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First report of Pratylenchus vulnus associated with apple in Tunisia

Noura Chihani-Hammas
Noura Chihani-Hammas
, 
Lobna Hajji-Hedfi
Lobna Hajji-Hedfi
, 
Hajer Regaieg
Hajer Regaieg
, 
Asma Larayedh
Asma Larayedh
, 
Ahmed Badiss
Ahmed Badiss
, 
Yu Qing
Yu Qing
 and 
Horrigue-Raouani Najet
Horrigue-Raouani Najet
   | Dec 03, 2018
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Published Online: Dec 03, 2018
Page range: 579 - 586
DOI: https://doi.org/10.21307/jofnem-2018-056
Keywords
© 2018 Noura Chihani-Hammas et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1

Photo micrographs of Pratylenchus vulnus specimens: females (B) and males (A), (C): lateral field at mid – body; (D): spermatheca; (E): pharyngeal region; (F) and (H): female tails; (G): vulval region; (I): male tail (Scale bars: A, B = 100 mm; C–I = 20 mm).
Photo micrographs of Pratylenchus vulnus specimens: females (B) and males (A), (C): lateral field at mid – body; (D): spermatheca; (E): pharyngeal region; (F) and (H): female tails; (G): vulval region; (I): male tail (Scale bars: A, B = 100 mm; C–I = 20 mm).

Figure 2

SEM micrograph of P. vulnus male morphology from Tunisia (A, B, C, D): anterior region, (E, F) : tail, (G) : lateral fields; sp : spicules, lig : 4 lateral lines ; anx : 3 labial annuli).
SEM micrograph of P. vulnus male morphology from Tunisia (A, B, C, D): anterior region, (E, F) : tail, (G) : lateral fields; sp : spicules, lig : 4 lateral lines ; anx : 3 labial annuli).

Figure 3

SEM micrograph of P. vulnus female morphology from Tunisia (A; B; C): anterior region, (D): lateral lines at vulva region, (E): vulva, (F, G): tail, (H): lateral fields).
SEM micrograph of P. vulnus female morphology from Tunisia (A; B; C): anterior region, (D): lateral lines at vulva region, (E): vulva, (F, G): tail, (H): lateral fields).

Figure 4

Phylogenetic relationships between Pratylenchus vulnus identified in Tunisia and other Pratylenchus already deposited in GenBank. The Pratylenchus sequences aligned in MEGA with CLUSTALW and the phylogenetic tree made by MEGA version 7.0 based on the Neighbor-Joining method as inferred from partial 18S rRNA gene.
Phylogenetic relationships between Pratylenchus vulnus identified in Tunisia and other Pratylenchus already deposited in GenBank. The Pratylenchus sequences aligned in MEGA with CLUSTALW and the phylogenetic tree made by MEGA version 7.0 based on the Neighbor-Joining method as inferred from partial 18S rRNA gene.

Figure 5

Phylogenetic relationships between Pratylenchus vulnus identified in Tunisia and other Pratylenchus already deposited in GenBank. The Pratylenchus sequences aligned in MEGA with CLUSTALW and the phylogenetic tree made by MEGA version 7.0 based on the Neighbor-Joining method as inferred from D2-D3 expansion segments of the 28S rRNA.
Phylogenetic relationships between Pratylenchus vulnus identified in Tunisia and other Pratylenchus already deposited in GenBank. The Pratylenchus sequences aligned in MEGA with CLUSTALW and the phylogenetic tree made by MEGA version 7.0 based on the Neighbor-Joining method as inferred from D2-D3 expansion segments of the 28S rRNA.

Morphometrics of Pratylenchus vulnus from apples stoolbeds, nurseries (males and females), and orchards from four Tunisian regions (Kasserin, Kairouan, Monastir, and Zaghouan, Tunisia).

