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The expression levels of microRNAs associated with T and B cell differentiation/stimulation in ankylosing spondylitis

A Türkyilmaz
A Türkyilmaz
, 
P Ata
P Ata
, 
F Akbaş
F Akbaş
 and 
İ Yağci
İ Yağci
   | Aug 26, 2020
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Article Category: Original Article
Published Online: Aug 26, 2020
Page range: 25 - 32
DOI: https://doi.org/10.2478/bjmg-2020-0006
Keywords
© 2020 Türkyilmaz A, Ata P, Akbaş F, Yağci İ, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Comparison of expression levels of miRNA in the patient and control groups.

miRNAAVG Ct2[-AvgΔ(Ct)]p ValueRatio Patient/Control
ControlPatientControlPatient
miR-14326.0025.040.1361390.2038920.0257211.4977
miR-142-5p26.8024.680.0784350.262170.0000033.3425

Distribution of disease characteristics.

Patient Group (n=50)n(%)
HLA-B27negative

positive

23 (46.0)

27 (54.0)

ESRmedian (min-max)

mean+SD

17 (2-59)

21.36±15.51

Symptom duration (years)median (min-max)

mean+SD

5 (1-25)

6.60±5.08

BASDAImedian (min-max)

mean±SD

2.5 (0-8)

2.75±2.03

BASFmedian (min-max)

mean+SD

5(5-12)

5.36±1.19

BASMImedian (min-max)

mean+SD

1 (0-9.8)

1.55±1.93

miR-142-5pmedian (min-max)

mean±SD

24.1 (21.4-30.5)

24.68±2.06

miR-143median (min-max)

mean+SD

24.2 (21.6-35.8)

25.10±3.11

Control Group (n=50)n (%)
miR-142-5pmedian (min-max)

mean±SD

26.4 (22.45-34.7)

26.88±2.88

miR-143median (min-max)

mean+SD

25.4(21.28-33.7)

26.02±2.71

Correlation between age and disease characteristics and miR-142-5p and miR-143 measurements.

Patient Group (n=50)miR-142-5pmiR-143
r

Pearson correlation coefficient.

p Valuer

Spearman’s correlation coefficient.

p Value
Age (years)0.0470.7460.1260.384
ESR-0.0700.641-0.0810.589
Symptom duration (years)-0.1840.200-0.1720.233
BASDAI-0.2680.060-0.2660.062
BASFI-0.0260.861-0.1140.447
BASMI0.0620.6710.2290.110

Distribution of descriptive characteristics.

Descriptive Characteristics (n=100)
Age (years)median (min-max)37(18-60)
mean±SD37.24±9.42
Gender n (%)females58 (58.0)
males42 (42.0)

miRNAs analyzed in the signaling pathway targeted miScript miRNA PCR array study.

T-Cell Differentiation

CD4-/CD8- T-cell associated:

let-7d-5p, let-7e-5p, miR-126-3p, miR-128, miR-146b-5p, miR-15a-5p, miR-17-5p,

miR-17-3p, miR-181c-5p, miR-191-5p, miR-19a-3p, miR-199a-5p, miR-20a-5p, miR-20b-5p,

miR-221-3p, miR-222-3p, miR-223-3p, miR-28-5p, miR-29c-3p, miR-30e-5p, miR-342-3p,

miR-423-5p, miR-93-5p, miR-98-5p

CD4+/CD8+ T-cell associated:let-7b-5p, miR-181a-5p, miR-181b-5p, miR-181d, miR-19b-3p.
CD4+ Naive T-cell associated:miR-132-3p, miR-146a-5p, miR-182-5p, miR-184, miR-25-3p, miR-326, miR-92a-3p
CD8+ Naive T-cell associated:let-7a-5p, let-7c, let-7f-5p, let-7g-5p, miR-130b-3p, miR-139-5p, miR-142-3p,

miR-150-5p, miR-155-5p, miR-15a-3p, miR-16-5p, miR-26a-5p, miR-26b-5p,

miR-29b-3p, miR-30b-5p, miR-30c-5p, miR-30d-5p

CD8+ Effector cell associated:miR-147a, miR-148a-3p, miR-18a-5p, miR-27a-3p, miR-27b-3p
CD8+ Memory cell associated:let-7i-5p, miR-106b-5p, miR-142-5p, miR-15b-5p, miR-17-5p, miR-21-5p, miR-23a-3p,

miR-23b-3p, miR-24-3p, miR-29a-3p, miR-31-5p

B-Cell Differentiationlet-7a-5p, let-7b-5p, let-7d-5p, let-7g-5p, let-7i-5p, miR-101-3p, miR-132-3p, miR-142-3p,
Naive B-cell associated:miR-142-5p, miR-150-5p, miR-181c-5p, miR-195-5p, miR-204-5p, miR-214-3p, miR-221-3p,

miR-222-3p, miR-223-3p, miR-29b-3p, miR-30e-5p, miR-331-3p, miR-92a-3p

Germinal centre associated:miR-106b-5p, miR-130b-3p, miR-132-3p, miR-148a-3p, miR-15a-5p, miR-15b-5p, miR-16-5p,

miR-17-5p, miR-17-3p, miR-181a-5p, miR-181b-5p, miR-191-5p, miR-19a-3p, miR-19b-3p,

miR-210, miR-23b-3p, miR-25-3p, miR-28-5p, miR-30d-5p, miR-93-5p, miR-98-5p

Memory cell associated:miR-100-5p, miR-125b-5p, miR-145-5p, miR-146a-5p, miR-155-5p, miR-21-5p, miR-23a-3p,

miR-24-3p, miR-26a-5p, miR-26b-5p, miR-27a-3p, miR-27b-3p, miR-29a-3p, miR-29c-3p,

miR-30b-5p, miR-30c-5p, miR-34a-5p

Expressed in the Treg cells:miR-100-5p, miR-125b-5p, miR-146a-5p, miR-181c-5p, miR-20b-5p, miR-21-5p, miR-31-5p,

miR-335-5p, miR-365a-3p, miR-99a-5p

T-cell activation associated:let-7e-5p, let-7g-5p, let-7i-5p, miR-143, miR-140-3p, miR-142-5p, miR-146a-5p,

miR-146b-5p, miR-155-5p, miR-15a-5p, miR-15a-3p, miR-181a-5p, miR-181c-5p,

miR-195-5p, miR-20b-5p, miR-214-3p, miR-223-3p, miR-23a-3p, miR-23b-3p, miR-25-3p,

miR-26a-5p, miR-26b-5p, miR-27a-3p, miR-27b-3p, miR-29a-3p, miR-29b-3p, miR-29c-3p,

miR-30a-5p, miR-30b-5p, miR-30e-5p, miR-342-3p, miR-368, miR-574-3p, miR-98-5p

eISSN:
1311-0160
Language:
English
Publication timeframe:
2 times per year
Journal Subjects:
Medicine, Basic Medical Science, other
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