<abstract xmlns="http://www.w3.org/1999/xhtml">Acetamiprid, that is known as the commercial formulation Mospilan® 20SP is the part of the neonicotinoid insecticide group and is widely used against various pests. In our study we assessed the potential clastogenic effects of Mospilan® in human peripheral blood lymphocytes <italic>in vitro</italic> using a chromosome aberration test. The lymphocytes were treated with acetamiprid in the concentration range of 5, 10, 25 and 50 µg.ml−1 for 24 and 48 h. After 24 h exposure, the insecticide induced statistically significant higher levels of chromosome aberrations from the concentration of 10 µg.ml−1 (P &lt; 0.05 and P &lt; 0.001) and a significant decrease in mitotic index (MI) at the concentrations of 25 and 50 µg.ml−1 (P &lt; 0.05 and P &lt; 0.01), respectively. After a 48 h exposure, we found a dose dependent increase in the percentage of chromosome aberrations at all concentrations (P &lt; 0.05; P &lt; 0.01 and P &lt; 0.001) and a decrease in MI at concentrations of 25 and 50 µg.ml−1 (P &lt; 0.05 and P &lt; 0.01). Our results indicated that neonicotinoid insecticide formulations containing acetamiprid may have potential cytotoxic and genotoxic effects.</abstract>

Acetamiprid, that is known as the commercial formulation Mospilan® 20SP is the part of the neonicotinoid insecticide group and is widely used against various pests. In our study we assessed the potential clastogenic effects of Mospilan® in human peripheral blood lymphocytes in vitro using a chromosome aberration test. The lymphocytes were treated with acetamiprid in the concentration range of 5, 10, 25 and 50 µg.ml−1 for 24 and 48 h. After 24 h exposure, the insecticide induced statistically significant higher levels of chromosome aberrations from the concentration of 10 µg.ml−1 (P < 0.05 and P < 0.001) and a significant decrease in mitotic index (MI) at the concentrations of 25 and 50 µg.ml−1 (P < 0.05 and P < 0.01), respectively. After a 48 h exposure, we found a dose dependent increase in the percentage of chromosome aberrations at all concentrations (P < 0.05; P < 0.01 and P < 0.001) and a decrease in MI at concentrations of 25 and 50 µg.ml−1 (P < 0.05 and P < 0.01). Our results indicated that neonicotinoid insecticide formulations containing acetamiprid may have potential cytotoxic and genotoxic effects.

Assessment of Potential Clastogenic Effect of the Insecticide Mospilan® 20SP in Human Peripheral Blood Lymphocytes After <italic>in Vitro</italic> Exposure

Department of Biology and Genetics, Institute of Genetics

University of Veterinary Medicine and Pharmacy in Kosice

Folia Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Assessment of Potential Clastogenic Effect of the Insecticide Mospilan 20SP in Human Peripheral Blood Lymphocytes After  Exposure

Acetamiprid, that is known as the commercial formulation Mospilan® 20SP is the part of the neonicotinoid insecticide group and is widely used against various...

Assessment of Potential Clastogenic Effect of the Insecticide Mospilan^® 20SP in Human Peripheral Blood Lymphocytes After in Vitro Exposure

Published Online: Jul 09, 2019

Page range: 21 - 24

Received: Feb 12, 2019

Accepted: May 27, 2019

DOI: https://doi.org/10.2478/fv-2019-0014

Keywords
acetamiprid, chromosome aberrations, mitotic index, neonicotinoids

© 2019 Ničová K. et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Assessment of Potential Clastogenic Effect of the Insecticide Mospilan® 20SP in Human Peripheral Blood Lymphocytes After in Vitro Exposure

Published Online: Jul 09, 2019

Page range: 21 - 24

Received: Feb 12, 2019

Accepted: May 27, 2019

DOI: https://doi.org/10.2478/fv-2019-0014

Keywordsacetamiprid, chromosome aberrations, mitotic index, neonicotinoids

© 2019 Ničová K. et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Assessment of Potential Clastogenic Effect of the Insecticide Mospilan^® 20SP in Human Peripheral Blood Lymphocytes After in Vitro Exposure

Keywords
acetamiprid, chromosome aberrations, mitotic index, neonicotinoids