<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>This study aimed to prepare a niosomal gel of green tea (<italic>Camellia sinensis</italic>) extract containing catechins, mostly epigallocatechin-3-gallate (ECGC), as a potent antioxidant. Niosomes can increase EGCG's stability and penetration into the skin for a better therapeutic effect. Niosomes were prepared by a thin-layer hydration method, were evaluated for their vesicle shape, particle size, polydispersity index, zeta potential and entrapment efficiency, and then incorporated into gels using sodium alginate as a gelling agent. Three niosomal gel formulations were prepared with different concentrations of niosomes green tea extract. Afterwards, organoleptic properties, chemical and physical characteristics, antioxidant activity, and stability and irritability of the niosomal gels were investigated. The different concentrations of green tea extract had a significant effect on the physical characteristics, but not on the chemical ones. Its antioxidant activity was determined using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging method. The 50% extract green tea niosomes gel showed the highest inhibition value (25.13%). The stability was determined by freeze–thaw and real-time methods; they showed a decrease in pH, but still within the pH range of skin. The irritability test used was the Hen's Egg Test-Chorioallantoic Membrane (HET-CAM) method, which showed no irritation for all formulas. In conclusion, 50% green tea extract niosomes gel results showed it to be the best formulation with optimal antioxidant results.</p>
</abstract>

This study aimed to prepare a niosomal gel of green tea (Camellia sinensis) extract containing catechins, mostly epigallocatechin-3-gallate (ECGC), as a potent antioxidant. Niosomes can increase EGCG's stability and penetration into the skin for a better therapeutic effect. Niosomes were prepared by a thin-layer hydration method, were evaluated for their vesicle shape, particle size, polydispersity index, zeta potential and entrapment efficiency, and then incorporated into gels using sodium alginate as a gelling agent. Three niosomal gel formulations were prepared with different concentrations of niosomes green tea extract. Afterwards, organoleptic properties, chemical and physical characteristics, antioxidant activity, and stability and irritability of the niosomal gels were investigated. The different concentrations of green tea extract had a significant effect on the physical characteristics, but not on the chemical ones. Its antioxidant activity was determined using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging method. The 50% extract green tea niosomes gel showed the highest inhibition value (25.13%). The stability was determined by freeze–thaw and real-time methods; they showed a decrease in pH, but still within the pH range of skin. The irritability test used was the Hen's Egg Test-Chorioallantoic Membrane (HET-CAM) method, which showed no irritation for all formulas. In conclusion, 50% green tea extract niosomes gel results showed it to be the best formulation with optimal antioxidant results.

Thin-layer hydration method to prepare a green tea extract niosomal gel and its antioxidant performance

Department of Pharmacy, Faculty of Health Science

European Pharmaceutical Journal

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

This study aimed to prepare a niosomal gel of green tea (Camellia sinensis) extract containing catechins, mostly epigallocatechin-3-gallate (ECGC), as a potent...

Thin-layer hydration method to prepare a green tea extract niosomal gel and its antioxidant performance

Article Category: Original Paper

Published Online: Oct 13, 2021

Page range: 125 - 135

Received: Dec 14, 2020

Accepted: Jun 29, 2021

DOI: https://doi.org/10.2478/afpuc-2021-0011

Keywords
green tea extract, niosomes gel, antioxidant activity, stability, irritability

© 2021 U. Chasanah et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Thin-layer hydration method to prepare a green tea extract niosomal gel and its antioxidant performance

Article Category: Original Paper

Published Online: Oct 13, 2021

Page range: 125 - 135

Received: Dec 14, 2020

Accepted: Jun 29, 2021

DOI: https://doi.org/10.2478/afpuc-2021-0011

Keywordsgreen tea extract, niosomes gel, antioxidant activity, stability, irritability

© 2021 U. Chasanah et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
green tea extract, niosomes gel, antioxidant activity, stability, irritability