<abstract xmlns="http://www.w3.org/1999/xhtml">

<p><italic>Dinophysis</italic> species are distributed worldwide and cause diarrheic shellfish poisoning (DSP). This paper documents the first successful culture of a <italic>Dinophysis</italic> (DAYS01) strain taken off Xiaoping Island, the Yellow Sea, China. The strain was identified as <italic>Dinophysis acuminata</italic> Claparède &amp; Lachmann by morphological and phylogenetic analysis. The effects of temperature and different <italic>Mesodinium</italic> prey on the physiological and toxigenic characteristics of <italic>D. acuminata</italic> DAYS01 were also investigated. The results showed that the toxin concentration (toxin amount per ml of culture) increased with an increase in cell densities. Okadaic acid (OA), dinophysistoxin 1 (DTX1) and pectenotoxin 2 (PTX2) were the major toxin components of DAYS01, with the highest recorded toxin content of 5.66, 0.56 and 192.87 ng ml<sup>-1</sup> culture medium, respectively. The prey type significantly influenced the growth yield in <italic>Dinophysis</italic> cultures. There was an effect of prey on the total toxin content, suggesting that the origin or strain of ciliate directly impacted the <italic>D. acuminata</italic> blooms and the overall toxin concentration in the system</p>
</abstract>

Dinophysis species are distributed worldwide and cause diarrheic shellfish poisoning (DSP). This paper documents the first successful culture of a Dinophysis (DAYS01) strain taken off Xiaoping Island, the Yellow Sea, China. The strain was identified as Dinophysis acuminata Claparède & Lachmann by morphological and phylogenetic analysis. The effects of temperature and different Mesodinium prey on the physiological and toxigenic characteristics of D. acuminata DAYS01 were also investigated. The results showed that the toxin concentration (toxin amount per ml of culture) increased with an increase in cell densities. Okadaic acid (OA), dinophysistoxin 1 (DTX1) and pectenotoxin 2 (PTX2) were the major toxin components of DAYS01, with the highest recorded toxin content of 5.66, 0.56 and 192.87 ng ml-1 culture medium, respectively. The prey type significantly influenced the growth yield in Dinophysis cultures. There was an effect of prey on the total toxin content, suggesting that the origin or strain of ciliate directly impacted the D. acuminata blooms and the overall toxin concentration in the system

Characterization of <italic>Dinophysis acuminata</italic> from the Yellow Sea, China, and its response to different temperatures and <italic>Mesodinium</italic> prey

Oceanological and Hydrobiological Studies

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Characterization of  from the Yellow Sea, China, and its response to different temperatures and  prey

Dinophysis species are distributed worldwide and cause diarrheic shellfish poisoning (DSP). This paper documents the first successful culture of a Dinophysis...

Temp. (°C)	JAMR		AND-A0711
Temp. (°C)	Growth rate (d^-1)	Max yield (cells ml^-1)	Growth rate (d^-1)	Max yield (cells ml^-1)
10	0.09 ± 0.03 (Day 1-Day9) Time indicates the exponential growth period of each treatment	112 ± 27	0.09 ± 0.01 (Day 1-Day9)	120 ± 3
15	0.21 ± 0.015 (Day 1-Day18)	0.10 ± 0.001 (Day 1-Day21)	2483 ± 46	360 ± 52
20	0.29 ± 0.008 (Day 1-Day18)	0.22 ± 0.004 (Day 1-Day15)	7150 ± 270	1343 ± 123

Characterization of Dinophysis acuminata from the Yellow Sea, China, and its response to different temperatures and Mesodinium prey

Article Category: Original research paper

Publié en ligne: 12 déc. 2017

Pages: 439 - 450

Reçu: 07 mars 2017

Accepté: 23 mai 2017

DOI: https://doi.org/10.1515/ohs-2017-0043

© 2017 Faculty of Oceanography and Geography, University of Gdańsk, Poland. All rights reserved

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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Growth rates and maximum yield in cultures of Dinophysis acuminata isolated from the Yellow Sea, China, at three temperatures, when fed on ciliates Mesodinium rubrum from Japan (JAMR) and Spain (AND-A0711) (mean ± standard error, n = 2)