Phytochemicals from  L. leaves and fruit extracted with various solvents: Their identification and quantification by LC-MS and antihyperglycemic effects

Ebru Aydin

Open Access

Phytochemicals from Phillyrea latifolia L. leaves and fruit extracted with various solvents: Their identification and quantification by LC-MS and antihyperglycemic effects

Ebru Aydin

| Jun 26, 2023

Folia Horticulturae

Volume 35 (2023): Issue 1 (June 2023)

About this article

Cite

Published Online: Jun 26, 2023

Page range: 233 - 242

Received: Feb 15, 2023

Accepted: Jun 12, 2023

DOI: https://doi.org/10.2478/fhort-2023-0018

Keywords
antidiabetic, antioxidant, LC-MS, mock privet, phytochemicals

© 2023 Ebru Aydin, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Antioxidant, α-amylase and α-glucosidase activities (IC50 mg · mL–1) for mock privet leaf and fruit extracts and acarbose 1 mg · mL–1.

Functional properties	DPPH		α-amylase		α-glucosidase
Functional properties	Leaves	Fruit	Leaves	Fruit	Leaves	Fruit
Ethyl acetate	19.3 ± 4.21 x	23.2 ± 1.92 x	2.6 ± 0.07 x	3.1 ± 0.08 x	1.8 ± 0.14 x	2.3 ± 1.02 x
Methanol	12.9 ± 2.13 x	15.1 ± 1.27 x	2.8 ± 1.39 x	4.1 ± 1.59 y	2.4 ± 1.17 y	3.0 ± 1.24 y
Water	8.6 ± 0.94 x	10.3 ± 1.31 x	11.3 ± 0.94 z	13.4 ± 1.41 z	10.9 ± 0.14 z	12.1 ± 3.15 z
Acarbose	–		2.2 ± 0.001		1.3 ± 0.001

EE (%) and TPC (mg GAE · 100 g–1 extract) of the mock privet leaves and fruit.

Extracts	EE		TPC
Extracts	Leaves	Fruit	Leaves	Fruit
Ethyl acetate	2.05 ± 0.003 x	1.47 ± 0.004 x	1,497 ± 1.04 x	1,004 ± 1.19 x
Methanol	16.98 ± 0.010 y	13.83 ± 0.011 y	1,974 ± 3.90 y	1,492 ± 0.23 y
Water	18.02 ± 0.012 y	19.02 ± 0.010 z	2,531 ± 2.75 z	2,260 ± 3.16 z

Analytical characteristics of aqueous extract of mock privet fruit and leaf and their concentration for various solvents used.

