Target-specific drug-delivery systems for the administration of pharmaceutical compounds enable the localization of drugs to diseased sites. Various types of drug-delivery systems utilize carriers, such as immunoglobulins, serum proteins, synthetic polymers, liposomes, and microspheres. The vesicular system of niosomes, with their bilayer structure assembled by nonionic surfactants, is able to enhance the bioavailability of a drug to a predetermined area for a period. The amphiphilic nature of niosomes promotes their efficiency in encapsulating lipophilic or hydrophilic drugs. Other additives, such as cholesterol, can be used to maintain the rigidity of the niosomes’ structure. This narrative review describes fundamental aspects of niosomes, including their structural components, methods of preparation, limitations, and current applications to various diseases.
- drug-delivery system
- medical applications
- methods of preparation
The concept of a drug-delivery system refers to a process of administering pharmaceutical compounds at a predetermined rate to achieve a therapeutic effect in humans or animals at a diseased site, and at the same time, reducing the concentration of the medication in surrounding tissues. Localized drug action enhances the efficacy of drug and reduces systemic toxic effects to tissues . Paul Ehrlich proposed the idea of targeted delivery directly to the diseased cell without damaging healthy cells in 1909, and this strategy has been known as the “magic bullet” . Since then, a number of drug carrier systems have emerged, including immunoglobulins, serum proteins, synthetic polymers, liposomes, microspheres, and niosomes . Among these systems, liposomes and niosomes are well-documented vesicular drug-delivery systems [3,4,5,6]. In general, a vesicular system is a drug-delivery platform that enables effective bioavailability of drugs through controlled release of therapeutic drugs for a prolonged period [7,8,9,10]. The vesicles consist of bilayer amphiphilic molecules that surround an aqueous compartment [8, 11, 12]. Niosomes are vesicles of nonionic surfactant (for example, alkyl ester and alkyl ether) and cholesterol that act as a carrier for amphiphilic and lipophilic drugs [7, 8, 13, 14]. Niosomes improve the therapeutic performance of encapsulated drug molecules by protecting the drug from harsh biological environments, resulting in their delayed clearance .
Novel drug development is both time consuming and expensive. The development of a new drug costs an estimated $120 million, and the journey from discovery, clinical testing, and development to regulatory approval takes decades [16,17,18,19]. Specific drug-delivery systems alleviate the urgency for bringing new drugs into the market by increasing drug selectivity and the therapeutic index, while lowering the effective dose. This narrative review discusses the role of niosomes as a drug-delivery system and details of their structure, preparation, properties, and applications.
Niosomes are spherical and consist of microscopic lamellar (unilamellar or multilamellar) structures (
Niosomes can be categorized into 3 groups based on their vesicle size, namely, small unilamellar vesicles (0.025–0.05 μm), multilamellar vesicles (>0.05 μm), and large unilamellar vesicles (>0.10 μm) .
Preparation of niosomes begins with the hydration of a surfactant and lipid mixture at elevated temperatures, followed by optional niosome size reduction in order to obtain a colloidal suspension . There are several well-studied standard methods for the preparation of niosomes. Ether injection, hand shaking, sonication, and microfluidization methods are a few examples [7, 27, 29]. Subsequently, the unentrapped drug is separated from the entrapped drug by centrifugation, gel filtration, or dialysis .
The first step in niosome production by ether injection is through the dissolution of surfactant in diethyl ether. The solution is then injected through a 14-gauge needle into an aqueous solution of drug maintained at 60°C. Subsequently, single-layer vesicles with diameters ranging from 50 to 1000 nm are formed because of the vaporization of ether . However, a small amount of residual ether frequently persists in the niosomal suspension .
In the hand-shaking method, also known as thin-film hydration technique, surfactant and cholesterol are dissolved in a volatile organic solvent and transferred to a rotary evaporator. After evaporation, a thin layer of solid mixture is deposited on the wall of the flask. The dried layer is then hydrated with an aqueous phase containing the drug of interest. This process may be carried out at room temperature with gentle agitation [15, 27].
