1. bookVolume 71 (2021): Issue 2 (June 2021)
Journal Details
License
Format
Journal
eISSN
1820-7448
First Published
25 Mar 2014
Publication timeframe
4 times per year
Languages
English
access type Open Access

Evaluation of in vitro Activation of Bovine Endometrial and Vaginal Epithelial and Blood Mononuclear Cells to Produce Nitric Oxide in Response to Mycoplasma bovis, Mycoplasma bovigenitalium and Ureaplasma diversum

Published Online: 12 Jul 2021
Volume & Issue: Volume 71 (2021) - Issue 2 (June 2021)
Page range: 137 - 146
Received: 23 Jan 2020
Accepted: 23 Mar 2021
Journal Details
License
Format
Journal
eISSN
1820-7448
First Published
25 Mar 2014
Publication timeframe
4 times per year
Languages
English
Abstract

Genital mycoplasmosis is a condition present in bovine production systems, and the most important agents involved are Mycoplasma bovis, Mycoplasma bovigenitalium and Ureaplasma diversum. Some aspects of their pathogenesis remain unclear. This study was designed in order to evaluate their ability to stimulate mononuclear cells from the endometrium, vagina and peripheral blood of cycling and healthy cows to produce nitric oxide (NO). Cellular cultures of endometrial, vaginal and peripheral blood cells from 33 healthy cows were cultivated with Mycoplasma bovis, Mycoplasma bovigenitalium and Ureaplasma diversum originated from the 4th passage in culture broth and the NO production was measured by the Greiss reaction. Confirmation of the presence of mononuclear cells and of the agents during and after the NO assay was done by Giemsa stained smears and further cultivation and detection by PCR reaction. Mononuclear cells from all samples produced NO. Mycoplasma bovigenitalium stimulated higher NO production than the others (p<0.05). Endometrial cells produced less NO than vaginal or blood cultured cells. In conclusion, it seems that Mycoplasma bovis, Mycoplasma bovigenitalium, and Ureaplasma diversum are able to activate mononuclear cells and induce the production of NO, thus suggesting that this pathway is elicited in response to the primary infection by these agents. More studies are necessary to verify why these agents remain in the bovine reproductive tract for long periods and how they reassume deleterious effects.

Keywords

1. Lysnyansky I, Brenner AN, Benjamin A, Bernstein M, Elad, D, Blum, S, Friedgut O, Rotemberg D: Identification of Mycoplasma bovigenitalium and Mycoplasma canadense from outbreaks of granulopapular vulvovaginitis in dairy cattle in Israel. Vet Rec. 2009, 165: 319-322.10.1136/vr.165.11.319 Search in Google Scholar

2. Cardoso MV, Scarcelli E, Grasso LMPS, Teixeira SR, Genovez ME: Ureaplasma diversum and reproductive disorder in Brazilian cows and heifer; first report. Anim. Reprod. Sci. 2000. 63: 137-143.10.1016/S0378-4320(00)00176-7 Search in Google Scholar

3. Maunsell FP, Woolums AR, Francoz D, Rosenbusch RF, Step DL, Wilson DJ, Janzen ED: Mycoplasma bovis infections in cattle. J. Vet. Intern. Med. 2011, 25: 772–783.10.1111/j.1939-1676.2011.0750.x21745245 Search in Google Scholar

4. Fox LK, Kirk JH, Britten A: Mycoplasma mastitis: A review of transmission and control. J. Vet. Med. Series B. 2005,52: 153–160.10.1111/j.1439-0450.2005.00845.x16000109 Search in Google Scholar

5. Maunsell F, Brown MB, Powe J, Ivey J, Woolard M, Love W, Simecka JW: Oral inoculation of young dairy calves with Mycoplasma bovis results in colonization of tonsils, development of otitis media and local immunity. PLoS One. 2012, 7: e44523.10.1371/journal.pone.0044523343527522970240 Search in Google Scholar

6. Nascimento-Rocha JM, Oliveira Filho BD, Arnhold E, Pôrto RNG, Lima SF, Gambarini ML: Assessment of cow and farm level risk factors associated with Ureaplasma diversum in pasture-based dairy systems - A field study. An. Acad. Brasil. Ciên. 2017, 89: 1779-1783.10.1590/0001-376520172016081328876387 Search in Google Scholar

7. Pôrto RN G, Oliveira-filho, BD, Ferraz HT, Caixeta LS,, Viu MAO, Gambarini ML: Histopatology of the reproductive tract of Nellore pubertal heifers with genital ureaplasmosis. An. Acad. Bras. Ciênc. 2017, 89:,2987-2996. Search in Google Scholar

