<abstract xmlns="http://www.w3.org/1999/xhtml">Panthenol is a biologically active compound closely related to vitamin B5 (pantothenic acid). This work deals with the separation of panthenol enantiomers using high performance liquid chromatography. Different types of chiral stationary phases (β-cyclodextrin, isopropyl carbamate cyclofructan 6, amylose tris(3,5-dimethylphenylcarbamate)) were tested in normal phase separation mode. Effect of mobile phase composition on the resolution and retention of enantiomers was studied. Two types of detectors, low-wavelength UV and polarimetric, were used. The optimal chromatographic system includes a chiral stationary phase based on amylose and a mobile phase of hexane/ethanol (60/40, v/v) where the resolution of enantiomers reached the value <italic>R</italic>s = 2.49. Suitable chromatographic conditions were applied for the determination of panthenol enantiomers in samples of pharmaceutical preparations with the obtained recovery of more than 92 %. Linearity of the high performance liquid chromatography method with spectrophotometric detection was from 1.0 × 10−3 to 1.3 mg mL−1 (<italic>R</italic>2 = 0.998), with the limit of detection of 0.3 × 10−3 mg mL−1 for both enantiomers.</abstract>

Panthenol is a biologically active compound closely related to vitamin B5 (pantothenic acid). This work deals with the separation of panthenol enantiomers using high performance liquid chromatography. Different types of chiral stationary phases (β-cyclodextrin, isopropyl carbamate cyclofructan 6, amylose tris(3,5-dimethylphenylcarbamate)) were tested in normal phase separation mode. Effect of mobile phase composition on the resolution and retention of enantiomers was studied. Two types of detectors, low-wavelength UV and polarimetric, were used. The optimal chromatographic system includes a chiral stationary phase based on amylose and a mobile phase of hexane/ethanol (60/40, v/v) where the resolution of enantiomers reached the value Rs = 2.49. Suitable chromatographic conditions were applied for the determination of panthenol enantiomers in samples of pharmaceutical preparations with the obtained recovery of more than 92 %. Linearity of the high performance liquid chromatography method with spectrophotometric detection was from 1.0 × 10−3 to 1.3 mg mL−1 (R2 = 0.998), with the limit of detection of 0.3 × 10−3 mg mL−1 for both enantiomers.

HPLC separation of panthenol enantiomers on different types of chiral stationary phases

Slovak University of Technology in Bratislava

Faculty of Chemical and Food Technology, Institute of Analytical Chemistry

Acta Chimica Slovaca

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Panthenol is a biologically active compound closely related to vitamin B5 (pantothenic acid). This work deals with the separation of panthenol enantiomers...

HPLC separation of panthenol enantiomers on different types of chiral stationary phases

Published Online: Dec 19, 2018

Page range: 114 - 119

DOI: https://doi.org/10.2478/acs-2018-0017

Keywords
chiral stationary phase, HPLC, panthenol, pharmaceutical preparations, polarimetric detection

© 2018 Anna Lomenova et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

HPLC separation of panthenol enantiomers on different types of chiral stationary phases

Published Online: Dec 19, 2018

Page range: 114 - 119

DOI: https://doi.org/10.2478/acs-2018-0017

Keywordschiral stationary phase, HPLC, panthenol, pharmaceutical preparations, polarimetric detection

© 2018 Anna Lomenova et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
chiral stationary phase, HPLC, panthenol, pharmaceutical preparations, polarimetric detection