<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Juvenile, female and male nematodes were discovered in wood chips of white pine <italic>Pinus strobus</italic> from Ashley Falls, MA. Initial observations suggested these nematodes might be PWN, but closer morphological and molecular characterization proved otherwise. Comparison of measured features with those in the literature indicated this nematode population had some unique characteristics. The specimens were identified as <italic>Bursaphelenchus antoniae</italic> Penas et al., 2006 based on 18S rDNA molecular sequence vs only 95% similarity with PWN <italic>B. xylophilus</italic>. Compared to the previously described Portuguese population of <italic>B. antoniae</italic>, the sequences generated for the MA population were 98.3% similar in the ITS1, 2 rDNA and 99.9% similar for 28S rDNA. There was 99.2% similarity between the COI sequences of the US and Portuguese isolates of <italic>B. antoniae</italic>. This population has morphology consistent with that of Penas et al., 2006; however, the female tail on this MA pine population is mucronate and more attenuated than in <italic>B. antoniae</italic> from Portuguese <italic>P. pinaster</italic> found in association with <italic>Hylobius</italic> sp. Ecological associations of both populations of <italic>B. antoniae</italic> are discussed.</p>
</abstract>

Juvenile, female and male nematodes were discovered in wood chips of white pine Pinus strobus from Ashley Falls, MA. Initial observations suggested these nematodes might be PWN, but closer morphological and molecular characterization proved otherwise. Comparison of measured features with those in the literature indicated this nematode population had some unique characteristics. The specimens were identified as Bursaphelenchus antoniae Penas et al., 2006 based on 18S rDNA molecular sequence vs only 95% similarity with PWN B. xylophilus. Compared to the previously described Portuguese population of B. antoniae, the sequences generated for the MA population were 98.3% similar in the ITS1, 2 rDNA and 99.9% similar for 28S rDNA. There was 99.2% similarity between the COI sequences of the US and Portuguese isolates of B. antoniae. This population has morphology consistent with that of Penas et al., 2006; however, the female tail on this MA pine population is mucronate and more attenuated than in B. antoniae from Portuguese P. pinaster found in association with Hylobius sp. Ecological associations of both populations of B. antoniae are discussed.

First report of <italic>Bursaphelenchus antoniae</italic> from <italic>Pinus strobus</italic> in the U.S.

Journal of Nematology

This work is licensed under the Creative Commons Attribution 4.0 International License.

running-head

Juvenile, female and male nematodes were discovered in wood chips of white pine Pinus strobus from Ashley Falls, MA. Initial observations suggested these...

First report of Bursaphelenchus antoniae from Pinus strobus in the U.S.

Published Online: Dec 03, 2018

Page range: 473 - 478

DOI: https://doi.org/10.21307/jofnem-2018-052

Keywords
DNA extraction, Nematode taxonomy, Molecular identification

© 2018 Lynn K. Carta et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

First report of Bursaphelenchus antoniae from Pinus strobus in the U.S.

Published Online: Dec 03, 2018

Page range: 473 - 478

DOI: https://doi.org/10.21307/jofnem-2018-052

KeywordsDNA extraction, Nematode taxonomy, Molecular identification

© 2018 Lynn K. Carta et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
DNA extraction, Nematode taxonomy, Molecular identification