1. bookVolume 51 (2017): Issue 2 (April 2017)
Journal Details
First Published
30 Mar 2016
Publication timeframe
4 times per year
access type Open Access

Th e eff ects of Nigella Sativa extract on renal tissue oxidative damage during neonatal and juvenile growth in propylthiouracil-induced hypothyroid rats

Published Online: 13 Jun 2017
Volume & Issue: Volume 51 (2017) - Issue 2 (April 2017)
Page range: 105 - 113
Journal Details
First Published
30 Mar 2016
Publication timeframe
4 times per year

Objective. We investigated the effects of hydroalcoholic extract of Nigella sativa (NS) on renal tissue oxidative damage associated with propylthiouracil (PTU)-induced hypothyroidism during neonatal and juvenile growth in rats.

Methods. Pregnant rats were divided into five groups designated as: 1) control; 2) propylthiouracil (PTU); 3) PTU-NS100; 4) PTU-NS200, and 5) PTU-NS400. All mothers except the control group received 0.005% PTU in their drinking water during lactation. Besides PTU, mothers in groups 3-5 received 100, 200, and 400 mg/kg of NS extract. After lactation period, the off spring continued to receive the same experimental treatment for the first 8 weeks of their life. Ten male off springs of each group were randomly selected, blood samples collected, and the kidney tissues removed.

Results. The serum thyroxin concentration in PTU group was lower than control group and improved by extract. PTU increased the renal malondialdehyde (MDA), while reduced the total thiols concentrations and catalase (CAT) and superoxide dismutase (SOD) activity compared to control group. Administration of 200 and 400 mg/kg of NS extract decreased MDA level, while it increased the total thiols and 400 mg/kg increased CAT and SOD activity in renal tissues compared to PTU group. Serum creatinine and blood urea nitrogen (BUN) in PTU group was higher than in comparison with the control group. 400 mg/kg decreased creatinine, but both 200 and 400 mg/kg improved BUN concentration compared to PTU group.

Conclusion. The results of this study demonstrate that the hydroalcoholic extract of NS has a protective effect on the renal tissue oxidative damage associated with PTU-induced hypothyroidism during neonatal and juvenile growth in rats.


Aebi HE. Catalase. In B. HU (Ed.), Methods in Enzymatic Analysis (pp. 276-286), New York, Academic Press, 1993.Search in Google Scholar

Ahmad A, Hu sain A, Mujeeb M, Khan SA, Najmi AK, Siddique NA, Anwar F. A review on therapeutic potential of Nigella sativa: A miracle herb. Asian Pac J Trop Biomed 3, 337-352, 2013.10.1016/S2221-1691(13)60075-1Search in Google Scholar

Al-Gayyar M M, Hassan HM, Alyoussef A, Abbas A, Darweish MM, El-Hawwary AA. Nigella sativa oil attenuates chronic nephrotoxicity induced by oral sodium nitrite: Eff ects on tissue fi brosis and apoptosis. Redox Rep 21, 50-60, 2016.10.1179/1351000215Y.0000000035683766726221999Search in Google Scholar

Al-Hader A, Aqel M, Hasan Z. Hypoglycemic eff ects of the volatile oil of Nigella sativa seeds. Pharm Biol 31, 96-100, 1993.10.3109/13880209309082925Search in Google Scholar

Al-Trad B, Al-Batayneh K, El-Metwally S, Alhazimi A, Ginawi I, Alaraj M, Alkofahi E, Aljumaili O, Kosba A. Nigella sativa oil and thymoquinone ameliorate albuminuria and renal extracellular matrix accumulation in the experimental diabetic rats. Eur Rev Med Pharmacol Sci 20, 2680-2688, 2016.Search in Google Scholar

Ali B, Blunden G. Pharmacological and toxicological properties of Nigella sativa. Phytother Res 17, 299-305, 2003.10.1002/ptr.130912722128Search in Google Scholar

Ali BH. Th e eff ect of Nigella sativa oil on gentamicin nephrotoxicity in rats. Am J Chin Med 32, 49-55, 2004.10.1142/S0192415X0400171015154284Search in Google Scholar