Compound name	Morphological part	Ethyl acetate (μg · g^–1 extract)	Methanol (μg · g^–1 extract)	Aqueous (μg · g^–1 extract)	Precursor ion (m/z)	Production (m/z)	Fragmentor energy (V)	Collision energy (V)	Retention time (min)
Flavonols
Rutin	Leaves	430.84 ±3.18	632.16 ±21.78	822.09 ±4.02	609	301	135	35	12.3
	Fruit	24.90 ± 0.10	254.34 ± 4.09	361.94 ± 1.12	609	301	135	35	12.5
Myricetin	Leaves	98 ± 1.63	136.43 ± 2.06	n.d	479	317	135	25	15.30
	Fruit	64 ± 1.08	91 ± 1.38	n.d	479	317	135	25	15.31
Quercetin-3-O-	Leaves	38 ± 1.03	146.43 ± 2.11	34.26 ± 1.03	477	301	135	35	13.2
glucuronide	Fruit	22 ± 1.08	109 ± 0.38	28 ± 0.08	477	301	135	35	13.2
Quercetin	Leaves	29 ± 0.94	204.08 ± 1.06	31.7 ± 0.04	463	301	135	25	12.5
7-O-glucoside	Fruit	26 ± 1.08	121 ± 0.38	30.01 ± 0.08	463	301	135	25	12.5
Luteolin	Leaves	1,098 ± 1.63	2,136.43 ± 12.06	898.23 ± 6.04	447	285	135	25	13.5
7-O-glucoside	Fruit	854 ± 1.08	1,241 ± 1.38	509 ± 0.78	447	285	135	25	13.5
Kaempferol	Leaves	35.12 ± 1.47	148.75 ± 0.32	108.20 ± 2.94	285.2	151	135	20	17.7
aglycone	Fruit	22 ± 0.03	91 ± 0.82	32 ± 0.08	285.2	151	135	20	17.7
Apigenin	Leaves	12 ± 0.07	116.04 ± 2.01	673.91 ± 4.14	269.2	151	135	20	20.6
aglycone	Fruit	n.d	n.d	n.d	269.2	151	135	20	20.6
Anthocyanidins
Cyanidin	Leaves	95 ± 0.50	377.43 ± 6.0	583.17 ± 9.51	595	449	162	30	9.12
3-O-rutinoside	Fruit	101 ± 3.18	641 ± 7.51	1,009 ± 0.78	595	449	162	30	8.99
Cyanidin	Leaves	46.61 ± 0.07	239.40 ± 1.91	398.23 ± 6.04	449	287	162	23	8.07
3-O-glucoside	Fruit	354 ± 1.08	278 ± 0.26	414 ± 11.43	449	287	162	23	8.07
Hydroxycinnamic acids
Ferulic acid	Leaves	233 ± 1.57	316.12 ± 1.45	38.23 ± 1.32	193	149	135	10	13.16
	Fruit	279 ± 1.89	341 ± 1.38	40.92 ± 0.95	193	149	135	10	13.18
Caffeic acid	Leaves	562.17 ± 1.01	1,596 ± 11.37	1,367.40 ± 4.02	179	135	135	15	9.85
	Fruit	202.75 ± 0.70	1,094.04 ± 15.29	615.33 ± 5.42	179	135	135	15	9.85
Rosmarinic acid	Leaves	47 ± 0.54	77.03 ± 1.06	68.23 ± 0.06	359	161	90	20	11.08
	Fruit	29.75 ± 0.68	43.27 ± 0.57	39.87 ± 0.31
5-caffeoy Iquinic	Leaves	498 ± 11.3	636.23 ± 9.84	1,298.23 ± 15.04	353	191	135	30	9.5
acid	Fruit	106 ± 1.08	364 ± 2.31	609 ± 7.91	353	191	135	30	9.4
Secoiridoids
Oleuropein	Leaves	947.93 ± 10.02	571.19 ± 3.16	123.98 ± 2.49	539	275	165	20	6.3
	Fruit	322.05 ± 0.88	149 ± 1.42	87.49 ± 0.91	539	275	165	20	6.3

eISSN:: 2083-5965
Language:: English

Publication timeframe:: 2 times per year
Journal Subjects:: Life Sciences, Plant Science, Zoology, Ecology, other

Journal RSS Feed

Phytochemicals from Phillyrea latifolia L. leaves and fruit extracted with various solvents: Their identification and quantification by LC-MS and antihyperglycemic effects

Article Category: ORIGINAL ARTICLE

Published Online: Jun 26, 2023

Page range: 233 - 242

Received: Feb 15, 2023

Accepted: Jun 12, 2023

DOI: https://doi.org/10.2478/fhort-2023-0018

Keywordsantidiabetic, antioxidant, LC-MS, mock privet, phytochemicals

© 2023 Ebru Aydin, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Antioxidant, α-amylase and α-glucosidase activities (IC50 mg · mL–1) for mock privet leaf and fruit extracts and acarbose 1 mg · mL–1.

EE (%) and TPC (mg GAE · 100 g–1 extract) of the mock privet leaves and fruit.

Analytical characteristics of aqueous extract of mock privet fruit and leaf and their concentration for various solvents used.

Keywords
antidiabetic, antioxidant, LC-MS, mock privet, phytochemicals