this purpose. As a result of exposure to the insecticide, neither BTA 1/BTA 7 translocations nor other types of translocations were observed.</p></abstract>

Different chemicals can have genotoxic effects on the body, as confirmed by chromosome damage detection. Using conventional cytogenetic analysis and fluorescence in situ hybridization, we tested the extent of chromosome damage caused by the acetamiprid-based insecticide Mospilan 20SP on bovine peripheral blood lymphocytes at concentrations of, 2.5, 5, 25 and 50 µg.ml−1 after a 24 h incubation period. During the experiment, the presence of unstable aberrations—chromosomal and chromatid breaks and gaps—were detected by conventional cyto-genetic analysis. With increasing insecticide concentrations, we observed a statistically significant increase in chromosome damage frequency after 24 hours of exposure. Fluorescence in situ hybridization was used to detect stable structural aberrations; whole-chromosome painting probes for bovine chromosomes 1 and 7 (BTA 1 and BTA 7) were used for this purpose. As a result of exposure to the insecticide, neither BTA 1/BTA 7 translocations nor other types of translocations were observed.

Analysis of Chromosomal Damage Caused by Acetamiprid

Department of Biology and Genetics, Institute of Genetics

University of Veterinary Medicine and Pharmacy

Folia Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

{"article-title":"Analysis of Chromosomal Damage Caused by Acetamiprid"}

Different chemicals can have genotoxic effects on the body, as confirmed by chromosome damage detection. Using conventional cytogenetic analysis and...

Analysis of Chromosomal Damage Caused by Acetamiprid

Published Online: Jul 09, 2019

Page range: 25 - 29

Received: Feb 15, 2019

Accepted: May 29, 2019

DOI: https://doi.org/10.2478/fv-2019-0015

Keywords
acetamiprid, conventional cytogenetic analysis, chromosomal aberrations, fluorescence in situ hybridization

© 2019 Stupáková K. et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Analysis of Chromosomal Damage Caused by Acetamiprid

Published Online: Jul 09, 2019

Page range: 25 - 29

Received: Feb 15, 2019

Accepted: May 29, 2019

DOI: https://doi.org/10.2478/fv-2019-0015

Keywordsacetamiprid, conventional cytogenetic analysis, chromosomal aberrations, fluorescence in situ hybridization

© 2019 Stupáková K. et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
acetamiprid, conventional cytogenetic analysis, chromosomal aberrations, fluorescence in situ hybridization