<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Viral pathogens are major concern nowadays. Bacterial CRISPR/Cas systems help in defending the host body against different pathogens including viruses too. This system contains restriction enzymes that introduce dsDNA breaks on target site to make the virus non-functional by damaging its genes. Coronavirus, HIV and Herpes viruses are causing mortality all around the world. To control the spread of disease, early detection and treatment is required. CRISPR system due to its high efficiency provides a platform to restrict the viral entry into host cell, viral genome editing and eliminate the latent infections. In this review, the CRISPR strategies against three major viral diseases are put under consideration: Coronavirus, Acquired Immunodeficiency Syndrome and Herpesvirus associated diseases. Three CRISPR/Cas systems have been discussed including CRISPR/Cas12, CRISPR/Cas9 and CRISPR/Cas13 that are used against the above listed viruses.</p>
</abstract>

Viral pathogens are major concern nowadays. Bacterial CRISPR/Cas systems help in defending the host body against different pathogens including viruses too. This system contains restriction enzymes that introduce dsDNA breaks on target site to make the virus non-functional by damaging its genes. Coronavirus, HIV and Herpes viruses are causing mortality all around the world. To control the spread of disease, early detection and treatment is required. CRISPR system due to its high efficiency provides a platform to restrict the viral entry into host cell, viral genome editing and eliminate the latent infections. In this review, the CRISPR strategies against three major viral diseases are put under consideration: Coronavirus, Acquired Immunodeficiency Syndrome and Herpesvirus associated diseases. Three CRISPR/Cas systems have been discussed including CRISPR/Cas12, CRISPR/Cas9 and CRISPR/Cas13 that are used against the above listed viruses.

CRISPR/Cas System: An Effective Tool Against Pathogenic Diseases

Advancements of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

{"article-title":"CRISPR/Cas System: An Effective Tool Against Pathogenic Diseases"}

Viral pathogens are major concern nowadays. Bacterial CRISPR/Cas systems help in defending the host body against different pathogens including viruses too....

CRISSPR-Cas system used	Delivery method for CRISPR components	Targeted regions	Cell line or organism studied	Targeted locus	Efficiency	References
SpCas9	Transfection	LTR (U3 region)	293T, Jurkat & Hela	465–484	30–90%	[104]
SpCas9	Transfection	LTR (U3 region)	CHME5, TZM-BI, & U937	101–127312–341	30–90%	[105]
SpCas9	Lentivirus	LTR (R region)	293T-CD4-CCR5, hPSC, & 293 Primary T cells	464–486485–507	20–90%	[107]
SpCas9	Transfection	Rev (the second exon)	JLat10.6	8513–8532	30%	[93]
SpCas9	Lentivirus	Gag/ Pol/ Rev/ Env	SupT1	2249–22778497–8525	30–90%	[135]
SpCas9	Lentivirus	LTR (U3 and R region)	J.Lat FL & SupT1	300–408463–482	35–98%	[108]

CRISPR/Cas System: An Effective Tool Against Pathogenic Diseases

Pubblicato online: 26 giu 2023

Pagine: 87 - 99

Ricevuto: 01 nov 2022

Accettato: 01 dic 2022

DOI: https://doi.org/10.2478/am-2023-0009

Parole chiave
AIDS, ABACAS, COVID-19, CRISPR/Cas system, DETECTR, Herpesvirus, PAC-MAN, SHERLOCK

© 2023 Shakira Aslam et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Fig. 2.

Fig. 3.

Fig. 4.

Fig. 5.

CRISPR/Cas systems for elimination and targeting HIV-1 provirus

CRISPR/Cas System: An Effective Tool Against Pathogenic Diseases

Pubblicato online: 26 giu 2023

Pagine: 87 - 99

Ricevuto: 01 nov 2022

Accettato: 01 dic 2022

DOI: https://doi.org/10.2478/am-2023-0009

Parole chiaveAIDS, ABACAS, COVID-19, CRISPR/Cas system, DETECTR, Herpesvirus, PAC-MAN, SHERLOCK

© 2023 Shakira Aslam et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Fig. 2.

Fig. 3.

Fig. 4.

Fig. 5.

CRISPR/Cas systems for elimination and targeting HIV-1 provirus

Parole chiave
AIDS, ABACAS, COVID-19, CRISPR/Cas system, DETECTR, Herpesvirus, PAC-MAN, SHERLOCK