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First report of Pratylenchus vulnus associated with apple in Tunisia

Noura Chihani-Hammas

Noura Chihani-Hammas

Higher Agronomic Institute of Chott-MariemSousse, Tunisia
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Chihani-Hammas, Noura
, 
Lobna Hajji-Hedfi

Lobna Hajji-Hedfi

Higher Agronomic Institute of Chott-MariemSousse, Tunisia
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Hajji-Hedfi, Lobna
, 
Hajer Regaieg

Hajer Regaieg

Higher Agronomic Institute of Chott-MariemSousse, Tunisia
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Regaieg, Hajer
, 
Asma Larayedh

Asma Larayedh

Higher Agronomic Institute of Chott-MariemSousse, Tunisia
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Larayedh, Asma
, 
Ahmed Badiss

Ahmed Badiss

Agriculture and Agri-Food Canada (AAFC)Ontario, Canada
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Badiss, Ahmed
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Yu Qing

Yu Qing

Agriculture and Agri-Food Canada (AAFC)Ontario, Canada
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Qing, Yu
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Horrigue-Raouani Najet

Horrigue-Raouani Najet

Higher Agronomic Institute of Chott-MariemSousse, Tunisia
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Najet, Horrigue-Raouani
  
03 dic 2018
First report of Pratylenchus vulnus associated with apple in Tunisia's Cover Image
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Pubblicato online: 03 dic 2018
Pagine: 579 - 586
DOI: https://doi.org/10.21307/jofnem-2018-056
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© 2018 Noura Chihani-Hammas et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1

Photo micrographs of Pratylenchus vulnus specimens: females (B) and males (A), (C): lateral field at mid – body; (D): spermatheca; (E): pharyngeal region; (F) and (H): female tails; (G): vulval region; (I): male tail (Scale bars: A, B = 100 mm; C–I = 20 mm).
Photo micrographs of Pratylenchus vulnus specimens: females (B) and males (A), (C): lateral field at mid – body; (D): spermatheca; (E): pharyngeal region; (F) and (H): female tails; (G): vulval region; (I): male tail (Scale bars: A, B = 100 mm; C–I = 20 mm).

Figure 2

SEM micrograph of P. vulnus male morphology from Tunisia (A, B, C, D): anterior region, (E, F) : tail, (G) : lateral fields; sp : spicules, lig : 4 lateral lines ; anx : 3 labial annuli).
SEM micrograph of P. vulnus male morphology from Tunisia (A, B, C, D): anterior region, (E, F) : tail, (G) : lateral fields; sp : spicules, lig : 4 lateral lines ; anx : 3 labial annuli).

Figure 3

SEM micrograph of P. vulnus female morphology from Tunisia (A; B; C): anterior region, (D): lateral lines at vulva region, (E): vulva, (F, G): tail, (H): lateral fields).
SEM micrograph of P. vulnus female morphology from Tunisia (A; B; C): anterior region, (D): lateral lines at vulva region, (E): vulva, (F, G): tail, (H): lateral fields).

Figure 4

Phylogenetic relationships between Pratylenchus vulnus identified in Tunisia and other Pratylenchus already deposited in GenBank. The Pratylenchus sequences aligned in MEGA with CLUSTALW and the phylogenetic tree made by MEGA version 7.0 based on the Neighbor-Joining method as inferred from partial 18S rRNA gene.
Phylogenetic relationships between Pratylenchus vulnus identified in Tunisia and other Pratylenchus already deposited in GenBank. The Pratylenchus sequences aligned in MEGA with CLUSTALW and the phylogenetic tree made by MEGA version 7.0 based on the Neighbor-Joining method as inferred from partial 18S rRNA gene.

Figure 5

Phylogenetic relationships between Pratylenchus vulnus identified in Tunisia and other Pratylenchus already deposited in GenBank. The Pratylenchus sequences aligned in MEGA with CLUSTALW and the phylogenetic tree made by MEGA version 7.0 based on the Neighbor-Joining method as inferred from D2-D3 expansion segments of the 28S rRNA.
Phylogenetic relationships between Pratylenchus vulnus identified in Tunisia and other Pratylenchus already deposited in GenBank. The Pratylenchus sequences aligned in MEGA with CLUSTALW and the phylogenetic tree made by MEGA version 7.0 based on the Neighbor-Joining method as inferred from D2-D3 expansion segments of the 28S rRNA.

Morphometrics of Pratylenchus vulnus from apples stoolbeds, nurseries (males and females), and orchards from four Tunisian regions (Kasserin, Kairouan, Monastir, and Zaghouan, Tunisia)_