Niosomes can also be produced through sonicating a mixture of surfactant, cholesterol, and aqueous phase containing the drug at 60°C for 3 min. The vesicles produced through this method are usually small and uniform in size [15, 27]. Microfluidization is another reproducible technique that achieves this size uniformity. Operationally, 2 fluidized streams move forward through a precisely defined micro-channel, and these 2 streams interact with each other at an ultrahigh velocity [20, 27, 30].
Alternative methods have since been defined for the preparation of niosomes. The multiple membrane extrusion method uses surfactant, cholesterol, and dicetyl phosphate in chloroform, and the mixture is evaporated to produce a thin film. The film is then hydrated with aqueous drug solution, and the suspension produced is extruded through polycarbonate membranes, which are placed in series for up to 8 passages [20, 27, 31].
The reverse-phase evaporation technique uses a mixture containing surfactant and cholesterol in a 1:1 ratio, in addition to ether and chloroform. An aqueous phase containing the target drug is added to the mixture followed by sonication at 4–5°C. Sonication is continued after adding a small amount of phosphate-buffered saline to the mixture. The organic solvent is removed at 40°C under a low pressure, and the remaining suspension is diluted with phosphate-buffered saline. After heating the mixture at 60°C for 10 min, the final product of niosomes is obtained [20, 27, 32]. The preparation of niosomes using the reverse-phase evaporation technique is illustrated in
Niosomes can be produced without the use of organic solvents using the “bubble” method. A “bubbling unit” consists of a round-bottomed flask with 3 necks positioned in a water bath; a water-cooled reflux condenser and thermometer are positioned in the first and second necks, respectively, while nitrogen is supplied through the third neck. Surfactant and cholesterol that are mixed at 70°C in a buffer are homogenized and “bubbled” at 70°C using the “bubbling unit” [20, 33]. The preparation of niosomes using this technique is illustrated in
In another variation of preparation of niosomes, a thin film resulting from evaporation of surfactant and cholesterol dissolved in chloroform is hydrated with 300 mM citric acid (pH 4.0). Then, the suspension is subjected to 3 successive freeze–thaw cycles. After sonication, the aqueous solution containing the drug is added to the suspension and vortexed. Disodium phosphate (1 M) is added to the mixture to increase the pH to 7.0–7.2. This mixture is later heated at 60°C for 10 min to produce niosomes. This method is known as the “transmembrane pH gradient (inside acidic) drug uptake process” [20, 27, 34]. Niosomes obtained by this method showed better entrapment efficiency and retention of drugs [35, 36].
The emulsion method, which uses an oil-in-water emulsion prepared from an organic solution of surfactant, cholesterol, and an aqueous solution of drug, is another technique for preparation of niosomes. The organic solvent is evaporated to obtain the final product [8, 27, 37]. By contrast, a mixture of lipids and surfactant is melted and injected into a heated aqueous phase containing the drug in the lipid injection method .
Surfactant-based niosomes are biodegradable, biocompatible, and nonimmunogenic . They act as a drug depot in the body, whereby they release drugs in a controlled manner through their closed bilayer structure, resulting in a sustained release of the enclosed drug to the target site . The therapeutic effects of drugs enclosed in niosomes are improved by reduced clearance and specific targeting . Because of their hydrophilic, amphiphilic, and lipophilic nature, niosomes are able to accommodate a wide variety of drugs with a wide range of solubility . Bioavailability of otherwise poorly soluble drugs may be improved, and the efficacy of topical applications would be enhanced with the use of niosomes. Furthermore, labile and sensitive drugs may be delivered with greater ease as niosomes protect the encapsulated active pharmaceutical ingredients from deleterious conditions both inside and outside of the body .
The stability of niosomes is mainly affected by the type of surfactant, properties of the encapsulated drug, temperature of hydration, detergent, membrane-spanning lipids, polymerization of surfactant monomers, and charged molecules [20, 21, 27]. It is crucial for the surfactant used for the preparation of niosomes to consist of a hydrophilic head and a hydrophobic tail. Generally, surfactants possessing hydrophobic tails with an alkyl (chain length from C12 to C18), perfluoroalkyl, or steroidal groups are suitable for the preparation of niosomes . Ester-type surfactants are less suitable because they are easily degraded by esterases in vivo, making them unstable in the body.
