Open Access

Efficacy of medicinal plants from Algeria on cell proliferation: Involvement of caspases 3/7 and cell cycle disorder

Leila Gadouche

Leila Gadouche

  • Laboratory of Natural Bio-Resources, Department of Biology, Faculty of Natural Sciences and Life, Hassiba Benbouali University of ChlefChlef, Algeria
  • TAMAYOUZ Laboratory Endocrinology and Metabolism (LEM)Algier, Algeria
Orcid profile
Search for this author on
Sciendo|Google Scholar
Gadouche, Leila
, 
Dagmara Klopotowska

Dagmara Klopotowska

Department of Experimental Oncology, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of SciencesWroclaw, Poland
Orcid profile
Search for this author on
Sciendo|Google Scholar
Klopotowska, Dagmara
 and 
Joanna Wietrzyk

Joanna Wietrzyk

Department of Experimental Oncology, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of SciencesWroclaw, Poland
Orcid profile
Search for this author on
Sciendo|Google Scholar
Wietrzyk, Joanna
  
Mar 19, 2025
Efficacy of medicinal plants from Algeria on cell proliferation: Involvement of caspases 3/7 and cell cycle disorder's Cover Image
About this article
Previous Article
Next Article
Cite
Download Cover
Article Category: Original Study
Published Online: Mar 19, 2025
Page range: 16 - 24
Received: Sep 18, 2024
Accepted: Jan 15, 2025
DOI: https://doi.org/10.2478/ahem-2025-0003
Keywords
© 2025 Leila Gadouche et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Introduction

Cancer is the main cause of mortality worldwide, and finding effective treatments with fewer side effects is crucial. Medicinal plants offer significant potential in this area. They contain bioactive compounds that may have antitumor effects. The aim of this study is to assess the cytotoxicity of various medicinal plants towards several cancer cell lines.

Materials and Methods

Antiproliferative activity of extracts from Crataegus monogyna, Rhamnus alaternus, Lavandula dentata, Aristolochia baetica, Erica arborea, Cistus lanifedus on cell lines: A-498 (kidney carcinoma), HepG2 (hepatocellular carcinoma), PLC/PRF/5 (hepatoma), MDA-MB-231 (breast adenocarcinoma), MV-4-11 (biphenotypic B myelomonocytic leukemia), and one non-tumorigenic murine fibroblast BALB/3T3 by colorimetric methods using Sulforhodamine B (SRB) and tetrazolium salt (MTT), enzyme caspases 3/7 activity, and cell cycle analysis by flow cytometry. Statistical analysis was performed by the Shapiro–Wilk and Brown–Forsythe tests ensured normality and variance equality, respectively, followed by one-way ANOVA (Dunnett’s and Tukey’s tests) for caspase 3/7 and two-way ANOVA (Dunnett’s test) for cell cycle analysis.

Results

Aristolochia baetica (Aristolochiaceae) and Lavandula dentata (Lamiaceae) extracts have significant antiproliferative properties with caspase 3/7-independent activity which implies that the cytotoxicity of this extracts induces cell death through non-apoptotic mechanisms. Extracts from lavender and aristolochia exhibit antiproliferative effects by disrupting cell cycle progression, leading to the accumulation of dead cells in the sub-G1 phase.

Conclusion

These two species show promising therapeutic potential as antiproliferative agents, operating through mechanisms distinct from those of conventional chemotherapeutic agents.
Language:
English
Publication timeframe:
1 times per year
Journal Subjects:
Life Sciences, Molecular Biology, Microbiology and Virology, Medicine, Basic Medical Science, Immunology
Journal RSS Feed