1. bookVolume 51 (2017): Issue 2 (April 2017)
Journal Details
License
Format
Journal
eISSN
1336-0329
First Published
30 Mar 2016
Publication timeframe
4 times per year
Languages
English
access type Open Access

Adverse eff ects of polymeric nanoparticle poly(ethylene glycol)- block-polylactide methyl ether (PEG-b-PLA) on steroid hormone secretion by porcine granulosa cells

Published Online: 13 Jun 2017
Volume & Issue: Volume 51 (2017) - Issue 2 (April 2017)
Page range: 96 - 104
Journal Details
License
Format
Journal
eISSN
1336-0329
First Published
30 Mar 2016
Publication timeframe
4 times per year
Languages
English
Abstract

Objectives. Development of nanoparticles (NPs) for biomedical applications, including medical imaging and drug delivery, is currently undergoing a dramatic expansion. Diverse effects of different type NPs relating to mammalian reproductive tissues have been demonstrated. Th e objective of this study was to explore the in vitro effects of polymeric nanoparticle poly(ethylene glycol)-blockpolylactide methyl ether (PEG-b-PLA NPs) on functional state and viability of ovarian granulosa cells (GCs), which play an important role in maintaining ovarian function and female fertility.

Methods. The GCs isolated from porcine ovarian follicles were incubated with the different concentrations of PEG-b-PLA NPs (PEG average Mn=350 g/mol and PLA average Mn=1000 g/mol; 0.2-100 μg/ml) or poly(ethylene glycol) with an average molecular weight of 300 (PEG-300; 0.2- 40 mg/ml) in the presence or absence of stimulators, follicle-stimulating hormone (FSH; 1 μg/ml), androstenedione (100 nM), forskolin (10 μM) or 8Br-cAMP (100 μM), for different time periods (24, 48, 72 h). At the end of the incubation, progesterone and estradiol levels produced by GCs were measured in the culture media by radioimmunoassay. Th e viability of GCs was determined by the method using a colorimetric assay with MTT.

Results. Treatment of GCs with PEG-b-PLA NPs induced a significant decrease in basal as well as FSH-stimulated progesterone secretion above the concentration of 20 and 4 μg/ml, respectively. Moreover, PEG-b-PLA NPs reduced forskolin-stimulated, but not cAMP-stimulated progesterone production by GCs. A dose-dependent inhibition of androstenedione-stimulated estradiol release by GCs was found by the action of PEG-b-PLA NPs. Incubation of GCs with PEG-300 significantly inhibited basal as well as FSH-stimulated progesterone secretion above the concentration of 40 mg/ml. PEG-b-PLA NPs and PEG-300 significantly reduced the viability of GCs at the highest tested concentrations (100 μg/ml and 40 mg/ml, respectively).

Conclusions. The obtained results indicate that polymeric NPs PEG-b-PLA might induce alterations in steroid hormone production by ovarian GCs and thereby could modify reproductive functions.

Keywords

Campagnolo L, Massimiani M, Magrini A, Camaioni A, Pietroiusti A. Physico-chemical properties mediating reproductive and developmental toxicity of engineered nanomaterials. Curr Med Chem 19, 4488-4494, 2012.10.2174/092986712803251566Search in Google Scholar

De Jong WH, Borm PJ. Drug delivery and nanoparticles: applications and hazards. Int J Nanomedicine 3, 133-149, 2008.10.2147/IJN.S596Search in Google Scholar

Du JZ, Tang LY, Song WJ, Shi Y, Wang J. Evaluation of polymeric micelles from brush polymer with poly(ε- caprolactone)-b-poly(ethyleneglycol) side chains as drug carrier. Biomacromolecules 10, 2169-2174, 2009.10.1021/bm900345mSearch in Google Scholar

El-Ansary A, Al-Daihan S. On the toxicity of therapeutically used nanoparticles: an overview. J Toxicol 2009, 754810, 2009.10.1155/2009/754810Search in Google Scholar

Ema M, Kobayashi N, Naya M, Hanai S, Nakanishi J. Reproductive and developmental toxicity studies of manufactured nanomaterials. Reprod Tox 30, 343-352, 2010.10.1016/j.reprotox.2010.06.002Search in Google Scholar

Ferrandina G, Corrado G, Licameli A, Lorusso D, Fuoco G, Pisconti S, Scambia G. Pegylated liposomal doxorubicin in the management of ovarian cancer. Th er Clin Risk Manag 6, 463-483, 2010.10.2147/TCRM.S3348Search in Google Scholar