Comparatively, ether-type surfactants are a better choice. The size of niosomes increases proportionally with the increase in hydrophilic–lipophilic balance (HLB) of surfactants. If the HLB value falls between 4 and 8, niosome vesicle formation is considered relatively stable and optimal [8, 27, 40]. Addition of different types of additives together with the drug entrapped in niosomes is able to improve niosome stability. For instance, addition of cholesterol provides rigidity and reduces leaking in niosomes .
Hydration temperature plays an important role in the assembly of surfactants into vesicles and the shape and size formation of niosomes. Ideally, the temperature chosen should be above the temperature of the gel-to-liquid phase transition [8, 27, 42]. Encapsulated drugs usually interact with the head group of the surfactant and thus indirectly influence the charge and rigidity of the bilayer structure of the niosome. Hydrophobic drugs generally improve the stability of niosomes, while hydrophobic drugs decrease their stability. Interestingly, amphiphilic drugs have no obvious effect on the niosomes’ bilayer structure [27, 43].
Common problems related to preparation of niosomes include aggregation, fusion, and leaking, which are affected by the physiochemical properties of vesicles including their size, charge, lamellarity, elasticity, and thermodynamic phase. Preparation of proniosomes, which are a dried form of niosomes, might overcome the limitations mentioned because proniosomes hydrate immediately before use to yield an aqueous niosome dispersion . Studies have found that proniosomes carried better therapeutic efficacy for anti-inflammatory drugs (flurbiprofen and piroxicam) administrated via a transdermal route [45, 46].
Niosomes are generally nontoxic to humans . Researchers studied the toxic effect of surfactants in a topical niosome formulation on the proliferation of keratinocytes. The results show that ester was less toxic than ether because of enzymatic degradation of ester bonds. It had been proven that the physical form of niosomes (liquid crystal vs gel) did not influence their toxicity .
Niosomes possess a bilayer structure, which is similar to liposomes. However, the materials used to prepare niosomes confer better stability on them . Niosomes are prepared from uncharged single chain surfactant and cholesterols. By contrast, liposomes are prepared from neutral or charged double chain phospholipids. The concentration of cholesterol is higher in liposomes than in niosomes. As a result, the drug entrapment efficiency of liposomes is less than that of niosomes. Niosomes are cost-effective for industrial manufacture and do not require special storage conditions, which are essential while manufacturing liposomes. The cost of liposome preparation is high because of the unstable chemical ingredients (phospholipids), which undergo oxidative degradation. Liposomes therefore require special handling methods . Niosomes possess a longer shelf life than liposomes . They prolong the circulation of encapsulated drugs and increase metabolic stability in an emulsified form, whereas liposomes have limited shelf life because of rancidification of their lipid components [7, 47,48,49,50].
Drug-delivery systems using niosomes through transdermal, parenteral, and ophthalmic routes are well studied [27, 51, 52]. Niosomal delivery via transdermal routes is able to overcome the slow penetration rate of conventional transdermal approaches. The bioavailability and therapeutic efficacy of drugs such as diclofenac, flurbiprofen, and nimesulide increase by their incorporation into niosomal formulations. For ophthalmic drug delivery, chitosan-coated niosomal formulation of timolol maleate exhibits a greater effect in reducing intraocular pressure compared with marketed formulations, with less cardiovascular side effects . Because of their many desirable properties, niosomal formulations have been used in many other therapeutic applications, as discussed in the following sections.
Leishmaniasis is a parasitic disease invading the liver and spleen transmitted through the bite of a female sandfly. Therapeutic efficacy of amarogentin (an antileishmanial, secoiridoid glycoside isolated from Indian medicinal plant
Acquired immune deficiency syndrome (AIDS) is characterized by a severe damage to the immune system caused by the human immunodeficiency virus (HIV). Zidovudine (AZT), a pioneering anti-HIV compound, is approved for clinical use, either alone or in combination with other antiviral agents, for the treatment of AIDS and AIDS-related complex. However, there are some setbacks in AZT administration, including hematological toxicity, poor bioavailability, high first-pass metabolism, and short half-life . A study revealed that niosomal AZT formulation prolonged the drug half-life in rabbit serum . In another study, niosomes manufactured with Tween 80 were able to encapsulate a high amount of AZT, and addition of dicetyl phosphate enhanced drug release for a prolonged period (88.72% over 12 h). Changes in the micromolar ratios of nonionic surfactants with a constant ratio of cholesterol during the preparation of niosomes were associated with the changes in the encapsulation efficiency and controlled release of AZT .
