1. bookVolume 1 (2017): Issue 1 (January 2017)
Journal Details
License
Format
Journal
eISSN
2564-615X
First Published
30 Jan 2017
Publication timeframe
4 times per year
Languages
English
access type Open Access

Culture cells in a model of microgravity

Published Online: 27 Jan 2017
Volume & Issue: Volume 1 (2017) - Issue 1 (January 2017)
Page range: 93 - 94
Received: 27 Jan 2017
Journal Details
License
Format
Journal
eISSN
2564-615X
First Published
30 Jan 2017
Publication timeframe
4 times per year
Languages
English
Abstract

The main objective of the work was to clarify the question - how will cell cultures functional state change after microgravity simulation when the shift in full strength direction takes place? Proliferation processes and apoptosis intensity in cell lines of rat glioma and human fibroblasts were compared in changing the position of flasks with cell culture in relation to the horizon. The detection of apoptosis and necrosis processes was carried out using flow cytofluorimetry. It was found that the change in full strength direction provides an inhibitory effect on tumor glial cells and fibroblasts’ proliferative activity enhances along with inhibition of apoptotic processes. Intensification of apoptotic processes in glioma cells and attenuation of cell death processes in normal cells - fibroblasts - are the result of cell cooperation disturbance.

1. Teacher’s Guide to Plant Experiments in Microgravity. United Nations. 2013; 45p. Available at http://www.unoosa.org/pdf/sap/hsti/ZGIP/Teachers_Guide_Plant_L.pdf.Search in Google Scholar

2. Khotianovich M, Stukach Yu, Navasiolava N, Custaud M.A, Kulchitsky V. Glial cells and fibroblasts cooperation and viability in simulated microgravity in vitro. Activitas Nervosa Superior Rediviva 2015; 57(1-2):16-21.Search in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo