<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Sarcoids are the most common dermatological tumor of equids worldwide. These fibroblastic tumors rarely regress, more often they remain and can be locally invasive. To explore the functional importance of DNA methylation during the BPV-dependent neoplasia of equine dermal tissues, this study assessed a methylation level of CpG islands (CGIs) in the promoter region of <italic>LPAR1</italic>, <italic>MPZL3</italic>, and <italic>POPDC2</italic> genes, and within the gene body of the <italic>EHF</italic> gene, which were found to be differentially expressed in sarcoid samples in a previous study and harbored the differentially methylated sites (DMSs) in their sequences. The results of bisulfite sequencing PCR (BSP) showed a decrease in the methylation levels of CGIs of <italic>LPAR1</italic> and <italic>EHF</italic> genes and an increase in the case of <italic>POPDC2</italic>. The obtained data did not reveal any changes in the level of DNA methylation in the analyzed promoter region of the <italic>MPZL3</italic> gene between the tumor and healthy tissues. Our observations confirm the presence of CpG methylation alterations in the equine sarcoid and provide new insight into a better understanding of the epigenetic regulation of this tumor development.</p>
</abstract>

Sarcoids are the most common dermatological tumor of equids worldwide. These fibroblastic tumors rarely regress, more often they remain and can be locally invasive. To explore the functional importance of DNA methylation during the BPV-dependent neoplasia of equine dermal tissues, this study assessed a methylation level of CpG islands (CGIs) in the promoter region of LPAR1, MPZL3, and POPDC2 genes, and within the gene body of the EHF gene, which were found to be differentially expressed in sarcoid samples in a previous study and harbored the differentially methylated sites (DMSs) in their sequences. The results of bisulfite sequencing PCR (BSP) showed a decrease in the methylation levels of CGIs of LPAR1 and EHF genes and an increase in the case of POPDC2. The obtained data did not reveal any changes in the level of DNA methylation in the analyzed promoter region of the MPZL3 gene between the tumor and healthy tissues. Our observations confirm the presence of CpG methylation alterations in the equine sarcoid and provide new insight into a better understanding of the epigenetic regulation of this tumor development.

Involvement of Aberrant DNA Methylation in the Deregulated Expression of <italic>EHF, LPAR1</italic>, <italic>MPZL3,</italic> and <italic>POPDC2</italic> Genes in Equine Sarcoids

Department of Animal Anatomy and Preclinical Sciences, University Centre of Veterinary Medicine JU-UA

University Centre of Veterinary Medicine JU-UA, University of Agriculture in Krakow

Annals of Animal Science

This work is licensed under the Creative Commons Attribution 4.0 International License.

Involvement of Aberrant DNA Methylation in the Deregulated Expression of ,  and  Genes in Equine Sarcoids

Sarcoids are the most common dermatological tumor of equids worldwide. These fibroblastic tumors rarely regress, more often they remain and can be locally...

Involvement of Aberrant DNA Methylation in the Deregulated Expression of EHF, LPAR1, MPZL3, and POPDC2 Genes in Equine Sarcoids

Published Online: Jan 23, 2024

Page range: 99 - 107

Received: Apr 03, 2023

Accepted: Jul 06, 2023

DOI: https://doi.org/10.2478/aoas-2023-0078

Keywords
equine sarcoid, DMSs, DEGs, DNA methylation, gene expression, tumor

© 2024 Ewelina Semik-Gurgul et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Involvement of Aberrant DNA Methylation in the Deregulated Expression of EHF, LPAR1, MPZL3, and POPDC2 Genes in Equine Sarcoids

Published Online: Jan 23, 2024

Page range: 99 - 107

Received: Apr 03, 2023

Accepted: Jul 06, 2023

DOI: https://doi.org/10.2478/aoas-2023-0078

Keywordsequine sarcoid, DMSs, DEGs, DNA methylation, gene expression, tumor

© 2024 Ewelina Semik-Gurgul et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
equine sarcoid, DMSs, DEGs, DNA methylation, gene expression, tumor