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Morphological and molecular characterization of Paratylenchus beltsvillensis n. sp. (Tylenchida: Paratylenchidae) from the rhizosphere of pine tree (Pinus virginiana Mill) in Maryland, USA

Mihail R. Kantor
Mihail R. Kantor
, 
Zafar A. Handoo
Zafar A. Handoo
, 
Sergei A. Subbotin
Sergei A. Subbotin
, 
Gary R. Bauchan
Gary R. Bauchan
 et 
Joseph D. Mowery
Joseph D. Mowery
   | 07 sept. 2021
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Publié en ligne: 07 sept. 2021
Pages: 1 - 10
DOI: https://doi.org/10.21307/jofnem-2021-079
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© 2021 Mihail R. Kantor et al., published by Sciendo.
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1:

Line drawings of Paratylenchus beltsvillensis n. sp. A: Female pharyngeal region; B: Vulval region with vulva, uterus, and spermatheca; C: Female lip region with stylet; D: Female specimen with vulval opening; E: Lateral field (mid-body); F: Male specimen with spicules and gubernaculum; G–I: Female tails with vulval opening and tail variations; J: Male tale showing spicules and gubernaculum.
Line drawings of Paratylenchus beltsvillensis n. sp. A: Female pharyngeal region; B: Vulval region with vulva, uterus, and spermatheca; C: Female lip region with stylet; D: Female specimen with vulval opening; E: Lateral field (mid-body); F: Male specimen with spicules and gubernaculum; G–I: Female tails with vulval opening and tail variations; J: Male tale showing spicules and gubernaculum.

Figure 2:

Photomicrographs of Paratylenchus beltsvillensis n. sp. A–E: Female anterior ends; F: Male anterior end; G–J: Female posterior ends with vulva area (arrows) and tails; K: Lateral field (mid-body); L: Male posterior end with spicules; M: Whole female specimen (arrow pointing to vulva).
Photomicrographs of Paratylenchus beltsvillensis n. sp. A–E: Female anterior ends; F: Male anterior end; G–J: Female posterior ends with vulva area (arrows) and tails; K: Lateral field (mid-body); L: Male posterior end with spicules; M: Whole female specimen (arrow pointing to vulva).

Figure 3:

SEM images of Paratylenchus beltsvillensis n. sp. A: Female specimen, head; B: Lateral field (mid-body); C: Female specimen, arrow showing the vulva opening; D: Female posterior end, arrow showing the anal opening; E: Whole female specimen with arrows showing vulval and anal openings.
SEM images of Paratylenchus beltsvillensis n. sp. A: Female specimen, head; B: Lateral field (mid-body); C: Female specimen, arrow showing the vulva opening; D: Female posterior end, arrow showing the anal opening; E: Whole female specimen with arrows showing vulval and anal openings.

Figure 4:

Phylogenetic relationships of Paratylenchus beltsvillensis n. sp. with other related species. Bayesian 50% majority rule consensus tree from two runs as inferred from analysis of the D2-D3 of 28S rRNA gene sequence alignment under the GTR + I + G model. Posterior probabilities equal or more than 70% are given for appropriate clades. New sequences are indicated in bold. Clade numbers are given according to Singh et al. (2021). *identified as Paratylenchus sp. in the GenBank.
Phylogenetic relationships of Paratylenchus beltsvillensis n. sp. with other related species. Bayesian 50% majority rule consensus tree from two runs as inferred from analysis of the D2-D3 of 28S rRNA gene sequence alignment under the GTR + I + G model. Posterior probabilities equal or more than 70% are given for appropriate clades. New sequences are indicated in bold. Clade numbers are given according to Singh et al. (2021). *identified as Paratylenchus sp. in the GenBank.

Figure 5:

Phylogenetic relationships of Paratylenchus beltsvillensis n. sp. with other related species: Bayesian 50% majority rule consensus tree from two runs as inferred from analysis of the ITS rRNA gene sequence alignment under the GTR + I + G model. Posterior probabilities equal or more than 70% are given for appropriate clades. New sequences are indicated in bold. Clade numbers are given according to Singh et al. (2021).
Phylogenetic relationships of Paratylenchus beltsvillensis n. sp. with other related species: Bayesian 50% majority rule consensus tree from two runs as inferred from analysis of the ITS rRNA gene sequence alignment under the GTR + I + G model. Posterior probabilities equal or more than 70% are given for appropriate clades. New sequences are indicated in bold. Clade numbers are given according to Singh et al. (2021).

Morphometrics of Paratylenchus beltsvillensis n. sp.

Character Holotype Female Male
n 1 10 3
L 257.0 252.2 ± 8.18 (245.0–267.0) 275.0 ± 5.5 (270.0–280.0)
a 20 18.43 ± 0.6 (18.0–19.0) 11.0 ± 0.02 (10.98–11.02)
b 2.25 2.12 ± 0.07 (1.99–2.23) 3.2 ± 0.1 (3.1–3.2)
c 12.85 11.06 ± 1.63 (9.92–13.61) 14.43 ± 1.25 (13.0–15.3)
Max. body diam. 13.0 13.70 ± 0.67 (13.0–15.0) 25.0 ± 0.5 (24.5–25.5)
Stylet length 70.0 72.25 ± 2.49 (70.0–75.0) –
Ant end to Ex. Pore 85.0 95.10 ± 4.72 (90.0–100.0) –
Head to gland tip 114.0 118.80 ± 4.10 (115.0–127.0) 85.0 ± 5.0 (80.0–90.0)
Tail length 20.0 23.19 ± 3.09 (17.0–27.5) –
V% 71.0 71.85 ± 1.07 (70.4–73.4) –
Spicules – – 18.3 ± 1.53 (17.0–20.0)
Gubernaculum – – 5.17 ± 0.3 (5.0–5.5)
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