<abstract xmlns="http://www.w3.org/1999/xhtml"><p>We previously demonstrated that remote infrared Matrix Assisted Laser Desorption Ionization technology (Spidermass) using endogenous water as matrix (or so called water assisted laser desorption/ionization) was enabling real-time <italic>in vitro</italic> and <italic>in vivo</italic> analysis of clinical pathological tissues. In the present work, Spidermass was used to biotype human pathogens either from liquid bacteria growth in time course, from petri dish or on smears. Reproducibility experiments as well as bacteria dispersion and lipids identifications with SpiderMass in MS/MS mode were undertaken. The whole of the data establish that SpiderMass instrument allows real time bacteria biotyping and can be useful in clinic for pathogen identification.</p></abstract>

We previously demonstrated that remote infrared Matrix Assisted Laser Desorption Ionization technology (Spidermass) using endogenous water as matrix (or so called water assisted laser desorption/ionization) was enabling real-time in vitro and in vivo analysis of clinical pathological tissues. In the present work, Spidermass was used to biotype human pathogens either from liquid bacteria growth in time course, from petri dish or on smears. Reproducibility experiments as well as bacteria dispersion and lipids identifications with SpiderMass in MS/MS mode were undertaken. The whole of the data establish that SpiderMass instrument allows real time bacteria biotyping and can be useful in clinic for pathogen identification.

Real time human micro-organisms biotyping based on Water-Assisted Laser Desorption/Ionization

Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM)

The EuroBiotech Journal

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

We previously demonstrated that remote infrared Matrix Assisted Laser Desorption Ionization technology (Spidermass) using endogenous water as matrix (or so...

Real time human micro-organisms biotyping based on Water-Assisted Laser Desorption/Ionization

Article Category: Research Article

Published Online: Apr 24, 2019

Page range: 97 - 104

DOI: https://doi.org/10.2478/ebtj-2019-0011

Keywords
Spidermass, real time analysis, mass spectrometry, bacteria typing

© 2019 Benoit Fatou, Michel Salzet, Isabelle Fournier, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Supplementary Material Details

Real time human micro-organisms biotyping based on Water-Assisted Laser Desorption/Ionization

Article Category: Research Article

Published Online: Apr 24, 2019

Page range: 97 - 104

DOI: https://doi.org/10.2478/ebtj-2019-0011

KeywordsSpidermass, real time analysis, mass spectrometry, bacteria typing

© 2019 Benoit Fatou, Michel Salzet, Isabelle Fournier, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Supplementary Material Details

Keywords
Spidermass, real time analysis, mass spectrometry, bacteria typing