Gajdova M, Jakubovsky J, Valky J. Delayed eff ects of neonatal exposure to Tween 80 on female reproductive organs in rats. Food Chem Toxicol 31,183-190, 1993.10.1016/0278-6915(93)90092-DSearch in Google Scholar

Iavicoli I, Fontana L, Leso V, Bergamaschi A. Th e eff ects of nanomaterials as endocrine disruptors. Int J Mol Sci 14, 16732-16801, 2013.10.3390/ijms140816732375993523949635Search in Google Scholar

Jamnongjit M, Hammes SR. Ovarian steroids: the good, the bad, and the signals that raise them. Cell Cycle 5, 1178-1183, 2006.10.4161/cc.5.11.2803148278816760656Search in Google Scholar

Kedar U, Phutane P, Shidhaye S, Kadam V Advances in polymeric micelles for drug delivery and tumor targeting. Nanomedicine 6, 714-729, 2010.10.1016/j.nano.2010.05.00520542144Search in Google Scholar

Kolena J, Scsukova S, Jezova M, Furdova J, Tatara M, Jasem P. Eff ect of phospholipids on the reconstitution and thermal stability of delipidated rat ovarian luteinizing hormone/human chorionic gonadotropin receptors in proteoliposomes. Mol Cell Endocrinol 113, 53-60, 1995.10.1016/0303-7207(95)03610-JSearch in Google Scholar

Lu X, Kong X, Lobie PE, Chen Ch, Zhu T. Nanotoxicity: a growing need for study in the endocrine system. Small 9, 1654-1671, 2013.10.1002/smll.20120151723401134Search in Google Scholar

Moritz M, Gezske-Moritz M. Recent developments in the application of polymeric nanoparticles as drug carriers. Adv Clin Exp Med 24, 749-758, 2015.10.17219/acem/3180226768624Search in Google Scholar

Oberdoster G. Safety assessment for nanotechnology and nanomedicine: concept of nanotoxicology. J Intern Med 267, 89-105, 2009.10.1111/j.1365-2796.2009.02187.x20059646Search in Google Scholar

Patel T, Zhou J, Piepmeier JM, Saltzman WM. Polymeric nanoparticles for drug delivery to the central nervous system. Adv Drug Deliv Rev 163, 93-99, 2012.Search in Google Scholar

Pelham RW, Nix LC, Chavira RE, Cleveland MVB, Stetson P. Clinical trial: si ngle- and multiple-dose pharmacokinetics of polyethylene glycol (PEG-3350) in young and elderly subjects. Aliment Pharmacol Th er 25, 256-265, 2008.10.1111/j.1365-2036.2008.03727.x18462266Search in Google Scholar

Pottler M, Staicu A, Zaloga J, Unterweger H, Weigel B, Schreiber E, Hofmann S, Wiest I, Jeschke U, Alexiou Ch, Janko Ch. Genotoxicity of superparamagnetic iron oxide nanoparticles in granulosa cells. Int J Mol Sci 16, 26280-26290, 2015.10.3390/ijms161125960466181926540051Search in Google Scholar

Rollerova E, Scsukova S, Jurcovicova J, Mlynarcikova A, Szabova E, Kovriznych J, Zeljenkova D. Polymeric nanoparticles - targeted drug delivery systems for treatment of CNS disorders and their possible endocrine disrupting activities. Endocr Regul 45, 49-60, 2011.Search in Google Scholar

Rollerova E, Jurcovicova J, Mlynarcikova A, Sadlonova I, Bilanicova D, Wsolova L, Kiss A, Kovriznych J, Kronek J, Ciampor F, Vavra I, Scsukova S. Delayed adverse eff ects of neonatal exposure to polymeric nanoparticle poly(ethylene glycol)-block-polylactide methyl ether on hypothalamic-pituitary-ovarian axis development and function in Wistar rats. Reprod Toxicol 57, 165-175, 2015a.10.1016/j.reprotox.2015.07.07226193689Search in Google Scholar

Rollerova E, Tulinska J, Liskova A, Kuricova M, Kovriznych J, Mlynarcikova A, Kiss A, Scsukova S. Titanium dioxide nanoparticles: some aspects of toxicity/focus on the development. Endocr Regul 49, 97-112, 2015b.10.4149/endo_2015_02_9725960011Search in Google Scholar

Scsukova S, Mlynarcikova A, Kiss A, Rollerova E. Eff ect of polymeric nanoparticle poly(ethylene glycol)-blockpoly(lactic acid) (PEG-b-PLA) on in vitro luteinizing hormone release from anterior pituitary cells of infantile and adult female rats. Neuro Endocrinol Lett 36 (Suppl 1), 88-94, 2015.Search in Google Scholar

