Publications up to 2011 record 21 species of stonefly, infraorder Systellognatha, as occurring in the Moravskoslezské Beskydy Mts. and Podbeskydská pahorkatina Uplands; however, seven of these cannot be considered part of the fauna of this area as they probably never occurred there, and one species (Perla abdominalis Burmeister, 1839) was not confirmed, despite being demonstrably recorded in 2000. In the present study, the current stonefly fauna was confirmed at 14 species, belonging to three families and eight genera. The occurrence of Isoperla sudetica (Kolenati, 1859) is published for the first time. Among the most important findings are confirmation of the occurrence of two endangered species (Perla grandis (Rambur, 1841) and Arcynopteryx dichroa (McLachlan, 1872)) and one near threatened species (Isoperla buresi Raušer, 1962). Equally important is confirmation of the occurrence of Perlodes intricatus (Pictet, 1841), based on egg morphology and DNA barcoding.