Common drawbacks of cancer chemotherapy are the side effects and low therapeutic efficacy. Doxorubicin, a broad-spectrum anthracycline used for antitumor activity, has shown a dose-dependent irreversible cardiotoxic effect . However, niosomal delivery of this drug to mice bearing S-180 tumor showed increased life span and decreased proliferation of sarcoma. This might be the result of the high efficiency of niosomes in encapsulating the drug, causing a prolonged circulation in addition to alteration in drug metabolism [26, 57]. Another well-known anticancer drug, daunorubicin hydrochloride, exhibited an enhanced antitumor efficacy in its niosomal encapsulated form when compared to the free drug. The niosomal formulation destroyed Dalton’s ascitic lymphoma cells effectively in a short time. Bleomycin, a potent anticancer drug, was found to be accumulated in higher levels at the tumor site when it was encapsulated in niosomes containing 47.5% cholesterol as compared with its free drug form .
Methotrexate is an established toxic synthetic antineoplastic agent used in chemotherapy, either alone or combined with other medications, to treat various types of cancers. Studies have shown that intravenous administration of methotrexate encapsulated in niosomes to S-180 tumor-bearing mice resulted in a total regression of tumor, slower drug clearance, and a higher plasma level of methotrexate . Enhanced drug penetration was noted when 5-fluorouracil was formulated in bola-surfactant niosomes to treat skin cancer . Despite enhanced antitumor activity, in some instances, encapsulation of the drug in niosomal vesicles reduces the toxicity in normal cells, as demonstrated in a study of preparation of niosome containing vincristine. Common side effects of the drug, such as neurological toxicity, diarrhea, and alopecia, were alleviated, while antitumor activity was increased in a mouse model of S-180 sarcoma following intravenous administration of the niosome-encapsulated vincristine .
Tocotrienol, first reported for its anticancer activity in the early 1990s , was encapsulated in niosomes by Fu et al. using a film hydration method. The niosomal tocotrienol showed at least 2-fold enhancement of its cytotoxic effect in killing breast cancer cells, with 2.5-fold increase in the drug uptake in the cells. The antitumor effect of the formulation was also observed in female BALB/c nude mice implanted with breast cancer cells .
Curcumin is known to show multiple therapeutic applications including anticancer properties . A novel niosome system composed of Span 80, Tween 80, and poloxamer 188 was shown to possess high encapsulating efficiency (92.3%) for curcumin. When niosomal curcumin was added to ovarian cancer A2780 cells, enhanced cytotoxic and apoptotic effects were noted compared with freely dispersed curcumin. This might be the result of the efficient controlled release of curcumin from niosomes . Sharma et al. prepared niosomes with Tween 80 and cholesterol using a film hydration method. Two active molecules, curcumin and doxorubicin hydrochloride, were encapsulated into the prepared niosomes; curcumin was found accumulated in the shell, whereas doxorubicin hydrochloride accumulated in the inner aqueous core of the niosomes. Enhanced cytotoxicity toward cervical cancer cells (HeLa) was observed for the dual-drug-loaded niosomes .
Artemisinin, isolated from the Chinese herb
Tamoxifen citrate is a hormone antagonist administered to breast cancer patients with estrogen receptor-positive tumors . However, issues such as localization, efficiency, sustained delivery, and side effects of drugs are the major challenges for this type of cancer therapy. As such, Shaker et al. loaded the drug into niosomes and evaluated its cellular uptake, cytotoxicity, and efficiency in vivo. Niosomal tamoxifen showed enhanced cellular uptake and greater cytotoxicity against the MCF-7 breast cancer cell line and enhanced reduction in tumor volume in vivo .
