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Analysis of TGFB1, CD105 and FSP1 expression in human granulosa cells during a 7-day primary in vitro culture

Artur Bryja

Artur Bryja

Department of Anatomy, Poznań University of Medical SciencePoznań, Poland
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Bryja, Artur
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Wojciech Pieńkowski

Wojciech Pieńkowski

Division of Perinatology and Women’s Diseases, Poznań University of Medical SciencesPoznań, Poland
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Pieńkowski, Wojciech
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Katarzyna Stefańska

Katarzyna Stefańska

Department of Histology and Embryology, Poznań University of Medical SciencePoznań, Poland
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Stefańska, Katarzyna
, 
Błażej Chermuła

Błażej Chermuła

Division of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznań University of Medical SciencesPoznań, Poland
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Chermuła, Błażej
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Rut Bryl

Rut Bryl

Department of Anatomy, Poznań University of Medical SciencePoznań, Poland
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Bryl, Rut
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Maria Wieczorkiewicz

Maria Wieczorkiewicz

Department of Basic and Preclinical Sciences, Institute of Veterinary Medicine, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in ToruńToruń, Poland
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Wieczorkiewicz, Maria
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Jakub Kulus

Jakub Kulus

Department of Diagnostics and Clinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University in ToruńToruń, Poland
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Kulus, Jakub
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Grzegorz Wąsiatycz

Grzegorz Wąsiatycz

Department of Veterinary Surgery, Institute of Veterinary Medicine, Nicolaus Copernicus University in ToruńToruń, Poland
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Wąsiatycz, Grzegorz
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Dorota Bukowska

Dorota Bukowska

Department of Diagnostics and Clinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University in ToruńToruń, Poland
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Bukowska, Dorota
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Kornel Ratajczak

Kornel Ratajczak

Department of Veterinary Surgery, Institute of Veterinary Medicine, Nicolaus Copernicus University in ToruńToruń, Poland
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Ratajczak, Kornel
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Jędrzej M. Jaśkowski

Jędrzej M. Jaśkowski

Department of Diagnostics and Clinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University in ToruńToruń, Poland
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Jaśkowski, Jędrzej M.
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James N. Petitte

James N. Petitte

Prestage Department of Poultry Science, North Carolina State UniversityRaleigh, NC, USA
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Petitte, James N.
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Paul Mozdziak

Paul Mozdziak

  • Prestage Department of Poultry Science, North Carolina State UniversityRaleigh, NC, USA
  • Physiology Graduate Program, North Carolina State UniversityRaleigh, NC, USA
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Mozdziak, Paul
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Leszek Pawelczyk

Leszek Pawelczyk

Division of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznań University of Medical SciencesPoznań, Poland
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Pawelczyk, Leszek
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Robert Z. Spaczyński

Robert Z. Spaczyński

Division of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznań University of Medical SciencesPoznań, Poland
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Spaczyński, Robert Z.
 and 
Paweł Antosik

Paweł Antosik

Department of Diagnostics and Clinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University in ToruńToruń, Poland
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Antosik, Paweł
  
Dec 31, 2020
Analysis of TGFB1, CD105 and FSP1 expression in human granulosa cells during a 7-day primary in vitro culture's Cover Image
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Published Online: Dec 31, 2020
Page range: 152 - 157
Received: Oct 06, 2020
Accepted: Nov 17, 2020
DOI: https://doi.org/10.2478/acb-2020-0019
Keywords
© 2020 Artur Bryja, Wojciech Pieńkowski, Katarzyna Stefańska, Błażej Chermuła, Rut Bryl, Maria Wieczorkiewicz, Jakub Kulus, Grzegorz Wąsiatycz, Dorota Bukowska, Kornel Ratajczak, Jędrzej M. Jaśkowski, James N. Petitte, Paul Mozdziak, Leszek Pawelczyk, Robert Z. Spaczyński, Paweł Antosik, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

TGFB 1, CD105 and FSP1 expression in granulosa cells during 7-day in vitro culture. Data was presented like logarithm of fold change. Statistical analysis of the data was performed using the T-test and the differences were considered to be significant at the level of p<0.001 (***), p<0.01 (**), p<0.05 (*)
TGFB 1, CD105 and FSP1 expression in granulosa cells during 7-day in vitro culture. Data was presented like logarithm of fold change. Statistical analysis of the data was performed using the T-test and the differences were considered to be significant at the level of p<0.001 (***), p<0.01 (**), p<0.05 (*)

Oligonucleotide sequences of primers used for RT-qPCR analysis

GENEGENE ACCESSION NUMBERPRIMER SEQUENCE (5’-3’)PRODUCT SIZE (BP)
TGFB1NM_000660CCTTGCGGAAGTCAATGTACA GGCCTTTCCTGCTTCTCATG150
CD(ENG) 105BC014271CACTAGCCAGGTCTCGAAGG CTGAGGACCAGAAGCACCTC165
(SFSP1001 A4)CR450345TCTTGGGGAAAAGGACAGATGA CCCAACCACATCAGAGGAGT195
ACTBNM_001101CTCAGGAGGAGCAATGATCTTG AAAGACCTGTACGCCAACAC132
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English
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Journal Subjects:
Life Sciences, Molecular Biology, Biochemistry
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