1. bookVolume 28 (2015): Issue 3 (September 2015)
Journal Details
License
Format
Journal
eISSN
2300-6676
First Published
30 May 2014
Publication timeframe
4 times per year
Languages
English
access type Open Access

Designing primers potentially specific to Entamoeba gingivalis genes

Published Online: 26 Nov 2015
Volume & Issue: Volume 28 (2015) - Issue 3 (September 2015)
Page range: 159 - 163
Received: 01 Jul 2015
Accepted: 20 Aug 2015
Journal Details
License
Format
Journal
eISSN
2300-6676
First Published
30 May 2014
Publication timeframe
4 times per year
Languages
English
Abstract

Entamoeba gingivalis normally exists in the human oral cavity, namely in the gums, and brings about some specific diseases. However, it can also trigger some more serious illnesses. Among these are infections of the genital tract, acute osteomyelitis of the mandible and pulmonary abscess. Entamoeba gingivalis identification by light microscopy is difficult, hence polymerase chain reaction (PCR) is used. The contemporary primers for PCR are complement to 18S rRNA. This article informs the reader of the process that was involved in designing new primers for three genes which were thought to be present on the Entamoeba gingivalis genome, but their sequences were unknown. The newly obtained sequences of primers have better properties for identification purposes, compared to these which are currently used.

Keywords

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