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CRISPR/Cas System: An Effective Tool Against Pathogenic Diseases

Shakira Aslam

Shakira Aslam

Center for Applied Molecular Biology, University of the PunjabLahore, Pakistan
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Aslam, Shakira
, 
Ali Umair

Ali Umair

Mohi-ud-Din Islamic Medical CollegeMirpur, Pakistan
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Umair, Ali
, 
Zaid Aslam

Zaid Aslam

Mohi-ud-Din Islamic Medical CollegeMirpur, Pakistan
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Aslam, Zaid
, 
Muhammad Zafar Saleem

Muhammad Zafar Saleem

Center for Applied Molecular Biology, University of the PunjabLahore, Pakistan
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Saleem, Muhammad Zafar
 and 
Hamid Bashir

Hamid Bashir

Center for Applied Molecular Biology, University of the PunjabLahore, Pakistan
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Bashir, Hamid
  
Jun 26, 2023
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Published Online: Jun 26, 2023
Page range: 87 - 99
Received: Nov 01, 2022
Accepted: Dec 01, 2022
DOI: https://doi.org/10.2478/am-2023-0009
Keywords
© 2023 Shakira Aslam et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

CRISPR/Cas system mechanism of action
CRISPR/Cas system mechanism of action

Fig. 2.

Anti-SARS-CoV-2 effect produced by CRISPR/Cas13d that inhibits the replication of virus by crRNA-guided viral mRNA and genome degradation
Anti-SARS-CoV-2 effect produced by CRISPR/Cas13d that inhibits the replication of virus by crRNA-guided viral mRNA and genome degradation

Fig. 3.

Mechanism of ABACAS strategy. ABACAS strategy captures S protein of Coronavirus and successfully delivers the components of CRISPR into infected cells. CRISPR components then attack viral RNA and degrade it. This strategy also helps in preventing viral entry into cell
Mechanism of ABACAS strategy. ABACAS strategy captures S protein of Coronavirus and successfully delivers the components of CRISPR into infected cells. CRISPR components then attack viral RNA and degrade it. This strategy also helps in preventing viral entry into cell

Fig. 4.

CRISPR-Cas strategy against herpes virus [108]
CRISPR-Cas strategy against herpes virus [108]

Fig. 5.

Limitations of CRISPR/Cas
Limitations of CRISPR/Cas

CRISPR/Cas systems for elimination and targeting HIV-1 provirus

CRISSPR-Cas system used Delivery method for CRISPR components Targeted regions Cell line or organism studied Targeted locus Efficiency References
SpCas9 Transfection LTR (U3 region) 293T, Jurkat & Hela 465–484 30–90% [104]
SpCas9 Transfection LTR (U3 region) CHME5, TZM-BI, & U937 101–127312–341 30–90% [105]
SpCas9 Lentivirus LTR (R region) 293T-CD4-CCR5, hPSC, & 293 Primary T cells 464–486485–507 20–90% [107]
SpCas9 Transfection Rev (the second exon) JLat10.6 8513–8532 30% [93]
SpCas9 Lentivirus Gag/ Pol/ Rev/ Env SupT1 2249–22778497–8525 30–90% [135]
SpCas9 Lentivirus LTR (U3 and R region) J.Lat FL & SupT1 300–408463–482 35–98% [108]
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