Semete B, Booysen L, Lemmer Y, Kalombo L, Katata L, Verschoor J, Swai HS. In vivo evaluation of the biodistribution and safety of PLGA nanoparticles as drug delivery systems. Nanomedicine 6, 662-671, 2010.10.1016/j.nano.2010.02.00220230912Search in Google Scholar

Taylor U, Barchanski A, Kues W, Barcikowski S, Rath D. Impact of metal nanoparticles on germ cell viability and functionality. Reprod Domest Anim 47(Suppl. 4), 359-368, 2012.10.1111/j.1439-0531.2012.02099.x22827393Search in Google Scholar

Sharma A, Madhunapantula SV, Robertson GP. Toxicological considerations when creating nanoparticle-based drugs and drug delivery systems. Expert Opin Drug Metab Toxicol 8, 47-69, 2012.10.1517/17425255.2012.637916324536622097965Search in Google Scholar

Shen S, Du XJ, Liu J, Sun R, Zhu YH, Wang J. Delivery of bortezomib with nanoparticles for basal-like triple-negative breast cancer therapy. J Control Release 208, 14-24, 2015.10.1016/j.jconrel.2014.12.04325575864Search in Google Scholar

Shin HC, Alani AWG, Rao DA, Rockich NC, Kwon GS. Multi-drug loaded polymeric micelles for simultaneous delivery of poorly soluble anticancer drugs. J Control Release 140, 293-300, 2009.10.1016/j.jconrel.2009.04.024278785719409432Search in Google Scholar

Shin HC, Cho H, Lai TC, Kozak KR, Kolesar JM, Kwon GS. Pharmacokinetic study of 3-in-1 poly(ethylene glycol)- block-poly(D,L-lactic acid) micelles carrying paclitaxel, 17-allylamino-17-demethoxygel danamycin, and rapamycin. J Control Release 163, 93-99, 2012.10.1016/j.jconrel.2012.04.024342261222549011Search in Google Scholar

Tosi G, Constantino L, Ruozi B, Forni F, Vandelli MA. Polymeric nanoparticles for the drug delivery to central nervous system. Expert Opin Drug Deliv 5, 155-174, 2008.10.1517/17425247.5.2.15518248316Search in Google Scholar

Zhang WD, Zhao Y, Zhang HF, Wang SK, Hao ZH, Liu J, Yuan YQ, Zhang PF, Yang HD, Shen W, Li L. Alteration of gene expression by zinc oxide nanoparticles or zinc sulfate in vivo and comparison with in vitro data: A harmonious case. Th eriogenology 86, 850-861, 2016.10.1016/j.theriogenology.2016.03.00627118516Search in Google Scholar

Wang J, Liu Y, Jiao F, Lao F, Li W, Gu Y, Li Y, Ge C, Zhou G, Li B, Zhao Y, Chai Z, Chen C. Time-dependent translocation and potential impairment on central nervous system by intranasally instilled TiO2 nanoparticles. Toxicology 254, 82-90, 2008.10.1016/j.tox.2008.09.01418929619Search in Google Scholar

Wang H, Rempel GL. Introduction of polymer nanoparticles for drug delivery applications. J Nanotechnol Nanomed Nanobiotechnol 2, 008, 2015.10.24966/NTMB-2044/100008Search in Google Scholar

Wang N, Wang Z, Nie S, Song L, He T, Yang S, Yang X, Yi C, Wu Q, Gong C. Biodegradable polymeric micelles coencapsulating paclitaxel and honokiol: a strategy for breast cancer therapy in vitro and in vivo. Int J Nanomedicine 12, 1499-1514, 2017.10.2147/IJN.S124843532814128260895Search in Google Scholar

Xiao RZ, Zeng ZW, Zhou GL, Wang JJ, Li FZ, Wang AM. Recent advances in PEG-PLA block copolymer nanoparticles. Int J Nanomedicine 5, 1057-1065, 2010.10.2147/IJN.S14912300020521170353Search in Google Scholar

Xiao L, Xiong X, Sun X, Zhu Y, Yang H, Chen H, Gan L, Xu H, Yang X. Role of cellular uptake in the reversal of multidrug resistance by PEG-b-PLA polymeric micelles. Biomaterials 32, 5148-5157, 2011.10.1016/j.biomaterials.2011.03.07121546083Search in Google Scholar

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