Mitoxantrone has been used in chemotherapy for leukemia, lymphoma, breast and prostate cancers, and multiple sclerosis. However, administration of the drug is usually associated with severe systemic toxicity, particularly cardiotoxicity. Tila et al. formulated pH-sensitive, polymer-modified, and plasma-stable niosomes to carry the drug. Cytotoxicity of mitoxantrone niosomes was evaluated against human ovarian cancer (OVCAR-3), human breast cancer (MCF-7), and human umbilical vein endothelial cell lines. Mitoxantrone contained in the pH-sensitive niosomes showed higher cytotoxicity than conventional niosomes on the cancer cells but a lowered cytotoxic effect on the endothelial cell line. These findings indicate that the niosomal formulations may be useful in reducing the side effects of mitoxantrone .
Cisplatin, a widely used anticancer drug, acts by inducing apoptosis and necrosis. However, cisplatin usage is found to be associated with various unwarranted side effects such as nephro- and neurotoxicity . Furthermore, development of drug resistance toward cisplatin in patients is a major concern in cancer treatment. Niosomal cisplatin prepared by reverse-phase evaporation technique showed a 1.5-fold increase in cytotoxic effect against BT-20 breast cancer cells compared with the unencapsulated drug . Anticancer effects of the niosomal formulations mentioned are summarized in
Drugs/compounds encapsulated in niosomes and their anticancer effects
Drug/compound(s) Effects Reference(s) Doxorubicin Decreased proliferation of sarcoma cells [26, 57] Daunorubicin hydrochloride Destroyed Dalton’s ascitic lymphoma cells  Bleomycin Accumulated in higher levels at the tumor site  Methotrexate Enhanced antitumor activity against sarcoma  5-Fluorouracil Enhanced drug penetration in the treatment of skin cancer  Vincristine Enhanced antitumor activity against sarcoma  Tocotrienol Enhanced cytotoxicity toward breast cancer cells  Curcumin Enhanced cytotoxic and apoptotic effects toward ovarian cancer cells  Curcumin and doxorubicin hydrochloride Enhanced cytotoxicity toward cervical cancer cells  Artemisinin Cytotoxic toward melanoma cells  Tamoxifen citrate Greater cytotoxicity against breast cancer cell line  Mitoxantrone Greater cytotoxicity against human ovarian cancer and breast cancer cell lines  Cisplatin Enhanced cytotoxicity toward breast cancer cells 
Drugs/compounds encapsulated in niosomes and their anticancer effects
Inhalation therapy utilizing glucocorticosteroids such as beclomethasone dipropionate (BDP) in chronic obstructive pulmonary disease (COPD) is promising, but the common drawback of the medication is poor permeation through hydrophilic mucus to reach the glucocorticoid receptors of bronchial epithelial cells . Niosomes developed from polysorbate 20 containing BDP are well suited as a drug-delivery system for COPD patients through pulmonary delivery. This is because of the advantages offered by the niosomes, such as high drug encapsulation efficiency, strong mucus permeation, and sustained delivery to the target site . Thus, the use of niosomes overcame weaknesses of the drug and other conventional inhalation therapies. Niosomes may offer a better targeted-delivery system for patients with COPD as these nonionic vesicles remarkably increased the permeation rate of BDP through the mucosal membrane barrier. Clinical efficacy of an inhalation therapy is usually dependent on the aerodynamic size distribution of the aerosol and drug output from a nebulizer. Niosomal dispersion provides a better aerodynamic diameter than the commercial products’ dispersion. Under all conditions of nebulization, niosomes provide a higher efficacy, thus offering better targeting of corticosteroids in the treatment of COPD .
Niosomal encapsulation of isoniazid was found to effectively treat tuberculosis, with 62% of cellular uptake by macrophages. In addition, the niosomal formulation decreased the dose required and reduced the level of toxicity; these contributed to improved patient compliance. The additional advantages of the niosomal formulation are that it was site specific to where tuberculosis bacteria were harbored and it was able to maintain steady drug concentrations for up to 30 h . Another study showed that intravenous and intraperitoneal administration of rifampicin encapsulated in niosomes prepared using surfactants of sorbitan ester class:cholesterol (50:50 percent mole fraction ratio) could be used for the treatment of tuberculosis . Positive results were noted with rifampicin encapsulated in Span 85 (sorbitan trioleate)-based niosomes. The rifampicin was found accumulated in the lungs of mice, therefore offering possibility of improved antituberculosis therapy [8, 78].
