<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Investigations of bacterial communities and characterization of mineralogy of the environment in the Złoty Stok As-Au deposit were carried out. PXRD analysis revealed the presence of picropharmacolite as the most common secondary arsenic mineral in the mine. Total DNA was extracted from slime streams or slime biofilms samples to investigate the bacterial communities. PCR amplification of 16S rDNA was performed followed by subcloning of its products. Over 170 clones were analyzed by means of RFLP method. Eight group of clones representing different restriction patterns were identified. The nucleotide sequences of their inserts suggest that bacteria present in the mine environment belong to: <italic>Flavobacteria</italic>, <italic>Sphingobacteriia</italic>, <italic>Bacteroides</italic>, <italic>Proteobacteria</italic>, <italic>Mollicutes</italic> and <italic>Firmicutes</italic>. The metagenomic approach allows to demonstrate a higher diversity of microbiota than classical microbiological studies of cultivable isolates.</p>
</abstract>

Investigations of bacterial communities and characterization of mineralogy of the environment in the Złoty Stok As-Au deposit were carried out. PXRD analysis revealed the presence of picropharmacolite as the most common secondary arsenic mineral in the mine. Total DNA was extracted from slime streams or slime biofilms samples to investigate the bacterial communities. PCR amplification of 16S rDNA was performed followed by subcloning of its products. Over 170 clones were analyzed by means of RFLP method. Eight group of clones representing different restriction patterns were identified. The nucleotide sequences of their inserts suggest that bacteria present in the mine environment belong to: Flavobacteria, Sphingobacteriia, Bacteroides, Proteobacteria, Mollicutes and Firmicutes. The metagenomic approach allows to demonstrate a higher diversity of microbiota than classical microbiological studies of cultivable isolates.

Bacterial Communities from the Arsenic Mine in Złoty Stok, Sudety Mountains, Poland

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Investigations of bacterial communities and characterization of mineralogy of the environment in the Złoty Stok As-Au deposit were carried out. PXRD analysis...

Bacterial Communities from the Arsenic Mine in Złoty Stok, Sudety Mountains, Poland

Data publikacji: 27 wrz 2017

Zakres stron: 375 - 381

Otrzymano: 09 lut 2017

Przyjęty: 17 maj 2017

DOI: https://doi.org/10.5604/01.3001.0010.4875

Słowa kluczowe
arsenic mine ecosystem, metagenomic approach, microbial community, supergene minerals

© 2017 TOMASZ CŁAPA et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Bacterial Communities from the Arsenic Mine in Złoty Stok, Sudety Mountains, Poland

Data publikacji: 27 wrz 2017

Zakres stron: 375 - 381

Otrzymano: 09 lut 2017

Przyjęty: 17 maj 2017

DOI: https://doi.org/10.5604/01.3001.0010.4875

Słowa kluczowearsenic mine ecosystem, metagenomic approach, microbial community, supergene minerals

© 2017 TOMASZ CŁAPA et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Słowa kluczowe
arsenic mine ecosystem, metagenomic approach, microbial community, supergene minerals