Asami T, Uchiyama M. Elevated serum creatinine levels in infants with congenital hypothyroidism: refl ection of decreased renal function? Acta Paediatr 89, 1431-1434, 2000.10.1111/j.1651-2227.2000.tb02771.xSearch in Google Scholar

Baltaci AK, Mogulkoc R, Ayyildiz M, Kafali E, Koyuncuoglu T. Lipid peroxidation in kidney and testis tissues in experimental hypothyroidism: the role of zinc. Bratisl Lek Listy 115, 498-501, 2014.10.4149/BLL_2014_097Search in Google Scholar

Beheshti F, Khazaei M, Hosseini M. Neuropharmacological eff ects of Nigella sativa. Avicenna J Phytomed 6, 104-116, 2016.Search in Google Scholar

Beheshti F, Hosseini M, Shafei MN, Soukhtanloo M, Ghasemi S, Vafaee F, Zarepoor L. Th e eff ects of Nigella sativa extract on hypothyroidism-associated learning and memory impairment during neonatal and juvenile growth in rats. Nutr Neurosci 20, 49-50, 2017.10.1179/1476830514Y.000000014425087773Search in Google Scholar

Boskabady MH , Shirmohammadi B, Jandaghi P, Kiani S. Possible mechanism(s) for relaxant eff ect of aqueous and macerated extracts from Nigella sativa on tracheal chains of guinea pig. BMC Pharmacol 4, 3, 2004.10.1186/1471-2210-4-338522815070429Search in Google Scholar

Boskabady MH, Javan H, Sajady M, Rakhshandeh H. Th e possible prophylactic eff ect of Nigella sativa seed extract in asthmatic patients. Fundam Clin Pharmacol 21, 559-566, 2007.10.1111/j.1472-8206.2007.00509.x17868210Search in Google Scholar

Boskabady MH , Keyhanmanesh R, Saadatloo MA. Relaxant eff ects of diff erent fractions from Nigella sativa L. on guinea pig tracheal chains and its possible mechanism (s). Indian J Exp Biol 46, 805-810, 2008.Search in Google Scholar

Boskabady MH , Keyhanmanesh R, Khameneh S, Doostdar Y, Khakzad MR. Potential immunomodulation eff ect of the extract of Nigella sativa on ovalbumin sensitized guinea pigs. J Zhejiang Univ Sci B 12, 201-209, 2011a.10.1631/jzus.B1000163Search in Google Scholar

Boskabady MH, Keyhanmanesh R, Khamneh S, Ebrahimi MA. Th e eff ect of Nigella sativa extract on tracheal responsiveness and lung infl ammation in ovalbumin-sensitized guinea pigs. Clinics 66, 879-887, 2011b.10.1590/S1807-59322011000500027Search in Google Scholar

Boskabady MH , Vahedi N, Amery S, Khakzad MR. Th e eff ect of Nigella sativa alone, and in combination with dexamethasone, on tracheal muscle responsiveness and lung infl ammation in sulfur mustard exposed guinea pigs. Journal of ethnopharmacology 137, 1028-1034, 2011c.10.1016/j.jep.2011.07.030Search in Google Scholar

Cooper DS. A ntithyroid drugs. N Engl J Med 311, 1353-1362, 1984.10.1056/NEJM198411223112106Search in Google Scholar

Derubertis FR, Michelis MF, Bloom ME, Mintz DH, Field JB, Davis BB. Impaired water excretion in myxedema. Am J Med 51, 41-53, 1971.10.1016/0002-9343(71)90322-6Search in Google Scholar

El-Dakhakhny M, Mady N, Lembert N, Ammon HP. Th e hypoglycemic eff ect of Nigella sativa oil is mediated by extrapancreatic actions. Planta Med 68, 465-466, 2002.10.1055/s-2002-32084Search in Google Scholar

El Daly ES. Protective eff ect of cysteine and vitamin E, Crocus sativus and Nigella sativa extracts on cisplatin-induced toxicity in rats. J Pharm Belg 53, 87-93, 1998.Search in Google Scholar

