<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>A nutritional study was conducted to evaluate the influence of dietary fish oil (FO) sparing by using a mixture of vegetal oils (VO) on rainbow trout (<italic>Oncorhynchus mykiss</italic>) juveniles (30.0 g). A basal diet was supplemented with the experimental oils at a 10% level, including FO (FO diet), a blend of VO (sunflower, soybean and rapeseed oils with a 1:1:1 ratio), and a mixture (1:1 ratio) of FO and the blends of VO (MIX). In a raceway system, 450 fish were stocked in nine rectangular concrete tanks (50 fish/ tank<sup>−1</sup>). Three experimental diets, with three replicates each, were offered to fish up to visual satiation for eight weeks. Growth performance did not change among groups (P>0.05). The highest and lowest proportions of n-3 polyunsaturated fatty acids (PUFA), n-3 long chain-PUFA, especially docosahexaenoic acid, and n-3/n-6 PUFA ratio were in the whole body of FO and the VO groups, respectively (P&lt;0.05). Serum catalase and glutathione peroxidase activities in VO group were higher than others. Serum superoxide dismutase activity in fish fed FO and VO diets was higher than MIX group (P&lt;0.05). VO group had higher serum lysozyme, alternative complement pathway, and total immunoglobulin levels than other experimental groups. MIX group had highest serum triglyceride, cholesterol, and low-density lipoproteins. Serum high-density lipoproteins, aspartate aminotransferase, and alanine aminotransferase levels in VO treatment were in the lowest level compared to others (P&lt;0.05). The highest relative transcription levels of fatty acid synthase, delta-6 fatty acyl desaturase, and apolipoprotein b-100 genes were in the liver of fish fed the MIX diet. The relative transcription level of the fatty acid-binding protein1 gene did not change (P>0.05). In summary, the results of this study revealed that dietary FO sparing with VO did not adversely affect the growth and health indices of rainbow trout; nevertheless, it had adverse effects on its nutritional values by reducing whole-body n-3 LC-PUFA content.</p>
</abstract>

A nutritional study was conducted to evaluate the influence of dietary fish oil (FO) sparing by using a mixture of vegetal oils (VO) on rainbow trout (Oncorhynchus mykiss) juveniles (30.0 g). A basal diet was supplemented with the experimental oils at a 10% level, including FO (FO diet), a blend of VO (sunflower, soybean and rapeseed oils with a 1:1:1 ratio), and a mixture (1:1 ratio) of FO and the blends of VO (MIX). In a raceway system, 450 fish were stocked in nine rectangular concrete tanks (50 fish/ tank−1). Three experimental diets, with three replicates each, were offered to fish up to visual satiation for eight weeks. Growth performance did not change among groups (P>0.05). The highest and lowest proportions of n-3 polyunsaturated fatty acids (PUFA), n-3 long chain-PUFA, especially docosahexaenoic acid, and n-3/n-6 PUFA ratio were in the whole body of FO and the VO groups, respectively (P<0.05). Serum catalase and glutathione peroxidase activities in VO group were higher than others. Serum superoxide dismutase activity in fish fed FO and VO diets was higher than MIX group (P<0.05). VO group had higher serum lysozyme, alternative complement pathway, and total immunoglobulin levels than other experimental groups. MIX group had highest serum triglyceride, cholesterol, and low-density lipoproteins. Serum high-density lipoproteins, aspartate aminotransferase, and alanine aminotransferase levels in VO treatment were in the lowest level compared to others (P<0.05). The highest relative transcription levels of fatty acid synthase, delta-6 fatty acyl desaturase, and apolipoprotein b-100 genes were in the liver of fish fed the MIX diet. The relative transcription level of the fatty acid-binding protein1 gene did not change (P>0.05). In summary, the results of this study revealed that dietary FO sparing with VO did not adversely affect the growth and health indices of rainbow trout; nevertheless, it had adverse effects on its nutritional values by reducing whole-body n-3 LC-PUFA content.

Effect of dietary fish oil sparing with blended vegetable oils on growth, fatty acid composition and lipid-metabolism-related genes expression in juvenile rainbow trout (<italic>Oncorhynchus mykiss</italic>)

Annals of Animal Science

This work is licensed under the Creative Commons Attribution 4.0 International License.

Effect of dietary fish oil sparing with blended vegetable oils on growth, fatty acid composition and lipid-metabolism-related genes expression in juvenile rainbow trout ()

A nutritional study was conducted to evaluate the influence of dietary fish oil (FO) sparing by using a mixture of vegetal oils (VO) on rainbow trout (Oncorhynchus mykiss) juveniles (30.0 g). A basal diet was supplemented with the experimental oils at a 10% level, including FO (FO diet), a blend of VO (sunflower, soybean and rapeseed oils with a 1:1:1 ratio), and a mixture (1:1 ratio) of FO and the blends of VO (MIX). In a raceway system, 450 fish were stocked in nine rectangular concrete tanks (50 fish/ tank−1). Three experimental diets, with three replicates each, were offered to fish up to visual satiation for eight weeks. Growth performance did not change among groups (P>0.05). The highest and lowest proportions of n-3 polyunsaturated fatty acids (PUFA), n-3 long chain-PUFA, especially docosahexaenoic acid, and n-3/n-6 PUFA ratio were in the whole body of FO and the VO groups, respectively (P0.05). In summary, the results of this study revealed that dietary FO sparing with VO did not adversely affect the growth and health indices of rainbow trout; nevertheless, it had adverse effects on its nutritional values by reducing whole-body n-3 LC-PUFA content.

A nutritional study was conducted to evaluate the influence of dietary fish oil (FO) sparing by using a mixture of vegetal oils (VO) on rainbow trout...

Effect of dietary fish oil sparing with blended vegetable oils on growth, fatty acid composition and lipid-metabolism-related genes expression in juvenile rainbow trout (Oncorhynchus mykiss)

Published Online: Apr 23, 2024

Page range: 519 - 531

Received: May 06, 2023

Accepted: Jul 26, 2023

DOI: https://doi.org/10.2478/aoas-2023-0083

Keywords
Δ-6-fatty acid desaturase, liver enzymes, salmonids, super-oxidase dismutase, long-chain polyunsaturated fatty acid

© 2024 Alireza Ghaedi et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Effect of dietary fish oil sparing with blended vegetable oils on growth, fatty acid composition and lipid-metabolism-related genes expression in juvenile rainbow trout (Oncorhynchus mykiss)

Published Online: Apr 23, 2024

Page range: 519 - 531

Received: May 06, 2023

Accepted: Jul 26, 2023

DOI: https://doi.org/10.2478/aoas-2023-0083

KeywordsΔ-6-fatty acid desaturase, liver enzymes, salmonids, super-oxidase dismutase, long-chain polyunsaturated fatty acid

© 2024 Alireza Ghaedi et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
Δ-6-fatty acid desaturase, liver enzymes, salmonids, super-oxidase dismutase, long-chain polyunsaturated fatty acid