1. bookVolume 40 (2017): Issue 1 (March 2017)
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Development of Simple Multiplex Real-Time PCR Assays for Foodborne Pathogens Detection and Identification On Lightcycler

Published Online: 15 Mar 2017
Page range: 53 - 58
Received: 18 Nov 2016
Accepted: 22 Dec 2016
Journal Details
License
Format
Journal
First Published
08 Sep 2014
Publication timeframe
2 times per year
Languages
English
Copyright
© 2020 Sciendo

Most acute intestinal diseases are caused by food-borne pathogens. A fast and simple real-time PCR-based procedure for simultaneous detection of food contamination by any of the five food-borne pathogens: Campylobacter jejuni, Mycobacterium bovis, Enterobacter sakazaki, Shigella boydii, Clostridium perfrigens using multiplex EvaGreen real-time PCR for LightCycler was developed and evaluated. Real-time qPCR showed excellent sensitivity. Tm calling and Melting Curve Genotyping (MCG) were used for analysis of PCR product melting curves. The Melting Curve Genotyping option showed good performance for discrimination of positive samples containing DNA of single pathogen or pathogen mixtures from negative samples.

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