1. bookVolume 71 (2021): Issue 2 (June 2021)
Journal Details
License
Format
Journal
First Published
25 Mar 2014
Publication timeframe
4 times per year
Languages
English
access type Open Access

Potential of Inter- and Intra-Species Variability of CHD1 Gene in Birds as a Forensic Tool

Published Online: 12 Jul 2021
Page range: 147 - 157
Received: 01 Apr 2021
Accepted: 14 Jun 2021
Journal Details
License
Format
Journal
First Published
25 Mar 2014
Publication timeframe
4 times per year
Languages
English
Abstract

Nowadays, illegal trafficking and smuggling of animals are among the greatest threats to many avian species. Most commonly smuggled birds belong to parrots, song birds, raptor species and owls. All of these species are protected by national and international legislations. In order to prevent and reduce wildlife trafficking, DNA methods have become an important forensic tool in species and sex identification. In this study, CHD1R/CHD1F primer pair was used to amplify a part of the CHD1 gene from 65 birds that belong to 43 species. For 36 species this is the first time that the length of CHD1 amplicons was measured for the purpose of species determination. The results were visualized using capillary electrophoresis and enabled simultaneous determination of sex and species. Based on the number of amplicons (two in females, one in males) sex was successfully determined in all species, even in cases where gel electrophoresis failed to give results. Moreover, the species was successfully determined in most bird species based on the species-specific sizes of CHD1 amplicon. The method used in this study is of great importance for veterinary forensic medicine and the prevention of wildlife smuggling. Still, further work is necessary to confirm the effectiveness of the method in all bird species.

Keywords

1. Alves RRN, Lima JRDF, Araujo HFP: The live bird trade in Brazil and its conservation implications: an overview. Bird Conserv Int 2013, 23(1):53-65. Search in Google Scholar

2. Jan C, Fumagalli L: Polymorphic DNA microsatellite markers for forensic individual identification and parentage analyses of seven threatened species of parrots (family Psittacidae). PeerJ 2016, 4: e2416. Search in Google Scholar

3. TRAFFIC: An overview of seizures of CITES-listed wildlife in the European Union, 2020. Search in Google Scholar

4. Channing M: Transnational Crime and the Developing World. Global Financial Integrity 2017. Search in Google Scholar

5. Pravilnik o proglašenju i zaštiti strogo zaštićenih i zaštićenih divljih vrsta biljaka, životinja i gljiva (“Sl. glasnik RS”, br. 5/2010 i 47/2011) Search in Google Scholar

6. Weston MK, Memon MA: The illegal parrot trade in Latin America and its consequences to parrot nutrition, health and conservation. Bird Pop 2009, 9: 76-83. Search in Google Scholar

7. Vucicevic M, Stevanov-Pavlovic M, Stevanovic J, Bosnjak J, Gajic B, Aleksic N, Stanimirovic Z: Sex Determination in 58 Bird Species and Evaluation of CHD Gene as a Universal Molecular Marker in Bird Sexing. Zoo Biol 2013, 32:269-276. Search in Google Scholar

8. Bosnjak J, Stevanov-Pavlovic M, Vucicevic M, Stevanovic J, Simeunovic P, Resanovic R, Stanimirovic Z: Feasibility of noninvasive molecular method for sexing of parrots. Pak J Zool 2013, 45:715-720. Search in Google Scholar

9. Stevanov–Pavlovic M, Vucicevic M, Bosnjak J, Stevanovic J, Dimitrijevic V, Resanovic R, Stanimirovic Z: Molecular sex determination of 20 bird species protected in the Republic of Serbia. Acta Vet-Beograd 2013, 63:45-51. Search in Google Scholar

10. Lee JC, Tsai LC, Hwa PY, Chan CL, Huang A, Chin SC, Wang LC, Lin JT, Linacre A, Hsieh HM: A novel strategy for avian species and gender identification using the CHD gene. Mol Cell Probes 2010, 24:27-31. Search in Google Scholar

11. Davitkov D, Glavinic U, Nesic K, Davitkov D, Vucicevic M, Nesic V, Stanimirovic Z: Improved DNA-based identification of Cervidae species in forensic investigations. Acta Vet-Beograd 2017, 67(4):449-458. Search in Google Scholar

