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Characterization of Dinophysis acuminata from the Yellow Sea, China, and its response to different temperatures and Mesodinium prey

Han Gao

Han Gao

  • Ocean College, Zhejiang UniversityZhoushan, China
  • Ocean College, Agricultural University of HebeiQinhuangdao, China
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Gao, Han
, 
Xinlong An

Xinlong An

Ocean College, Agricultural University of HebeiQinhuangdao, China
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An, Xinlong
, 
Lei Liu

Lei Liu

College of Life Science and Technology, Jinan UniversityGuangzhou, China
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Liu, Lei
, 
Ke Zhang

Ke Zhang

College of Life Science, Zhejiang UniversityHangzhou, China
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Zhang, Ke
, 
Daoqiong Zheng

Daoqiong Zheng

Ocean College, Zhejiang UniversityZhoushan, China
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Zheng, Daoqiong
 and 
Mengmeng Tong

Mengmeng Tong

Ocean College, Zhejiang UniversityZhoushan, China
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Tong, Mengmeng
  
Dec 12, 2017
Characterization of Dinophysis acuminata from the Yellow Sea, China, and its response to different temperatures and Mesodinium prey's Cover Image
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Article Category: Original research paper
Published Online: Dec 12, 2017
Page range: 439 - 450
Received: Mar 07, 2017
Accepted: May 23, 2017
DOI: https://doi.org/10.1515/ohs-2017-0043
Keywords
© 2017 Faculty of Oceanography and Geography, University of Gdańsk, Poland. All rights reserved
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1

Light micrographs of cultured cells: (a) Dinophysis acuminata DAYS01, from Xiaoping Island, the Yellow Sea, China; (b)Mesodinium rubrum JAMR, from Japan; (c) Mesodinium rubrum AND-A0711, from Southern Spain. Scale bars = 10 μm
Light micrographs of cultured cells: (a) Dinophysis acuminata DAYS01, from Xiaoping Island, the Yellow Sea, China; (b)Mesodinium rubrum JAMR, from Japan; (c) Mesodinium rubrum AND-A0711, from Southern Spain. Scale bars = 10 μm

Fig. 2

Neighbor-joining phylogenetic tree of dinoflagellates inferred from mitochondrial cox1. The corresponding GenBank accession number follows the name of each organism. Numbers at nodes are interior branch test values for 1000 replicates. The scale bar represents the number of substitutions per site.
Neighbor-joining phylogenetic tree of dinoflagellates inferred from mitochondrial cox1. The corresponding GenBank accession number follows the name of each organism. Numbers at nodes are interior branch test values for 1000 replicates. The scale bar represents the number of substitutions per site.

Fig. 3

(a, b) Growth responses of D. acuminata (solid lines) under multiple temperature (10, 15 and 20°C) and two strains of ciliates (a: JAMR b: AND-A0711, dash lines); (c, d) Toxin profiles of D. acuminata (DAYS01) fed with M. rubrum from Japan (JAMR) and Spain (AND-A0711) under multiple temperatures (10, 15 and 20°C). Error bars: standard error
(a, b) Growth responses of D. acuminata (solid lines) under multiple temperature (10, 15 and 20°C) and two strains of ciliates (a: JAMR b: AND-A0711, dash lines); (c, d) Toxin profiles of D. acuminata (DAYS01) fed with M. rubrum from Japan (JAMR) and Spain (AND-A0711) under multiple temperatures (10, 15 and 20°C). Error bars: standard error

Fig. 4

LC-MS/MS chromatograms of OA, DTX1 (a) and PTX2 (b) in Dinophysis acuminata (DAYS01) cultures under 10, 15 and 20°C
LC-MS/MS chromatograms of OA, DTX1 (a) and PTX2 (b) in Dinophysis acuminata (DAYS01) cultures under 10, 15 and 20°C

Growth rates and maximum yield in cultures of Dinophysis acuminata isolated from the Yellow Sea, China, at three temperatures, when fed on ciliates Mesodinium rubrum from Japan (JAMR) and Spain (AND-A0711) (mean ± standard error, n = 2)

Temp. (°C) JAMR AND-A0711
Growth rate (d-1) Max yield (cells ml-1) Growth rate (d-1) Max yield (cells ml-1)
10 0.09 ± 0.03 (Day 1-Day9)

Time indicates the exponential growth period of each treatment

 112 ± 27 0.09 ± 0.01 (Day 1-Day9) 120 ± 3
15 0.21 ± 0.015 (Day 1-Day18) 0.10 ± 0.001 (Day 1-Day21) 2483 ± 46 360 ± 52
20 0.29 ± 0.008 (Day 1-Day18) 0.22 ± 0.004 (Day 1-Day15) 7150 ± 270 1343 ± 123
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