8. You X, Zeng Y, Wu Y: Interactions between Mycoplasma lipid-associated membrane proteins and the host cells. J. Z.Univ. SCIENCE B 2006, 7: 342-350.10.1631/jzus.2006.B0342146293016615163 Search in Google Scholar

9. Rottem S: Interaction of Mycoplasmas with host cells. Physiol. Rev. 2003, 83: 417-432.10.1152/physrev.00030.200212663864 Search in Google Scholar

10. Rosseli M, Keller PJ, Debey RK: Role of nitric oxide in the biology, physiology and pathophysiology of reproduction. Hum. Reprod. Update 1998, 4: 3-24.10.1093/humupd/4.1.39622410 Search in Google Scholar

11. Li DJ, Liu YF, Li YF, Lv Y, Pei XY,Guo DZ: Significance of nitric oxide concentration in plasma and uterine secretes with puerperal endometritis in dairy cows. Vet. Res. Comm. 2010,34: 315–21.10.1007/s11259-010-9355-820414720 Search in Google Scholar

12. Song X, Li D J, Feng GF, Li B, Liu YF: Dynamic analysis of nitric oxide and total oxidant capacity in cow uterine secretion with subclinical endometritis. J. N. Agric. Univ. (English Edition) 2015, 22: 35–39.10.1016/S1006-8104(15)30005-2 Search in Google Scholar

13. Lysnyansky I, Ron Y, Yogev,D: Justaposition of an active promoter to vsp genes via site-specific DNA inversions generates antigenic variation in Mycoplasma bovis. J. Bacteriol. 2001,183: 5698-5708.10.1128/JB.183.19.5698-5708.2001 Search in Google Scholar

14. WINSTAT. User’s manual WINSTAT® for EXCEL. R. Fitch Software, Bad Krozingen, 2006,131p. Search in Google Scholar

15. Bogdan C: Nitric oxide and the immune response. Nature Immunol. 2001, 2: 907-916.10.1038/ni1001-907 Search in Google Scholar

16. Jungi TW, Valentin-Weigand P, Brcic M: Differential induction of NO synthesis by Gram-positive and Gram-negative bacteria and their components in bovine monocyte-derived macrophages. Microb. Pathog. 1999, 27: 43-53.10.1006/mpat.1999.0284 Search in Google Scholar

17. Ghanem ME, Higuchi H, Tezuka E, Hideki I, Bhuminand D, Yoshiaki I, Takeshi O: Mycoplasma infection in the uterus of early postpartum dairy cows and its relation to dystocia and endometritis. Theriogenology, 2013,79: 180-185.10.1016/j.theriogenology.2012.09.027 Search in Google Scholar

18. Macêdo AAM, Oliveira JMB, Silva BP, Borges JM, Soares LBF, Silva GM, Santos SB, Mota RA, Pinheiro-Júnior JW: Occurrence of Mycoplasma bovigenitalium and Ureaplasma diversum in dairy cattle from Pernambuco state, Brazil. Arq. Bras. Med. Vet. Zootec. 2018, 70: 1798-1806.10.1590/1678-4162-10132 Search in Google Scholar

19. Petit T, Spergser J, Aurich J, Rosengarten R: Prevalence of Chlamydiaceae and Mollicutes on the genital mucosa and serological findings in dairy cattle. Vet. Microb.2008, 127: 325-333.10.1016/j.vetmic.2007.08.022 Search in Google Scholar

20. Chelmonska-Soyta A, Miller RB, Ruhnke L, Rosendal S: Activation of murine macrophages and lymphocytes by Ureaplasma diversum. Can.J.Vet. Res. 1994.58: 275-280. Search in Google Scholar

21. Chelmonska-Soyta, A, Katska, L, Kurpisz, M, Stefaniak, T, Zimecki, M : The effect of Ureaplasma diversum activated mononuclear leukocytes on the development and interferon-t production by bovine IVF-derived embryos. J. Reprod. Immunol. 2001,51: 145-158.10.1016/S0165-0378(01)00073-0 Search in Google Scholar

22. Marletta MA: Mammalian synthesis of nitrite, nitrate, nitric oxide, and N-nitrosating agents. Chem. Res. Toxicol. 1988,1: 249-257.10.1021/tx00005a0012979740 Search in Google Scholar

23. Cerqueira NF, Yoshida WB: Óxido Nítrico. Revisão. Acta Cir. Bras. 2002. 17, 417-423. Search in Google Scholar

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