Stoolbed
Locality MM106 Chiha
Characters Females Males
n 5 5
L 444.4 ± 29.36 (415.71–485.86) 419.83 ± 32.43 (395.01–475.2)
Stylet length (µm) 14.29± 0.16 (14.17–14.55) 14.85 ± 0.21 (14.56–15.1)
Spicules (µm) 16.88± 0.26 (16.58–17.2)
Gubernaculum (µm) 4.8± 0.39 (4.2–5.25)
V or T 81.22± 0.73 (80.55–82.39) 34.02± 1.17 (32.11–35.2)
a 26.73 ± 1.32 (24.59–28.1) 28.99 ± 2.76 (25.2–31.4)
b 7.62 ± 0.55 (6.87–8.16) 6.72 ± 0.66 (5.88–7.5)
b′ 4.92 ± 0.9 (3.76–5.66) 4.85 ± 0.27 (4.55–5.22)
c 19.11 ± 0.44 (18.57–19.5) 20.75 ± 0.55 (19.88–21.3)
c′ 2.29 ± 0.22 (2.01–2.58) 2.14 ± 0.1 (2.01–2.28)
Nursery females
Locality Lorca Chiha Meski SMCCSPS
Characters Females Females
n 5 5
L 556.71 ± 33.21 (521.32–607.49) 512.48 ± 51.46 (450.11–578.12)
Stylet length (µm) 15. 45± 0.31 (15.17–15.93) 14. 44± 0.6 (13.69–15.19)
Spicules (µm)
Gubernaculum (µm)
V or T 78.77± 1.31 (76.95–80.55) 79.14 ± 1.74 (76.58–81.3)
a 28.8 ± 4.12 (25.93–35.73) 28.88 ± 3.72 (25.72–35.33)
b 7.82 ± 1.33 (6.1–9.56) 7.97 ± 0.84 (7.12–9.18)
b′ 5.26 ± 0.11 (5.16–5.41) 4.27 ± 0.27 (3.88–4.55)
c 20.22 ± 1.91 (18.17–22.7) 21.79 ± 2.81 (18.68–25.5)
c′ 2.44 ± 0.27 (2.08–2.78) 2.33 ± 0.33 (2.04–2.91)
Nursery males
Locality Lorca Chiha Anna Manzel Nour Meski SMCCSPS
Characters Males Males Males
n 5 5 5
L 483.75 ± 10.58 (468.63–492.7) 478.62 ± 30.09 (431.65–503.93) 485.52 ± 34.38(451.12–540.76)
Stylet length (µm) 14.81 ± 0.49 (14.41–15.66) 14.46 ± 0.75 (13.49–15.26) 14.31 ± 0.28 (14.01–14.66)
Spicules (µm) 17.07± 1.35 (15.47–19.14) 19.16± 0.61 (18.25–19.65) 18.15 ± 0.58 (17.37–19)
Gubernaculum (µm) 4.92± 0.34 (4.46–5.31) 5.26± 0.27 (4.95–5.66) 5.07 ± 0.43 (4.56–5.72)
V or T 35.38± 2.75 (32–38.17) 37.56± 2.55 (33.72–40.6) 36.42 ± 2.76 (34.05–40.54)
a 28.85 ± 1.45 (26.5–30.3) 29.37 ± 2.33 (25.87–32.15) 27.01 ± 1.37 (25.36–28.91)
b 8.24 ± 1.21 (7.23–10.22) 9.11 ± 0.38 (8.66–9.67) 9.2 ± 1.01 (8.14–10.5)
b′ 5.31 ± 0.1 (5.2–5.43) 4.29 ± 0.45 (3.77–4.95) 4.85 ± 0.53 (4.47–5.75)
c 19.89 ± 1.48 (17.86–21.62) 20.95 ± 1.46 (18.7–22.47) 20.16 ± 1.83 (18.85–23.37)
c′ 2.29 ± 0.18 (2.09–2.51) 2.06 ± 0.08 (2–2.21) 2.26 ± 0.21 (1.92–2.45)
Orchads
Locality Lorca Chiha Anna Manzel Nour Meski SMCCSPS
Characters Males Males Males
n 5 5 5
L 483.75 ± 10.58 (468.63–492.7) 478.62 ± 30.09 (431.65–503.93) 485.52 ± 34.38(451.12–540.76)
Stylet length (µm) 14.81 ± 0.49 (14.41–15.66) 14.46 ± 0.75 (13.49–15.26) 14.31 ± 0.28 (14.01–14.66)
Spicules (µm) 17.07± 1.35 (15.47–19.14) 19.16± 0.61 (18.25–19.65) 18.15 ± 0.58 (17.37–19)
Gubernaculum (µm) 4.92± 0.34 (4.46–5.31) 5.26± 0.27 (4.95–5.66) 5.07 ± 0.43 (4.56–5.72)
V or T 35.38± 2.75 (32–38.17) 37.56± 2.55 (33.72–40.6) 36.42 ± 2.76 (34.05–40.54)
a 28.85 ± 1.45 (26.5–30.3) 29.37 ± 2.33 (25.87–32.15) 27.01 ± 1.37 (25.36–28.91)
b 8.24 ± 1.21 (7.23–10.22) 9.11 ± 0.38 (8.66–9.67) 9.2 ± 1.01 (8.14–10.5)
b′ 5.31 ± 0.1 (5.2–5.43) 4.29 ± 0.45 (3.77–4.95) 4.85 ± 0.53 (4.47–5.75)
c 19.89 ± 1.48 (17.86–21.62) 20.95 ± 1.46 (18.7–22.47) 20.16 ± 1.83 (18.85–23.37)
c′ 2.29 ± 0.18 (2.09–2.51) 2.06 ± 0.08 (2–2.21) 2.26 ± 0.21 (1.92–2.45)
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