Gentamycin sulfate used in the treatment of nosocomial pneumonia displays short half-life and has various side effects. Niosomal formulations may ensure that an efficient concentration of the drug is achieved in the lungs without inducing systemic effects. Gentamycin sulfate in niosomes prepared with polyoxyethylene sorbitan esters showed a significantly higher accumulation in lungs than the plain drug, indicating that niosomes are an enhanced drug-delivery system for this therapy .
Niosomal formulations of diclofenac sodium, nimesulide, and flurbiprofen exhibit greater anti-inflammatory activity than the free drugs . Niosomal formulations of diclofenac diethylammonium, aceclofenac, meloxicam, and lornoxicam used for topical application also show good anti-inflammatory activity because of the penetration of niosomes into the deeper layers of the skin [7, 80,81,82]. Additionally, mefenamic acid-loaded niosomes prepared by Kamboj et al.  demonstrated enhanced inhibition of inflammation in vivo.
Serratiopeptidase is controversially used in the treatment of arthritis, fibrocystic breast disease, chronic bronchitis, and carpal tunnel syndrome to alleviate pain and inflammation. Orally administered serratiopeptidase has been reported to show systemic side effects. Therefore, a topical formulation of serratiopeptidase that potentially reduces adverse effects and increase local effects has been developed by Shinde and Kanojiya using niosomes. The results showed that the newly formulated serratiopeptidase niosomal gel had anti-inflammatory activity comparable to that of diclofenac gel .
A number of compounds isolated from natural products have recently emerged as potential anti-inflammatory agents. Compounds isolated from
Resveratrol is a polyphenolic compound found in grape skin, peanuts, and the roots of
Flurbiprofen is commonly used in the treatment of ocular inflammatory diseases . However, challenges arise with the use of the ocular drugs, including limited ocular bioavailability because of nasolacrimal drainage, the corneal barrier, and unintentional absorption of the drugs to the systemic circulation . As a result, not more than 5% of applied drugs can reach intraocular tissues . Therefore, to enhance the ocular bioavailability of flurbiprofen, the drug was encapsulated in niosomes consisting of nonionic surfactant Span 60 using the thin-film hydration technique. The encapsulated flurbiprofen was found to rapidly reduce eye inflammation of New Zealand albino rabbits, with a higher ocular bioavailability than that of a flurbiprofen solution .
Itraconazole is the drug of choice for treating fungal infections caused by
Nisin is an antimicrobial agent used in food and pharmaceutical applications . However, its effectiveness is limited because of its inaccessibility to an inner membrane of bacteria. Hence, ethylenediaminetetraacetic acid (EDTA) is used to improve the efficacy of nisin . Niosomes encapsulating both nisin and EDTA were evaluated for their antibacterial activity. The encapsulated form of nisin and EDTA showed a better and longer-lasting effect in inhibiting
Gatifloxacin is widely used for ocular infections. Patients are required to apply the medicine several times a day, and this greatly inconveniences the patients. Therefore, Zubairu et al. designed and formulated a novel delivery system for gatifloxacin that could help in reducing the dosing frequency. A chitosan-coated niosomal formulation of gatifloxacin developed by researchers showed a longer retention time on the eyes, suggesting the potential use of the drug in the form of transcorneal delivery . Another group of scientists developed chitosan gel-embedded moxifloxacin niosomes, and the drug formulation was used for topical microbial infections .