12. Dalén L, Lagerholm VK, Nylander JA, Barton N, Bochenski ZM, Tomek T, Rudling D, Ericson PG, Irestedt M, Stewart JR: Identifying bird remains using ancient DNA barcoding. Genes 2017, 6:169. Search in Google Scholar

13. Hebert PDN, Stoeckle MY, Zemlak TS, Francis CM: Identification of Birds through DNA Barcodes. PLoS BIOL 2004, 2 (10):e312. Search in Google Scholar

14. Rudnick JA, Katzner TE, Bragin EA, DeWoody JA: Species identification of birds through genetic analysis of naturally shed feathers. Mol Ecol Notes 2007, 7(5):757-762. Search in Google Scholar

15. Ushio M, Murata K, Sado T, Nishiumi I, Takeshita M, Iwasaki W, Miya M: Demonstration of the potential of environmental DNA as a tool for the detection of avian species. Sci Rep 2018, 14:8(1):1-10. Search in Google Scholar

16. Manthey JD, Campillo LC, Burns KJ, Moyle RG: Comparison of target-capture and restriction-site associated DNA sequencing for phylogenomics: a test in cardinalid tanagers (Aves, Genus: Piranga). Syst Biol 2016, 1:65(4):640-50. Search in Google Scholar

17. Perktaş U, Groth JG, Barrowclough GF: Phylogeography, species limits, phylogeny, and classification of the turacos (Aves: Musophagidae) based on mitochondrial and nuclear DNA sequences. Am Mus Novit 2020, 3949:1-61. Search in Google Scholar

18. Farag M, Imam T, Dhama K: Identification of some domestic animal species (camel, buffalo and sheep) by PCR-RFLP analysis of the mitochondrial cytochrome b gene. Adv Anim Vet Sci 2015, 3(2):136-142. Search in Google Scholar

19. Rahat MA, Haris M, Ullah Z, Ayaz SG, Nouman M, Rasool A, Israr M: Domestic animals’ identification using PCR-RFLP analysis of cytochrome b gene. Adv life sci 2020, 7 (3): 113-116. Search in Google Scholar

20. Klomtong P, Phasuk Y, Duangjinda M: Animal species identification through high resolutionmelting real time PCR (HRM) of the mitochondrial 16S rRNA gene. Ann Anim Sci 2016, 16:415–424. Search in Google Scholar

21. Çakmak E, Akın Pekşen Ç, Bilgin CC: Comparison of three different primer sets for sexing birds. J Vet Diagn Invest 2017, 29:59-63. Search in Google Scholar

22. Morinha F, Cabral JA, Bastos E: Molecular sexing of birds: A comparative review of polymerase chain reaction (PCR)-based methods. Theriogenology 2012, 78:703-714. Search in Google Scholar

23. Centeno-Cuadros A, Abbasi I, Nathan R: Sex determination in the wild: a field application of loop-mediated isothermal amplification successfully determines sex across three raptor species. Mol Ecol Resour 2017, 17:153-160. Search in Google Scholar

24. Rahim A, Kumar S, Sharma D, Kokate LS, Das AK, Singh JA, Kataria MC. Species and gender identification in diversified poultry species by PCR analysis of CHD gene. Indian Journal of Poultry Science 2012, 47(3):281-6. Search in Google Scholar

25. Gill P, Gusmão L, Haned H, Mayr WR, Morling N, Parson W, Prieto L, Prinz M, Schneider H, Schneider PM, Weir BS: DNA commission of the International Society of Forensic Genetics: Recommendations on the evaluation of STR typing results that may include dropout and/or drop-in using probabilistic methods. Forensic Sci Int Genet 2012, 6(6):679-88. Search in Google Scholar

26. De Andrade Silva KVK, Lôbo-Hajdu G, Alves MAS: Sex determination in Turdus amaurochalinus (Passeriformes: Muscicapidae): morphometrical analysis supported by CHD gene. Rev Biol Trop 2011, 59(2):789-794. Search in Google Scholar

27. Khan S, Ijaz J, Hayat M, Zahra T, Raza M, Abbas K: Sex determination by CHD (Chromo helicase DNA binding) gene in local rock pigeons (Columba livia) from Lahore, Pakistan. J Entomol Zool 2019, 7:664-666. Search in Google Scholar

28. Griffiths R, Double MC, Orr K, Dawson RJG: A DNA test to sex most birds. Mol Ecol 1998, 7:1071-1075. Search in Google Scholar

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