<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Genital mycoplasmosis is a condition present in bovine production systems, and the most important agents involved are <italic>Mycoplasma bovis</italic>, <italic>Mycoplasma bovigenitalium</italic> and <italic>Ureaplasma diversum</italic>. Some aspects of their pathogenesis remain unclear. This study was designed in order to evaluate their ability to stimulate mononuclear cells from the endometrium, vagina and peripheral blood of cycling and healthy cows to produce nitric oxide (NO). Cellular cultures of endometrial, vaginal and peripheral blood cells from 33 healthy cows were cultivated with <italic>Mycoplasma bovis</italic>, <italic>Mycoplasma bovigenitalium</italic> and <italic>Ureaplasma diversum</italic> originated from the 4<sup>th</sup> passage in culture broth and the NO production was measured by the Greiss reaction. Confirmation of the presence of mononuclear cells and of the agents during and after the NO assay was done by Giemsa stained smears and further cultivation and detection by PCR reaction. Mononuclear cells from all samples produced NO. <italic>Mycoplasma bovigenitalium</italic> stimulated higher NO production than the others (p&lt;0.05). Endometrial cells produced less NO than vaginal or blood cultured cells. In conclusion, it seems that <italic>Mycoplasma bovis</italic>, <italic>Mycoplasma bovigenitalium</italic>, and <italic>Ureaplasma diversum</italic> are able to activate mononuclear cells and induce the production of NO, thus suggesting that this pathway is elicited in response to the primary infection by these agents. More studies are necessary to verify why these agents remain in the bovine reproductive tract for long periods and how they reassume deleterious effects.</p>
</abstract>

Genital mycoplasmosis is a condition present in bovine production systems, and the most important agents involved are Mycoplasma bovis, Mycoplasma bovigenitalium and Ureaplasma diversum. Some aspects of their pathogenesis remain unclear. This study was designed in order to evaluate their ability to stimulate mononuclear cells from the endometrium, vagina and peripheral blood of cycling and healthy cows to produce nitric oxide (NO). Cellular cultures of endometrial, vaginal and peripheral blood cells from 33 healthy cows were cultivated with Mycoplasma bovis, Mycoplasma bovigenitalium and Ureaplasma diversum originated from the 4th passage in culture broth and the NO production was measured by the Greiss reaction. Confirmation of the presence of mononuclear cells and of the agents during and after the NO assay was done by Giemsa stained smears and further cultivation and detection by PCR reaction. Mononuclear cells from all samples produced NO. Mycoplasma bovigenitalium stimulated higher NO production than the others (p<0.05). Endometrial cells produced less NO than vaginal or blood cultured cells. In conclusion, it seems that Mycoplasma bovis, Mycoplasma bovigenitalium, and Ureaplasma diversum are able to activate mononuclear cells and induce the production of NO, thus suggesting that this pathway is elicited in response to the primary infection by these agents. More studies are necessary to verify why these agents remain in the bovine reproductive tract for long periods and how they reassume deleterious effects.

Evaluation of <italic>in vitro</italic> Activation of Bovine Endometrial and Vaginal Epithelial and Blood Mononuclear Cells to Produce Nitric Oxide in Response to <italic>Mycoplasma bovis, Mycoplasma bovigenitalium and Ureaplasma diversum</italic>

Institute for Tropical Pathology and Public Health Federal University of Goiás

Acta Veterinaria

This work is licensed under the Creative Commons Attribution 4.0 International License.

Evaluation of  Activation of Bovine Endometrial and Vaginal Epithelial and Blood Mononuclear Cells to Produce Nitric Oxide in Response to 

Genital mycoplasmosis is a condition present in bovine production systems, and the most important agents involved are Mycoplasma bovis, Mycoplasma...

Evaluation of in vitro Activation of Bovine Endometrial and Vaginal Epithelial and Blood Mononuclear Cells to Produce Nitric Oxide in Response to Mycoplasma bovis, Mycoplasma bovigenitalium and Ureaplasma diversum

Published Online: Jul 12, 2021

Page range: 137 - 146

Received: Jan 23, 2020

Accepted: Mar 23, 2021

DOI: https://doi.org/10.2478/acve-2021-0012

Keywords
cow, Genital micoplasmosis, innate immunity, infection

© 2021 Regiani Nascimento Gagno Pôrto et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Evaluation of in vitro Activation of Bovine Endometrial and Vaginal Epithelial and Blood Mononuclear Cells to Produce Nitric Oxide in Response to Mycoplasma bovis, Mycoplasma bovigenitalium and Ureaplasma diversum

Published Online: Jul 12, 2021

Page range: 137 - 146

Received: Jan 23, 2020

Accepted: Mar 23, 2021

DOI: https://doi.org/10.2478/acve-2021-0012

Keywordscow, Genital micoplasmosis, innate immunity, infection

© 2021 Regiani Nascimento Gagno Pôrto et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
cow, Genital micoplasmosis, innate immunity, infection