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Morphological and molecular characterization of Paratylenchus beltsvillensis n. sp. (Tylenchida: Paratylenchidae) from the rhizosphere of pine tree (Pinus virginiana Mill) in Maryland, USA

Mihail R. Kantor

Mihail R. Kantor

Mycology and Nematology Genetic Diversity and Biology LaboratoryBeltsville, USA
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Kantor, Mihail R.
, 
Zafar A. Handoo

Zafar A. Handoo

Mycology and Nematology Genetic Diversity and Biology LaboratoryBeltsville, USA
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Handoo, Zafar A.
, 
Sergei A. Subbotin

Sergei A. Subbotin

  • Plant Pest Diagnostic Center, California Department of Food and AgricultureSacramento, USA
  • Center of Parasitology of A.N. Severtsov Institute of Ecology and Evolution of the Russian Academy of SciencesMoscow, Russia
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Subbotin, Sergei A.
, 
Gary R. Bauchan

Gary R. Bauchan

Electron and Confocal MicroscopyBeltsville, USA
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Bauchan, Gary R.
 and 
Joseph D. Mowery

Joseph D. Mowery

Electron and Confocal MicroscopyBeltsville, USA
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Mowery, Joseph D.
  
Sep 07, 2021
Morphological and molecular characterization of Paratylenchus beltsvillensis n. sp. (Tylenchida: Paratylenchidae) from the rhizosphere of pine tree (Pinus virginiana Mill) in Maryland, USA's Cover Image
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Published Online: Sep 07, 2021
Page range: 1 - 10
DOI: https://doi.org/10.21307/jofnem-2021-079
Keywords
© 2021 Mihail R. Kantor et al., published by Sciendo.
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1:

Line drawings of Paratylenchus beltsvillensis n. sp. A: Female pharyngeal region; B: Vulval region with vulva, uterus, and spermatheca; C: Female lip region with stylet; D: Female specimen with vulval opening; E: Lateral field (mid-body); F: Male specimen with spicules and gubernaculum; G–I: Female tails with vulval opening and tail variations; J: Male tale showing spicules and gubernaculum.
Line drawings of Paratylenchus beltsvillensis n. sp. A: Female pharyngeal region; B: Vulval region with vulva, uterus, and spermatheca; C: Female lip region with stylet; D: Female specimen with vulval opening; E: Lateral field (mid-body); F: Male specimen with spicules and gubernaculum; G–I: Female tails with vulval opening and tail variations; J: Male tale showing spicules and gubernaculum.

Figure 2:

Photomicrographs of Paratylenchus beltsvillensis n. sp. A–E: Female anterior ends; F: Male anterior end; G–J: Female posterior ends with vulva area (arrows) and tails; K: Lateral field (mid-body); L: Male posterior end with spicules; M: Whole female specimen (arrow pointing to vulva).
Photomicrographs of Paratylenchus beltsvillensis n. sp. A–E: Female anterior ends; F: Male anterior end; G–J: Female posterior ends with vulva area (arrows) and tails; K: Lateral field (mid-body); L: Male posterior end with spicules; M: Whole female specimen (arrow pointing to vulva).

Figure 3:

SEM images of Paratylenchus beltsvillensis n. sp. A: Female specimen, head; B: Lateral field (mid-body); C: Female specimen, arrow showing the vulva opening; D: Female posterior end, arrow showing the anal opening; E: Whole female specimen with arrows showing vulval and anal openings.
SEM images of Paratylenchus beltsvillensis n. sp. A: Female specimen, head; B: Lateral field (mid-body); C: Female specimen, arrow showing the vulva opening; D: Female posterior end, arrow showing the anal opening; E: Whole female specimen with arrows showing vulval and anal openings.

Figure 4:

Phylogenetic relationships of Paratylenchus beltsvillensis n. sp. with other related species. Bayesian 50% majority rule consensus tree from two runs as inferred from analysis of the D2-D3 of 28S rRNA gene sequence alignment under the GTR + I + G model. Posterior probabilities equal or more than 70% are given for appropriate clades. New sequences are indicated in bold. Clade numbers are given according to Singh et al. (2021). *identified as Paratylenchus sp. in the GenBank.
Phylogenetic relationships of Paratylenchus beltsvillensis n. sp. with other related species. Bayesian 50% majority rule consensus tree from two runs as inferred from analysis of the D2-D3 of 28S rRNA gene sequence alignment under the GTR + I + G model. Posterior probabilities equal or more than 70% are given for appropriate clades. New sequences are indicated in bold. Clade numbers are given according to Singh et al. (2021). *identified as Paratylenchus sp. in the GenBank.

Figure 5:

Phylogenetic relationships of Paratylenchus beltsvillensis n. sp. with other related species: Bayesian 50% majority rule consensus tree from two runs as inferred from analysis of the ITS rRNA gene sequence alignment under the GTR + I + G model. Posterior probabilities equal or more than 70% are given for appropriate clades. New sequences are indicated in bold. Clade numbers are given according to Singh et al. (2021).
Phylogenetic relationships of Paratylenchus beltsvillensis n. sp. with other related species: Bayesian 50% majority rule consensus tree from two runs as inferred from analysis of the ITS rRNA gene sequence alignment under the GTR + I + G model. Posterior probabilities equal or more than 70% are given for appropriate clades. New sequences are indicated in bold. Clade numbers are given according to Singh et al. (2021).

Morphometrics of Paratylenchus beltsvillensis n_ sp_

Character Holotype Female Male
n 1 10 3
L 257.0 252.2 ± 8.18 (245.0–267.0) 275.0 ± 5.5 (270.0–280.0)
a 20 18.43 ± 0.6 (18.0–19.0) 11.0 ± 0.02 (10.98–11.02)
b 2.25 2.12 ± 0.07 (1.99–2.23) 3.2 ± 0.1 (3.1–3.2)
c 12.85 11.06 ± 1.63 (9.92–13.61) 14.43 ± 1.25 (13.0–15.3)
Max. body diam. 13.0 13.70 ± 0.67 (13.0–15.0) 25.0 ± 0.5 (24.5–25.5)
Stylet length 70.0 72.25 ± 2.49 (70.0–75.0) –
Ant end to Ex. Pore 85.0 95.10 ± 4.72 (90.0–100.0) –
Head to gland tip 114.0 118.80 ± 4.10 (115.0–127.0) 85.0 ± 5.0 (80.0–90.0)
Tail length 20.0 23.19 ± 3.09 (17.0–27.5) –
V% 71.0 71.85 ± 1.07 (70.4–73.4) –
Spicules – – 18.3 ± 1.53 (17.0–20.0)
Gubernaculum – – 5.17 ± 0.3 (5.0–5.5)
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