El-Kadi A, Kandil O. Th e black seed (Nigella sativa) and immunity: its eff ect on human T cell subset. Fed Proc 46, 1222-1226, 1987.Search in Google Scholar

Ellman GL. T issue sulfh ydryl groups. Arch Biochem Biophys 82, 70-77, 1959.10.1016/0003-9861(59)90090-6Search in Google Scholar

Farrokhi E, Hosseini M, Beheshti F, Vafaei F, Hadjzadeh MA, Dastghaib SS. Brain tissues oxidative damage as a possible mechanism of deleterious eff ects of propylthiouracil-induced hypothyroidism on learning and memory in neonatal and juvenile growth in rats. Basic Clin Neurosci 5, 285-294, 2014.Search in Google Scholar

Fumarola A, Di Fiore A, Dainelli M, Grani G, Calvanese A. Medical treatment of hyperthyroidism: state of the art. Exp Clin Endocrinol 118, 678, 2010.10.1055/s-0030-1253420Search in Google Scholar

Gali-Muhtasib H, El-Najjar N, Schneider-Stock R. Th e medicinal potential of black seed (Nigella sativa) and its components. In M. a. A. A. Khan (Ed.), Lead Molecules from Natural Products: Discovery and New Trends[M] (pp. 133-153): Elsevier BV. 2006.10.1016/S1572-557X(05)02008-8Search in Google Scholar

Gholamnezhad Z, Keyhanmanesh R, Boskabady MH. Anti-infl ammatory, antioxidant, and immunomodulatory aspects of Nigella sativa for its preventive and bronchodilatory eff ects on obstructive respiratory diseases: A review of basic and clinical evidence. J Funct Foods 17, 910-927, 2015.10.1016/j.jff.2015.06.032Search in Google Scholar

Hadjzadeh MA , Khoei A, Hadjzadeh Z, Parizady M. Ethanolic extract of nigella sativa L seeds on ethylene glycolinduced kidney calculi in rats. Urol J 4, 86-90, 2007.Search in Google Scholar

Hammad FT, Lubbad L.Th e eff ect of thymoquinone on the renal functions following ischemia-reperfusion injury in the rat. Int J Physiol Pathophysiol Pharmacol 8, 152-159, 2016.Search in Google Scholar

Hosseini M, Hadjzadeh MA, Derakhshan M, Havakhah S, Rassouli FB, Rakhshandeh H, Saffarzadeh F. Th e benefi - cial eff ects of olibanum on memory defi cit induced by hypothyroidism in adult rats tested in Morris water maze. Arch Pharm Res 33, 463-468, 2010.10.1007/s12272-010-0317-zSearch in Google Scholar

Hosseini M, Pourganji M, Khodabandehloo F, Soukhtanloo M, Zabihi H. Protective eff ect of L-arginine against oxidative damage as a possible mechanism of its benefi cial properties on spatial learning in ovariectomized rats. Basic Clin Neurosci 3, 36-44, 2012.Search in Google Scholar

Houghton PJ, Zarka R, de las Heras B, Hoult JR. Fixed oil of Nigella sativa and derived thymoquinone inhibit eicosanoid generation in leukocytes and membrane lipid peroxidation. Planta Med 61, 33-36, 1995.10.1055/s-2006-957994Search in Google Scholar

Iweala E, Obichi I, Omotosho O. Biochemical and histological responses of hepatotoxic rats fed Musa paradisiaca L. supplemented diet. Int J Pharmacol 4, 471-477, 2011.10.3923/ijp.2011.471.477Search in Google Scholar

Javanbakht J , Hobbenaghi R, Hosseini E, Bahrami A M, Khadivar F, Fathi S, Hassan MA. Histopathological investigation of neuroprotective eff ects of Nigella sativa on motor neurons anterior horn spinal cord aft er sciatic nerve crush in rats. Pathol Biol (Paris) 61, 250-253, 2013.10.1016/j.patbio.2013.03.007Search in Google Scholar