Stoolbed
Locality MM106 Chiha
Characters Females Males
n 5 5
L 444.4 ± 29.36 (415.71–485.86) 419.83 ± 32.43 (395.01–475.2)
Stylet length (µm) 14.29± 0.16 (14.17–14.55) 14.85 ± 0.21 (14.56–15.1)
Spicules (µm) 16.88± 0.26 (16.58–17.2)
Gubernaculum (µm) 4.8± 0.39 (4.2–5.25)
V or T 81.22± 0.73 (80.55–82.39) 34.02± 1.17 (32.11–35.2)
a 26.73 ± 1.32 (24.59–28.1) 28.99 ± 2.76 (25.2–31.4)
b 7.62 ± 0.55 (6.87–8.16) 6.72 ± 0.66 (5.88–7.5)
b′ 4.92 ± 0.9 (3.76–5.66) 4.85 ± 0.27 (4.55–5.22)
c 19.11 ± 0.44 (18.57–19.5) 20.75 ± 0.55 (19.88–21.3)
c′ 2.29 ± 0.22 (2.01–2.58) 2.14 ± 0.1 (2.01–2.28)
Nursery females
Locality Lorca Chiha Meski SMCCSPS
Characters Females Females
n 5 5
L 556.71 ± 33.21 (521.32–607.49) 512.48 ± 51.46 (450.11–578.12)
Stylet length (µm) 15. 45± 0.31 (15.17–15.93) 14. 44± 0.6 (13.69–15.19)
Spicules (µm)
Gubernaculum (µm)
V or T 78.77± 1.31 (76.95–80.55) 79.14 ± 1.74 (76.58–81.3)
a 28.8 ± 4.12 (25.93–35.73) 28.88 ± 3.72 (25.72–35.33)
b 7.82 ± 1.33 (6.1–9.56) 7.97 ± 0.84 (7.12–9.18)
b′ 5.26 ± 0.11 (5.16–5.41) 4.27 ± 0.27 (3.88–4.55)
c 20.22 ± 1.91 (18.17–22.7) 21.79 ± 2.81 (18.68–25.5)
c′ 2.44 ± 0.27 (2.08–2.78) 2.33 ± 0.33 (2.04–2.91)
Nursery males
Locality Lorca Chiha Anna Manzel Nour Meski SMCCSPS
Characters Males Males Males
n 5 5 5
L 483.75 ± 10.58 (468.63–492.7) 478.62 ± 30.09 (431.65–503.93) 485.52 ± 34.38(451.12–540.76)
Stylet length (µm) 14.81 ± 0.49 (14.41–15.66) 14.46 ± 0.75 (13.49–15.26) 14.31 ± 0.28 (14.01–14.66)
Spicules (µm) 17.07± 1.35 (15.47–19.14) 19.16± 0.61 (18.25–19.65) 18.15 ± 0.58 (17.37–19)
Gubernaculum (µm) 4.92± 0.34 (4.46–5.31) 5.26± 0.27 (4.95–5.66) 5.07 ± 0.43 (4.56–5.72)
V or T 35.38± 2.75 (32–38.17) 37.56± 2.55 (33.72–40.6) 36.42 ± 2.76 (34.05–40.54)
a 28.85 ± 1.45 (26.5–30.3) 29.37 ± 2.33 (25.87–32.15) 27.01 ± 1.37 (25.36–28.91)
b 8.24 ± 1.21 (7.23–10.22) 9.11 ± 0.38 (8.66–9.67) 9.2 ± 1.01 (8.14–10.5)
b′ 5.31 ± 0.1 (5.2–5.43) 4.29 ± 0.45 (3.77–4.95) 4.85 ± 0.53 (4.47–5.75)
c 19.89 ± 1.48 (17.86–21.62) 20.95 ± 1.46 (18.7–22.47) 20.16 ± 1.83 (18.85–23.37)
c′ 2.29 ± 0.18 (2.09–2.51) 2.06 ± 0.08 (2–2.21) 2.26 ± 0.21 (1.92–2.45)
Orchads
Locality Lorca Chiha Anna Manzel Nour Meski SMCCSPS
Characters Males Males Males
n 5 5 5
L 483.75 ± 10.58 (468.63–492.7) 478.62 ± 30.09 (431.65–503.93) 485.52 ± 34.38(451.12–540.76)
Stylet length (µm) 14.81 ± 0.49 (14.41–15.66) 14.46 ± 0.75 (13.49–15.26) 14.31 ± 0.28 (14.01–14.66)
Spicules (µm) 17.07± 1.35 (15.47–19.14) 19.16± 0.61 (18.25–19.65) 18.15 ± 0.58 (17.37–19)
Gubernaculum (µm) 4.92± 0.34 (4.46–5.31) 5.26± 0.27 (4.95–5.66) 5.07 ± 0.43 (4.56–5.72)
V or T 35.38± 2.75 (32–38.17) 37.56± 2.55 (33.72–40.6) 36.42 ± 2.76 (34.05–40.54)
a 28.85 ± 1.45 (26.5–30.3) 29.37 ± 2.33 (25.87–32.15) 27.01 ± 1.37 (25.36–28.91)
b 8.24 ± 1.21 (7.23–10.22) 9.11 ± 0.38 (8.66–9.67) 9.2 ± 1.01 (8.14–10.5)
b′ 5.31 ± 0.1 (5.2–5.43) 4.29 ± 0.45 (3.77–4.95) 4.85 ± 0.53 (4.47–5.75)
c 19.89 ± 1.48 (17.86–21.62) 20.95 ± 1.46 (18.7–22.47) 20.16 ± 1.83 (18.85–23.37)
c′ 2.29 ± 0.18 (2.09–2.51) 2.06 ± 0.08 (2–2.21) 2.26 ± 0.21 (1.92–2.45)
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