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Efficacy of medicinal plants from Algeria on cell proliferation: Involvement of caspases 3/7 and cell cycle disorder

Leila Gadouche

Leila Gadouche

  • Laboratory of Natural Bio-Resources, Department of Biology, Faculty of Natural Sciences and Life, Hassiba Benbouali University of ChlefChlef, Algeria
  • TAMAYOUZ Laboratory Endocrinology and Metabolism (LEM)Algier, Algeria
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Gadouche, Leila
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Dagmara Klopotowska

Dagmara Klopotowska

Department of Experimental Oncology, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of SciencesWroclaw, Poland
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Klopotowska, Dagmara
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Joanna Wietrzyk

Joanna Wietrzyk

Department of Experimental Oncology, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of SciencesWroclaw, Poland
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Wietrzyk, Joanna
  
Mar 19, 2025
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Article Category: Original Study
Published Online: Mar 19, 2025
Page range: 16 - 24
Received: Sep 18, 2024
Accepted: Jan 15, 2025
DOI: https://doi.org/10.2478/ahem-2025-0003
Keywords
© 2025 Leila Gadouche et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1.

Caspase-3/7 activity after 72 h of HepG2 cells treatment with extracts at 200 µg/ml concentration. Concentrations of cytostatics: Cisplatin – 1 µg/ml, Doxorubicin – 10 µM, and Camptothecin – 0,5 µg/ml. Doxorubicin and Camptothecin were added 24 h before Caspase-3/7 assay. The normality of the data distribution was performed using the Shapiro-Wilk test. One-way ANOVA tests with Dunnett’s multiple comparisons test were performed. **** - p< 0,0001 vs. ctrl. The number of observations for ctrl and Aristolochia baetica – 7, Lavendula dentata – 6, Cisplatin – 5, Doxorubicin, and Camptothecin – 3.
Caspase-3/7 activity after 72 h of HepG2 cells treatment with extracts at 200 µg/ml concentration. Concentrations of cytostatics: Cisplatin – 1 µg/ml, Doxorubicin – 10 µM, and Camptothecin – 0,5 µg/ml. Doxorubicin and Camptothecin were added 24 h before Caspase-3/7 assay. The normality of the data distribution was performed using the Shapiro-Wilk test. One-way ANOVA tests with Dunnett’s multiple comparisons test were performed. **** - p< 0,0001 vs. ctrl. The number of observations for ctrl and Aristolochia baetica – 7, Lavendula dentata – 6, Cisplatin – 5, Doxorubicin, and Camptothecin – 3.

Figure 2.

The cell cycle analysis for extracts at 1000 µg/ml after 72 hours of treatment of MV4-11 cells. Concentrations of cisplatin – 0.4 µg/ml. The number of observations for ctrl – 5, Aristolochia baetica and Lavendula dentata – 4, Cisplatin – 6. Two-way ANOVA and Dunnett’s multiple comparisons test was used for cell cycle analysis. *- p < 0.05, **- p < 0.01, ***- p < 0.001, ****-p < 0.0001 vs. ctrl
The cell cycle analysis for extracts at 1000 µg/ml after 72 hours of treatment of MV4-11 cells. Concentrations of cisplatin – 0.4 µg/ml. The number of observations for ctrl – 5, Aristolochia baetica and Lavendula dentata – 4, Cisplatin – 6. Two-way ANOVA and Dunnett’s multiple comparisons test was used for cell cycle analysis. *- p < 0.05, **- p < 0.01, ***- p < 0.001, ****-p < 0.0001 vs. ctrl

Figure 3.

The cell cycle analysis for extracts at 100 µg/ml after 72 hours of treatment of MV4-11 cells. Concentrations of cisplatin – 0.4 µg/ml. The number of observations for ctrl – 5, Aristolochia baetica and Lavendula dentata – 4, Cisplatin – 6. Two-way ANOVA and Dunnett’s multiple comparisons test was used for cell cycle analysis. *- p < 0.05, **- p < 0.01, ****-p < 0.0001 vs. ctrl.
The cell cycle analysis for extracts at 100 µg/ml after 72 hours of treatment of MV4-11 cells. Concentrations of cisplatin – 0.4 µg/ml. The number of observations for ctrl – 5, Aristolochia baetica and Lavendula dentata – 4, Cisplatin – 6. Two-way ANOVA and Dunnett’s multiple comparisons test was used for cell cycle analysis. *- p < 0.05, **- p < 0.01, ****-p < 0.0001 vs. ctrl.

The antiproliferative activity of tested extracts presented as the mean IC50 value ± standard deviation (SD)_ The extracts were dissolved in culture medium

A-498 HepG2 PLC/PRF/5 MDA-MB-231 MV4-11 BALB/3T3
IC50 (mean ± SD) [μg/mL]
Aristolochia baetica 430.20±138.23 340.94±143.0 722.25±103.98 241.21±73.38 277.08±87.26 387.69±142.75
Cistus lanifedus 528.65±277.15 759.24±200.31 n.a. 403.77±157.14 n.t. 604.93±315.09
Crataegus Monogyna (Leaves) n.a. n.a. n.a. 598.15±45.96 n.t. n.a.
Crataegus Monogyna (Fruit) n.a. n.a. n.a. 84.99±11.11* n.t. n.a.
Erica arborea 347.27±106.93 481.40±148.33 n.a. 450.48±158.56 n.a. 288.68±11.00
Rhamnus alaternus n.a. 729.25±205.32 n.a. n.a. n.t. n.a.
Lavendula dentata 375.60±87.05 317.38±25.75 354.15±13.99 277.91±24.73 684.34±192.55 226.85±25.63
Cisplatin 2.64+±0.78 1.32±0.94 1.64±0.29 3.11±0.48 0.39±0.047 0.50±0.32

Fold mean relative caspase-3/7 activity in comparison to the untreated control ± SD in HepG2 cells

Mean relative caspase-3/7 activity ± SD
Aristolochia baetica (200μg/ml) 0.680±0.134
Lavendula dentata (200μg/ml) 0.795±0.120
Cisplatin (1μg/ml) 1.425±0.067
Doxorubicin (10μM) 4.890±1.096
Camptothecin (0,5μg/ml) 8.975±0.028
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