Karimi G, Ramezani M, Tahoonian Z. Cisplatin nephrotoxicity and protection by milk thistle extract in rats. Evid Based Complement. Alternat Med 2, 383-386, 2005.10.1093/ecam/neh103Search in Google Scholar

Kanter M. Nigella sativa and derived thymoquinone prevents hippocampal neurodegeneration aft er chronic toluene exposure in rats. Neurochem Res 33, 579, 2008.10.1007/s11064-007-9481-zSearch in Google Scholar

Katyare SS, Bangur CS, Howland JL. Is respiratory activity in the brain mitochondria responsive to thyroid hormone action? A critical reevaluation. Biochem J 302, 857-860, 1994.10.1042/bj3020857Search in Google Scholar

Keyhanmanesh R, Boskabady MH, Khamneh S, Doostar Y. Eff ect of thymoquinone on the lung pathology and cytokine levels of ovalbumin-sensitized guinea pigs. Pharmacol Rep 62, 910-916, 2010.10.1016/S1734-1140(10)70351-0Search in Google Scholar

Khan N, Sharma S, Sultana S. Nigella sativa (black cumin) ameliorates potassium bromate-induced early events of carcinogenesis: diminution of oxidative stress. Hum Exp Toxicol 22, 193-203, 2003.10.1191/0960327103ht349oa12755470Search in Google Scholar

Khodabandehloo F, Hosseini M, Rajaei Z, Soukhtanloo M, Farrokhi E, Rezaeipour M. Brain tissue oxidative damage as a possible mechanism for the deleterious eff ect of a chronic high dose of estradiol on learning and memory in ovariectomized rats. Arq Neuropsiquiatr 71, 313-319, 2013.10.1590/0004-282X20130027Search in Google Scholar

Madesh M, Ba lasubramanian KA. Microtiter plate assay for superoxide dismutase using MTT reduction by superoxide. Indian J Biochem Biophys 35, 184-188, 1998.Search in Google Scholar

Mahmoud M, El ‐Abhar H, Saleh S. Th e eff ect of Nigella sativa oil against the liver damage induced by Schistosomamansoniinfection in mice. J Ethnopharmacol 79, 1-11, 2002.10.1016/S0378-8741(01)00310-5Search in Google Scholar

Martinez B, del Hoyo P, Martin MA, Arenas J, Perez-Castillo A, Santos A. Th yroid hormone regulates oxidative phosphorylation in the cerebral cortex and striatum of neonatal rats. J Neurochem 78, 1054-1063, 2001.10.1046/j.1471-4159.2001.00487.xSearch in Google Scholar

Mohebbati R, Shafei MN, Soukhtanloo M, Mohammadian Roshan N, Khajavi Rad A, Anaeigoudari A, Beheshti F. Adriamycin-induced oxidative stress is prevented by mixed hydro-alcoholic extract of Nigella sativa and Curcuma longa in rat kidney. Avicenna J Phytomed 6, 86-94, 2016.Search in Google Scholar

Moses AM, Scheinman SJ. Th e kidneys and electrolyte metabolism in hypothyroidism. In : Braveman LE, Utiger RD, eds. Werner and Ingbar’s Th e Th yroid. 7th ed. Philadelphia, PA: Lippincott-Raven Publishers; 812-815, 1996.Search in Google Scholar

Mousavi G. Stu dy on the eff ect of black cumin (Nigella sativa Linn.) on experimental renal ischemia-reperfusion njury in rats. Acta Cir Bras 30, 542-550, 2015.10.1590/S0102-865020150080000005Search in Google Scholar

Nakahama H, Nakamura H, Kitada O, Sugita M. Chronic drug-induced tubulointerstitial nephritis with renal failure associated with propylthiouracil therapy. Nephrol Dial Transplant 14, 1263-1265, 1999.10.1093/ndt/14.5.1263Search in Google Scholar

Nayak B, Burman K. Th yrotoxicosis and Th yroid Storm. Endocrinol Metab Clin North Am 35, 663-686, 2006.10.1016/j.ecl.2006.09.008Search in Google Scholar

