1. bookVolume 1 (2017): Issue 1 (January 2017)
Journal Details
License
Format
Journal
eISSN
2564-615X
First Published
30 Jan 2017
Publication timeframe
4 times per year
Languages
English
access type Open Access

Antibody engineering and phage display technology

Published Online: 27 Jan 2017
Volume & Issue: Volume 1 (2017) - Issue 1 (January 2017)
Page range: 95 - 96
Received: 27 Jan 2017
Journal Details
License
Format
Journal
eISSN
2564-615X
First Published
30 Jan 2017
Publication timeframe
4 times per year
Languages
English
Abstract

Here we provide our pipeline for antibody discovery process. Starting from de novo isolation from custom made phage display libraries to expression and downstream applications; it allows us to obtain recombinant antibodies with desired properties to vast set of antigens. We also developed the CYB5-fusion system for periplasmic expression of multimeric proteins with the possibility of process monitoring. The combination of redox dependent absorbance spectrum of red-colored cytochrome b5 with its high value molar extinction coefficient allows us to monitor antibody fusion proteins localization, redox state and quantify them in reliable way in turbid solutions. Moreover, it was revealed that due to outstanding stability and solubility, cytochrome b5 significantly enhances expression level of antibody fragments in Escherichia coli periplasm.

1. J. Cousin-Frankel. Cancer Immunotherapy. Science 2013; 342:1432-1433.10.1126/science.342.6165.1432Search in Google Scholar

2. Paduch M, et al. Generating conformation-specific synthetic antibodies to trap proteins in selected functional states. Methods 2013; 60(1): 3-14.10.1016/j.ymeth.2012.12.010Search in Google Scholar

3. Dormeshkin DO, et al. Development of CYB5-fusion monitoring system for efficient periplasmic expression of multimeric proteins in Escherichia coli. Protein Expr Purif 2016; 128:60-6610.1016/j.pep.2016.08.007Search in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo