Here, we present the latest findings on moth fly (Psychodidae: Diptera) species richness in the Moravskoslezské Beskydy Mts. and Podbeskydská Pahorkatina Uplands, based on both previously published and unpublished data and new samples collected between 2007 and 2020. Two species new to the fauna of the Czech Republic were recorded (Sycorax feuerborni Jung, 1954 and Jungiella (Jungiella) revelica Vaillant, 1972), bringing the total number of moth fly species in the Czech Republic to 180 (Ježek et al. 2021). In addition, five species were recorded for the first time in the area of interest: Trichomyia parvula Szabó, 1960 (second locality in the Czech Republic); Paramormia (Phyllotelmatoscopus) acuta (Krek, 1971) (third record in the Czech Republic); Philosepedon (Philosepedon) perdecorum Omelková & Ježek, 2012 (third locality and fourth record); Pericoma (Pericoma) ljubiniensis Krek, 1969 (second finding and third locality); Tonnoiriella pulchra (Eaton, 1893) (sixth area and first finding in the Czech Carpathians). The finding of Jungiella (Jungiella) valachica (Vaillant, 1963) confirms its historical occurrence in the region. In the latest survey, we collected 108 species from 29 localities, of which 16 are critically endangered, eight endangered and three vulnerable. Nine localities were evaluated as species-rich, i.e. Hluchý 1. (58 species), Vysutý 2. (56), Hluchý 2. (53), Vysutý 4. (49), Skalická Morávka 2. (46), Chladná voda 3. (44), Hluchý 3. (43), Morávka - spring and Obidová 1. (both 42). The total number of moth flies found within the Moravskoslezské Beskydy Mts. and Podbeskydská pahorkatina Uplands now stands at 124 species, representing 68.9 % of total Czech fauna.
