<abstract xmlns="http://www.w3.org/1999/xhtml">A novel and simple method for the determination of penicillamine (PEN), tiopronin (mercaptopropionyl glycine, MPG) and glutathione (GSH) in pharmaceutical formulations by kinetic spectrophotometry has been developed and validated. It is based on the redox reaction where the thiol compound (RSH) reduces CuII-neocuproine complex to CuI-neocuproine complex. The non-steady state signal of the formed CuI- neocuproine complex is measured at 458 nm. The initial rate and fixed time (at 1 min) methods were validated. The calibration graph was linear in the concentration range from 8.0 × 10‒7 to 8.0 × 10‒5 mol L−1 for the initial rate method and from 6.0 × 10‒7 to 6.0 × 10−5 mol L−1 for the fixed time method, with the detection limits of 2.4 × 10−7 and 1.4 × 10‒7 mol L−1, resp. Levels of PEN, MPG and GSH in pharmaceutical formulations were successfully assayed by both methods. The advantages of the presented methods include sensitivity, short analysis time, ease of application and low cost.</abstract>

A novel and simple method for the determination of penicillamine (PEN), tiopronin (mercaptopropionyl glycine, MPG) and glutathione (GSH) in pharmaceutical formulations by kinetic spectrophotometry has been developed and validated. It is based on the redox reaction where the thiol compound (RSH) reduces CuII-neocuproine complex to CuI-neocuproine complex. The non-steady state signal of the formed CuI- neocuproine complex is measured at 458 nm. The initial rate and fixed time (at 1 min) methods were validated. The calibration graph was linear in the concentration range from 8.0 × 10‒7 to 8.0 × 10‒5 mol L−1 for the initial rate method and from 6.0 × 10‒7 to 6.0 × 10−5 mol L−1 for the fixed time method, with the detection limits of 2.4 × 10−7 and 1.4 × 10‒7 mol L−1, resp. Levels of PEN, MPG and GSH in pharmaceutical formulations were successfully assayed by both methods. The advantages of the presented methods include sensitivity, short analysis time, ease of application and low cost.

Determination of penicillamine, tiopronin and glutathione in pharmaceutical formulations by kinetic spectrophotometry

Department of Analytical Chemistry, Faculty of Chemistry and Technology

Acta Pharmaceutica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

A novel and simple method for the determination of penicillamine (PEN), tiopronin (mercaptopropionyl glycine, MPG) and glutathione (GSH) in pharmaceutical...

Determination of penicillamine, tiopronin and glutathione in pharmaceutical formulations by kinetic spectrophotometry

Published Online: Apr 03, 2021

Page range: 619 - 630

Accepted: Oct 31, 2020

DOI: https://doi.org/10.2478/acph-2021-0038

Keywords
penicillamine, tiopronin, glutathione, kinetic spectrophotometry, pharmaceutical formulations

© 2021 Lea Kukoc-Modun et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Determination of penicillamine, tiopronin and glutathione in pharmaceutical formulations by kinetic spectrophotometry

Published Online: Apr 03, 2021

Page range: 619 - 630

Accepted: Oct 31, 2020

DOI: https://doi.org/10.2478/acph-2021-0038

Keywordspenicillamine, tiopronin, glutathione, kinetic spectrophotometry, pharmaceutical formulations

© 2021 Lea Kukoc-Modun et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
penicillamine, tiopronin, glutathione, kinetic spectrophotometry, pharmaceutical formulations