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Topical Helichrysum italicum Essential Oil-Based Ointment Hastens Excisional Wound Healing by Alleviating Inflammation and Oxidative Stress in Diabetic Rats

Marijana Andjic

Marijana Andjic

  • University of Kragujevac, Faculty of Medical Sciences, Department of PharmacyKragujevac, Serbia
  • Center of Excellence for Redox Balance Research in Cardiovascular and Metabolic DisordersKragujevac, Serbia
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Andjic, Marijana
, 
Aleksandar Kocovic

Aleksandar Kocovic

  • University of Kragujevac, Faculty of Medical Sciences, Department of PharmacyKragujevac, Serbia
  • Center of Excellence for Redox Balance Research in Cardiovascular and Metabolic DisordersKragujevac, Serbia
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Kocovic, Aleksandar
, 
Ksenija Vucicevic

Ksenija Vucicevic

University of Kragujevac, Faculty of Medical Sciences, Department of PharmacyKragujevac, Serbia
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Vucicevic, Ksenija
, 
Vladimir Jakovljevic

Vladimir Jakovljevic

  • Center of Excellence for Redox Balance Research in Cardiovascular and Metabolic DisordersKragujevac, Serbia
  • Department of Human Pathology, 1st Moscow State Medical, University IM SechenovMoscow, Russia
  • University of Kragujevac, Faculty of Medical Sciences, Department of PhysiologyKragujevac, Serbia
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Jakovljevic, Vladimir
, 
Igor Simanic

Igor Simanic

  • Specialized Hospital for Rehabilitation and Orthopedic ProstheticsBeograd, Serbia
  • University of Kragujevac, Faculty of Medical Sciences, Department of Physical Medicine and RehabilitationKragujevac, Serbia
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Simanic, Igor
, 
Milica Vukasinovic Vesic

Milica Vukasinovic Vesic

Faculty of Sport, University “Union-Nikola Tesla”Belgrade, Serbia
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Vukasinovic Vesic, Milica
 and 
Jovana Bradic

Jovana Bradic

  • University of Kragujevac, Faculty of Medical Sciences, Department of PharmacyKragujevac, Serbia
  • Center of Excellence for Redox Balance Research in Cardiovascular and Metabolic DisordersKragujevac, Serbia
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Bradic, Jovana
  
Dec 12, 2024
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Article Category: Original Scientific Article
Published Online: Dec 12, 2024
Received: Sep 09, 2024
Accepted: Sep 23, 2024
DOI: https://doi.org/10.2478/eabr-2024-0012
Keywords
© 2024 Marijana Andjic et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1.

Photograph of H. italicum essential oil – based ointment
Photograph of H. italicum essential oil – based ointment

Figure 2.

Effects of applied ointments on the wound contraction.Values are expressed as mean ± standard deviation (n = 8). a p < 0.05 compared to CTRL group; b p < 0.05 compared to SSD group; c p < 0.05 compared to OINT group; d p < 0.05 compared to HIOINT group. CTRL – control; SSD – silver sulfadiazine; OINT – ointment base; HIOINT – H. italicum ointment;
Effects of applied ointments on the wound contraction.Values are expressed as mean ± standard deviation (n = 8). a p < 0.05 compared to CTRL group; b p < 0.05 compared to SSD group; c p < 0.05 compared to OINT group; d p < 0.05 compared to HIOINT group. CTRL – control; SSD – silver sulfadiazine; OINT – ointment base; HIOINT – H. italicum ointment;

Figure 3.

Effects of applied ointments on the markers inflammation: A) TNF-α; B) IL-6; C) IL-10. Values are expressed as mean ± standard deviation (n = 8).a p < 0.05 compared to CTRL group; b p < 0.05 compared to SSD group; c p < 0.05 compared to OINT group; d p < 0.05 compared to HIOINT group. CTRL – control; SSD – silver sulfadiazine; OINT – ointment base; HIOINT – H. italicum ointment;
Effects of applied ointments on the markers inflammation: A) TNF-α; B) IL-6; C) IL-10. Values are expressed as mean ± standard deviation (n = 8).a p < 0.05 compared to CTRL group; b p < 0.05 compared to SSD group; c p < 0.05 compared to OINT group; d p < 0.05 compared to HIOINT group. CTRL – control; SSD – silver sulfadiazine; OINT – ointment base; HIOINT – H. italicum ointment;

Figure 4.

Effects of applied ointments on the oxidative stress markers in wound tissue: A) TBARS; B) SOD; C) CAT; D) GSH. Values are expressed as mean ± standard deviation (n = 8).a p < 0.05 compared to CTRL group; b p < 0.05 compared to SSD group; c p < 0.05 compared to OINT group; d p < 0.05 compared to HIOINT group. CTRL – control; SSD – silver sulfadiazine; OINT – ointment base; HIOINT – H. italicum ointment;
Effects of applied ointments on the oxidative stress markers in wound tissue: A) TBARS; B) SOD; C) CAT; D) GSH. Values are expressed as mean ± standard deviation (n = 8).a p < 0.05 compared to CTRL group; b p < 0.05 compared to SSD group; c p < 0.05 compared to OINT group; d p < 0.05 compared to HIOINT group. CTRL – control; SSD – silver sulfadiazine; OINT – ointment base; HIOINT – H. italicum ointment;

Organoleptic characteristics and spreadability of ointment with H_ italicum essential oil after 3 and 180 days of preparation

Parameters 3 days 180 days
Colour Yellow Yellow
Odour Characteristic odor of the essential oil Characteristic odor of the essential oil
Consistency Semi-solid Semi-solid
Homogenity Homogeneous ointment Homogeneous ointment
Spreadability Easily Easily

Ointment base and H_ italicum essential oil –based ointment formulations_

Ingredient Ointment Base (%) Ointment HIEO (%)
HIEO / 0.50
Cholesterol 5.00 5.00
Lanolin 15.00 15.00
Parafinum liquidum 15.00 15.00
Vaselinum album 65.00 ad 65.00
Language:
English
Publication timeframe:
4 times per year
Journal Subjects:
Medicine, Clinical Medicine, Clinical Medicine, other
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