Drugs/compounds encapsulated in niosomes and their antibacterial or antifungal effects
Drug/compound(s) Effects compared with unencapsulated form Reference(s) Itraconazole Greater activity against  Diallyl disulfide Greater activity against  Fluconazole Effective against corneal fungal infections  Miconazole Effective against  Nisin and ethylenediaminetetraacetic acid Better effect in inhibiting  Gallidermin Better antibacterial effects against  Propolis Enhanced antimicrobial effect  Gatifloxacin Effective against ocular infections  Moxifloxacin Used for topical microbial infections  Bismuth-ethanedithiol and tobramycin Effective against 
Drugs/compounds encapsulated in niosomes and their antibacterial or antifungal effects
Niosomal formulations have been used extensively in many other applications such as transdermal delivery. For example, 8-methoxypsoralen, a compound used in psoralen ultraviolet A therapy , had been formulated into niosomal vesicles and delivered topically to increase its local efficacy and safety . Another group of investigators has attempted to develop a niosomal gel formulation with acyclovir that has a superior topical bioavailability . Acyclovir is used in the treatment of herpes simplex, varicella zoster, and herpes zoster viral infections. Its usual route of oral administration is plagued by poor bioavailability and short elimination half-life . Usual topical delivery is limited by the poor intrinsic permeation of acyclovir into the basal epidermis. A goal of topical delivery is that the drug is transported through the stratum corneum, thereby effectively reaching the target tissue. The niosomal gel developed by Jacob et al.  demonstrated successful delivery of acyclovir through topical administration.
Niosomes could also potentially serve as gene delivery system. Niosomes prepared by using cationic lipid
Other uses of niosomes include the treatment of glaucoma using dorzolamide niosomes , specific niosomal drug-delivery systems that target the liver  and induction of angiogenesis using growth factor-loaded nano-niosomal gel formulations . Moghassemi et al.  formulated bovine serum albumin-loaded niosomes that have potential use in pharmaceutical and cosmetic applications.
Niosomes, a nonionic surfactant vesicular system, is a novel and efficient approach to drug delivery. With the incorporation of appropriate nonionic surfactant and cholesterol in the vesicular membrane, a wide range of drugs can be encapsulated into niosomes. In addition, niosomes possess enhanced stability and reduced toxic drug effects, with sustained release of the encapsulated drug. Furthermore, no special conditions are required for handling and storage of niosomes, compared with other drug-delivery systems such as liposomes. Appropriate modifications of niosomes, resulting in structures such as proniosomes, enable them to be used in special routes of administration. In summary, niosomes represent a highly effective tool for drug delivery in the therapeutic regime of numerous diseases and have the potential to provide more efficacious treatment than conventional drug-delivery platforms.
Drugs/compounds encapsulated in niosomes and their antibacterial or antifungal effects
|Drug/compound(s)||Effects compared with unencapsulated form||Reference(s)|
|Itraconazole||Greater activity against ||[|
|Diallyl disulfide||Greater activity against ||[|
|Fluconazole||Effective against corneal fungal infections||[|
|Miconazole||Effective against ||[|
|Nisin and ethylenediaminetetraacetic acid||Better effect in inhibiting ||[|
|Gallidermin||Better antibacterial effects against ||[|
|Propolis||Enhanced antimicrobial effect||[|
|Gatifloxacin||Effective against ocular infections||[|
|Moxifloxacin||Used for topical microbial infections||[|
|Bismuth-ethanedithiol and tobramycin||Effective against ||[|
Drugs/compounds encapsulated in niosomes and their anticancer effects
|Doxorubicin||Decreased proliferation of sarcoma cells||[|
|Daunorubicin hydrochloride||Destroyed Dalton’s ascitic lymphoma cells||[|
|Bleomycin||Accumulated in higher levels at the tumor site||[|
|Methotrexate||Enhanced antitumor activity against sarcoma||[|
|5-Fluorouracil||Enhanced drug penetration in the treatment of skin cancer||[|
|Vincristine||Enhanced antitumor activity against sarcoma||[|
|Tocotrienol||Enhanced cytotoxicity toward breast cancer cells||[|
|Curcumin||Enhanced cytotoxic and apoptotic effects toward ovarian cancer cells||[|
|Curcumin and doxorubicin hydrochloride||Enhanced cytotoxicity toward cervical cancer cells||[|
|Artemisinin||Cytotoxic toward melanoma cells||[|
|Tamoxifen citrate||Greater cytotoxicity against breast cancer cell line||[|
|Mitoxantrone||Greater cytotoxicity against human ovarian cancer and breast cancer cell lines||[|
|Cisplatin||Enhanced cytotoxicity toward breast cancer cells||[|