Ponsoye M, Paule R, Gueutin V, Deray G, Izzedine H. [Kidney and thyroid dysfunction]. Nephrol Th er 9, 13-20, 2013.10.1016/j.nephro.2012.06.005Search in Google Scholar

Pourbakhsh H, Taghiabadi E, Abnous K, Hariri AT, Hosseini SM, Hosseinzadeh H. Eff ect of Nigella sativa fi xed oil on ethanol toxicity in rats. Iran J Basic Med Sci 17, 1020-1031, 2014.Search in Google Scholar

Prasad GV, Bastacky S, Johnson JP. Propylthiouracil-induced diff use proliferative lupus nephritis: review of immunological complications. J Am Soc Nephrol 8, 1205-1210, 1997.10.1681/ASN.V871205Search in Google Scholar

Qidwai W, Hamza HB, Qureshi R, Gilani A. Eff ectiveness, safety, and tolerability of powdered Nigella sativa (kalonji) seed in capsules on serum lipid levels, blood sugar, blood pressure, and body weight in adults: results of a randomized, double-blind controlled trial. J Altern Complement Med 15, 639-644, 2009.10.1089/acm.2008.0367Search in Google Scholar

Reinhart SC, Moses AM, Cleary L, Scheinman SJ. Acute interstitial nephritis with renal failure associated with propylthiouracil therapy. Am J Kidney Dis 24, 575-577, 1994.10.1016/S0272-6386(12)80214-9Search in Google Scholar

Salem ML. Immu nomodulatory and therapeutic properties of the Nigella sativa L. seed. Int Immunopharmacol 5, 1749-1770, 2005.10.1016/j.intimp.2005.06.00816275613Search in Google Scholar

Samarghandian S, Azimi-Nezhad M, Mehrad-Majd H, Mirhafez SR. Th ymoquinone Ameliorates Acute Renal Failure in Gentamicin-Treated Adult Male Rats. Pharmacology 96, 112-117, 2015.10.1159/00043697526202209Search in Google Scholar

Sarandol E, Tas S, Dirican M, Serdar Z. Oxidative stress and serum paraoxonase activity in experimental hypothyroidism: eff ect of vitamin E supplementation. Cell Biochem Funct 23, 1-8, 2005.10.1002/cbf.111915386442Search in Google Scholar

Sener G, Kabasakal L, Atasoy B M, Erzik C, Velioglu-Ogunc A, Cetinel S, Yegen BC. Propylthiouracil-induced hypothyroidism protects ionizing radiation-induced multiple organ damage in rats. J Endocrinol 189, 257-269, 2006.10.1677/joe.1.0657416648293Search in Google Scholar

Seghatoleslam M, Alipour F, Shafieian R, Hassanzadeh Z, Edalatmanesh MA, Sadeghnia HR, Hosseini M. Th e eff ects of Nigella sativa on neural damage aft er pentylenetetrazole induced seizures in rats. J Tradit Complement Med 6, 262-268, 2015.10.1016/j.jtcme.2015.06.003493677227419091Search in Google Scholar

Singh PA, Bobby Z, Selvaraj N, Vinayagamoorthi R. An evaluation of thyroid hormone status and oxidative stress in undialyzed chronic renal failure patients. Indian J Physiol Pharmacol 50, 279, 2006.Search in Google Scholar

Stelios F, Geo rge P, Agathocles T. Th e role of iodine in the evolution of thyroid disease in Greece: from endemic goiter to thyroid autoimmunity. Horm 6, 25-35, 2007.Search in Google Scholar

Ulu R, Dogukan A, Tuzcu M, Gurel A, Muqbil I. Modulation of Nrf2/HO-1 by thymoquinone during cisplatininduced nephrotoxicity. Turk Neph Dial Transpl 22,182-187, 2013.10.5262/tndt.2013.1002.09Search in Google Scholar

Yaman I, Balikci E. Protective eff ects of Nigella sativa against gentamicin-induced nephrotoxicity in rats. Exp Toxicol Pathol 62,183-190, 2010.10.1016/j.etp.2009.03.00619398313Search in Google Scholar

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