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First report of Bursaphelenchus antoniae from Pinus strobus in the U.S.

Lynn K. Carta
Lynn K. Carta
 and 
R. L. Wick
R. L. Wick
   | Dec 03, 2018
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Published Online: Dec 03, 2018
Page range: 473 - 478
DOI: https://doi.org/10.21307/jofnem-2018-052
Keywords
© 2018 Lynn K. Carta et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1

A. Female body, B. Male body, C. Female Tail, D. Male Tail.
A. Female body, B. Male body, C. Female Tail, D. Male Tail.

Figure 2

18S, MrBayes tree with posterior probabilities on branches of Bursaphelenchus antoniae and close relatives within the ‘B. hylobianum species group’ (in Clade I of Kanzaki et al., 2015) based on a Clustal W alignment implemented in Geneious ver. 7.1.7 (Biomatters, Auckland, NZ) using the MRBAYES plugin with Chain Length 1,100,000, Burnin 110,000, mean -LnL - 7438.56.
18S, MrBayes tree with posterior probabilities on branches of Bursaphelenchus antoniae and close relatives within the ‘B. hylobianum species group’ (in Clade I of Kanzaki et al., 2015) based on a Clustal W alignment implemented in Geneious ver. 7.1.7 (Biomatters, Auckland, NZ) using the MRBAYES plugin with Chain Length 1,100,000, Burnin 110,000, mean -LnL - 7438.56.

Figure 3

28S MrBayes tree with posterior probabilities on branches of B. antoniae based on a Clustal W alignment implemented in Geneious ver. 7.1.7 (Biomatters, Auckland, NZ) with Chain Length 1,100,000, Burnin 110,000, mean -LnL 3407.0.
28S MrBayes tree with posterior probabilities on branches of B. antoniae based on a Clustal W alignment implemented in Geneious ver. 7.1.7 (Biomatters, Auckland, NZ) with Chain Length 1,100,000, Burnin 110,000, mean -LnL 3407.0.

Primers used for PCR and sequencing.

Primers Direction Sequence (5'-3') Loci PCR Sequencing Reference
18S-CL-F3 F CTTGTCTCAAAGATTAAGCCATGCAT 18S √ √ This study
1912R R TTTACGGTCAGAACTAGGG 18S √ √ Holterman et al. (2006)
18S-530R R GCGGCTGCTGGCACCACACTT 18S √ Thomas (2011)
530F F AAGTCTGGTGCCAGCAGCCGC 18S √ Thomas (2011)
D2A F ACAAGTACCGTGAGGGAAAGTTG 28S √ √ Nunn (1992)
D3B R TCGGAAGGAACCAGCTACTA 28S √ √ Nunn (1992)
D3A F GACCCGTCTTGAAACACGGA 28S √ Nunn (1992)
ITS-CL-F2 F ATTACGTCCCTGCCCTTTGTA ITS √ √ This study
VRAIN 2R R TTTCACTCGCCGTTACTAAGGGAATC ITS √ √ Vrain et al. (1992)
rDNA1.58S R ACGAGCCGAGTGATCCACCG ITS √ Cherry et al. (1997)
COI-CL-F8 F AGAGAGTTCTAATCATAAAGATATTGG COI √ √ This study
COI-R2 R GTAGCAGCAGTAAAATAAGCACG COI √ √ Kanzaki and Futai (2002)
COI-F2 F CCTGTCTTGGCTGGTGCTATTAC COI √ Kanzaki and Futai (2002)

Summary of 28S rDNA sequences in Figure 3 tree

Taxon Isolate/Strain Accession Length (bp) Locality
Bursaphelenchus abietinus 137 AY508074 724 Austria
Bursaphelenchus antoniae 104F25F3 MA, USA
Bursaphelenchus antoniae AM279710 Portugal
Bursaphelenchus chengi HLi104111UGMD EU107359 741 Taiwan
Bursaphelenchus hellenicus 154 AY508083 724 Greece
Bursaphelenchus hofmanni 155 AY508084 725 Germany
Bursaphelenchus hylobianum Ne-2-98 KT806477 782 China
Bursaphelenchus niphades BnFFPRI AB849479 708 Japan
Bursaphelenchus parantoniae JH2015 KT223042 786 Belgium
Bursaphelenchus rainulfi BrBRA KF978102 785 Brazil
Bursaphelenchus rufipennis AB368530 1,241 Alaska, USA

Summary of 18S rDNA sequences in Figure 2 tree.

Taxon Isolate/Strain Accession Length (bp) Locality
Bursaphelenchus n.sp. 104F33 978 MA, USA
Bursaphelenchus abietinus 137 AY508011 1,706 Austria
Bursaphelenchus antoniae – AM279709 1,650 Portugal
Bursaphelenchus borealis 138 AY508012 1,698 Germany
Bursaphelenchus chengi – KT599480 1,748 Taiwan
Bursaphelenchus crenati PL-21 KU683736 1,676 Poland
Bursaphelenchus gerberae 169 AY508024 1,653 Trinidad & Tobago
Bursaphelenchus hellenicus 154 AY508017 1,706 Greece
Bursaphelenchus hylobianum 160 AY508019 1,709 China
Bursaphelenchus niphades NK203 AB849465 1,564 Japan
Bursaphelenchus parantoniae JH-2015 KT223041 1,748 Belgium
Bursaphelenchus paraparvispicularis 38717 GQ421483 1,642 Hong Kong, China
Bursaphelenchus parapinasteri Zhoushan KT878515 1,648 China
Bursaphelenchus rainulfi Ne27/04 AM397017 1,687 Brazil
Bursaphelenchus rufipennis – AM397017 1,699 Alaska, USA
Bursaphelenchus sakishimanus – LC027461 1,699 Ishigaki Is., JP
Bursaphelenchus sinensis – AB232162 2,525 Japan
eISSN:
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Language:
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